蒙古冰草×航道冰草杂种F4代株系同工酶分析

doi:10.11733/j.issn.1007-0435.2006.01.004

›› 2006, Vol. 14 ›› Issue (1): 14-19.DOI: 10.11733/j.issn.1007-0435.2006.01.004

蒙古冰草×航道冰草杂种F₄代株系同工酶分析

权威, 于卓, 王月华, 马艳红, 李小雷

内蒙古农业大学农学院, 内蒙古, 呼和浩特, 010019

收稿日期:2005-04-10 修回日期:2005-07-11 出版日期:2006-02-15 发布日期:2006-02-15
通讯作者: 于卓,E-mail:yuzhuo58@sina.com
作者简介:权威(1980- ),女,蒙古族,内蒙古呼伦贝尔市莫旗人,硕士研究生,从事牧草饲用作物遗传育种研究,E-mail:quanwei360@sina.com;
基金资助:
教育部春晖计划资助项目(Z2004-1-15012);内蒙古高等学校科学研究重点领域资助项目(ZD01043)

Isozyme Analysis on Lines of F₄ Hybrid between Agropyron Mongolicum and A. Cristatum cv. Fairway

QUAN Wei, YU Zhuo, WANG Yue-hua, MA Yan-hong, LI Xiao-lei

College of Agronomy, Inner Mongolia Agriculture University, Huhhot, Inner Mongolia Autonomous Region 010019, China

Received:2005-04-10 Revised:2005-07-11 Online:2006-02-15 Published:2006-02-15

摘要/Abstract

摘要： 利用同工酶电泳技术研究蒙古冰草与航道冰草种间杂交F_{4代11个株系的遗传差异性。结果表明:在相同或不同生育阶段,供试材料的酯酶同工酶(EST)、过氧化物酶同工酶(POD)和超氧化物岐化酶(SOD)的位点、数目和强弱均存在差异,其表型差异是11个株系间及其与亲本间在蛋白质分子水平识别的重要遗传标记;选择抽穗期、分蘖期取样分析的同工酶酶谱特征,更能体现材料间酶谱表型的遗传差异性和提高鉴定结果的准确性;以遗传距离(GD)值0.40为基准,将13个材料聚类成6类:第1类株系6、7、9、8和10,第2类株系4和5,第3类株系1、3和2,第4类株系11,第5类父本航道冰草,第6类母本蒙古冰草;该研究结果对冰草杂交后代新品系的归类选育具有理论意义。}

关键词: 冰草, 杂种F_4, 株系, 同工酶酶谱, 遗传距离

Abstract: The genetic differences of 11 lines of F_{4 hybrid between Agropyron Mongolicum and A.cristatum were studied using isozyme electrophoresis technique.The results showed that there were certain differences in the number,locus and intensity of the esterase（EST）,proxidase（POD） and superoxide dismutase（SOD） isozyme bands among the tested materials at different developing stages.The differences of isozyme zymograms phenotypes were important genetic markers in protein level to the 11 lines of F₄ and parents;the isozyme zymograms characteristics of tested plants at earing and tillering stages in the summer and the autumn could accurately reflect their genetic differences and enhance the preciseness of identified results.According to the genetic distance standard of 0.40,the 13 tested plants were divided into 6 groups:lines 6,7,8,9 and 10 were in the first group,lines 4 and 5 in the second group,lines 1,3 and 2 in the third group,lines 11 was in the forth group,the male parent A.cristatum in the fifth group,and the female parent Agropyron mongolicum in the sixth group.The results had important theoryetic significance for clustering and breeding new wheatgrass cultivars.}

Key words: Wheatgrass, F_{4 hybrid,} Lines, Isozyme zymograms, Genetic distance

中图分类号:

S812
Q943

权威, 于卓, 王月华, 马艳红, 李小雷. 蒙古冰草×航道冰草杂种F₄代株系同工酶分析[J]. , 2006, 14(1): 14-19.

QUAN Wei, YU Zhuo, WANG Yue-hua, MA Yan-hong, LI Xiao-lei. Isozyme Analysis on Lines of F₄ Hybrid between Agropyron Mongolicum and A. Cristatum cv. Fairway[J]. , 2006, 14(1): 14-19.

参考文献

[1] Quiros C F.Starch gel electrophoresis technique used with alfalfa and other Medicago species[J].Can,Jour,Plant Sci.,1981,61:745-749
[2] Hazard L,Ghesouiere M.Evidence from the use of isozyme markers of competition in swards between short-leaved and longleaved perennial ryegrass[J].Grass and Forage Science,1995,50:241-248
[3] 杨静,石凤翎,张晓丽.缘毛雀麦各类型同工酶比较分析[J].中国草地,2002,24(5):45-47
[4] 李阳春,谢可军.早熟禾属10种植物酯酶同工酶分析[J].草地学报,2003,11(3):214-219
[5] 李昌林,陈默君,颜艳.二色胡枝子品种SODPOD同工酶的酶谱分析[J].草地学报,2003,11(2):210-213
[6] 马艳红,于卓,赵晓杰,等.加拿大披碱草-野大麦三倍体杂种加倍植株的同工酶分析[J].草地学报,2004,12(2):98-102
[7] 李造哲,马青枝,云锦凤,等.披碱草和野大麦及其杂种F1与BC1过氧化物同工酶分析[J].草业学报,2001,10(3):38-41
[8] 于卓,李造哲,云锦凤.几种小麦族禾草及其杂种后代农艺特性的研究[J].草业学报,2003,12(3):83-89
[9] 于卓,云锦凤.小麦族内几种远缘禾草及其杂种过氧化物酶同工酶分析[J].中国草地,1999,21(2):7-10
[10] 王月华,于卓,王四清,等.蒙古冰草×航道冰草杂种F3代的育性及染色体构型分析[J].中国草地,2003,25(2):1-7
[11] 陈玉林,卢得仁.同工酶及其在牧草研究中的应用[J].草业科学,1990,7(6):50-54
[12] 刘芳,孙根楼,颜济,等.普通小麦和华山新麦草及其属间杂种F1同工酶分析[J].作物学报,1992,18(3):169-174
[13] Quiros C F.Identification of alfalfa plants by enzyme ectrophoresis[J].Crop Sci.,1980,20:262-264
[14] Nei M.Mathemaucal model for studing geneon variation in terms of restriction emcomucleaseproc[M].Nati,Acad,Sci.,USA,1979.76:5269-5273
[15] 罗广华,王爱国.植物SOD的凝胶电泳及活性的显示[J].植物生理学通讯,1983,(6):44-45
[16] Yamashita M,Shimamoto A J.Variation and differentiation of allelic frequency of idozyme loci in perennial regrasss(Lolium perenne)[J].Journal of Japanese Society of Grassland Science,1993,39(4):459-468

蒙古冰草×航道冰草杂种F₄代株系同工酶分析

Isozyme Analysis on Lines of F₄ Hybrid between Agropyron Mongolicum and A. Cristatum cv. Fairway

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	范菠菠, 张学峰, 于卓, 赵彦, 马艳红. 与蒙古冰草抗旱相关的NAC转录因子生物信息学及其表达分析[J]. 草地学报, 2021, 29(6): 1183-1192.
[2]	王星, 宋珂辰, 许冬梅, 李永康, 撒春宁, 杨越. 荒漠草原人工柠条对冠下蒙古冰草种子萌发和幼苗生长的影响[J]. 草地学报, 2021, 29(11): 2470-2476.
[3]	袁继红, 凌巧, 李海燕, 杨允菲. 松嫩平原根茎冰草同生群无性系构件的生长可塑性及分配规律[J]. 草地学报, 2018, 26(4): 811-817.
[4]	见妮, 刘璐, 屈佳欣, 李凯, 王佺珍. 超声波对镉胁迫鸭茅和冰草种子萌发的影响[J]. 草地学报, 2018, 26(2): 427-434.
[5]	敖特根白音, 高立杰, 张树华, 云锦凤, 郎明林, 杨学举. 蒙古冰草肌动蛋白基因(MwACT2)克隆与表达分析[J]. 草地学报, 2016, 24(1): 129-136.
[6]	赵彦, 窦全文, 云锦凤, 王俊杰. 蒙古冰草Afa家族串联重复序列克隆及染色体定位[J]. 草地学报, 2015, 23(3): 448-452.
[7]	赵彦, 陈雪英, 石凤敏, 云锦凤, 王俊杰. 蒙古冰草MwDREB3基因的克隆及表达分析[J]. 草地学报, 2015, 23(2): 377-382.
[8]	于肖夏, 马艳红, 于卓, 李造哲. 高丹草染色体诱变株系的细胞学及SSR分析[J]. 草地学报, 2014, 22(5): 924-928.
[9]	张一弓, 贾纳提, 李学森, 张荟荟, 沙吾列, 马海燕, 唐森. 碱胁迫下塔乌库姆冰草的萌发及生理响应[J]. 草地学报, 2014, 22(4): 783-788.
[10]	罗丽朦, 王瑾, 王丽学, 秦立刚, 王堃. 扁穗冰草根鞘与其环境土壤理化性质和微生物数量的比较[J]. , 2013, 21(6): 1109-1112.
[11]	高悦, 朱永铸, 杨志民, 杜红梅. 干旱胁迫和复水对冰草相关抗性生理指标的影响[J]. , 2012, 20(2): 336-341.
[12]	张楠, 张林生, 邢媛, 刘兰, 李嘉宏. 扁穗冰草脱水素基因的克隆和表达特性分析[J]. , 2012, 20(1): 139-145.
[13]	金晓明, 敖永贵, 艾琳, 宋雪峰, 王瑞峰, 卢欣石. 呼伦贝尔沙地米氏冰草种群构件年龄结构动态[J]. 草地学报, 2011, 19(3): 367-371.
[14]	贺小霞, 刘一明, 王兆龙. 海滨雀稗栽培品种的形态特征与AFLP分子标记分析[J]. , 2011, 19(1): 164-170.
[15]	柴青, 钟芳, 李春杰, 南志标. 不同土壤湿度条件下微生物菌肥对3个绿化草种生长的影响[J]. , 2010, 18(6): 854-858.