蒙古冰草脱水素基因生物信息学识别及表达分析

doi:10.11733/j.issn.1007-0435.2024.03.004

草地学报 ›› 2024, Vol. 32 ›› Issue (3): 684-692.DOI: 10.11733/j.issn.1007-0435.2024.03.004

蒙古冰草脱水素基因生物信息学识别及表达分析

翟永青¹, 马艳红¹, 范菠菠¹, 房永雨², 张学峰¹, 于卓¹, 李坤¹, 吴婧¹, 闫秀秀¹, 赵彦³

1. 内蒙古农业大学农学院, 内蒙古呼和浩特 010019;
2. 内蒙古自治区农牧业科学院草原研究所, 内蒙古呼和浩特 010031;
3. 内蒙古农业大学草原与资源环境学院, 内蒙古呼和浩特 010018

收稿日期:2023-06-30 修回日期:2023-09-15 出版日期:2024-03-15 发布日期:2024-04-03
通讯作者: 马艳红,E-mail:mayanhong80@126.com
作者简介:翟永青(1999-)，女，汉族，山西大同人，硕士研究生，主要从事牧草遗传育种研究，E-mail：zhaiyongqing66@126.com
基金资助:
国家自然科学基金(31860670和32360338)资助

Bioinformatics Identification and Expression Analysis of Dehydratin Genes in Agropyron mongolicum

ZHAI Yong-qing¹, MA Yan-hong¹, FAN Bo-bo¹, FANG Yong-yu², ZHANG Xue-feng¹, YU Zhuo¹, LI Kun¹, WU Jing¹, YAN Xiu-xiu¹, ZHAO Yan³

1. College of Agriculture, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010019, China;
2. Institute of Grassland, Inner Mongolia Academy of Agriculture and Animal Husbandry Sciences, Hohhot, Inner Mongolia 010031, China;
3. College of Grassland andresources and Environment, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China

Received:2023-06-30 Revised:2023-09-15 Online:2024-03-15 Published:2024-04-03

摘要/Abstract

摘要： 为挖掘蒙古冰草(Agropyron mongolicum Keng)干旱胁迫响应的脱水素基因,本研究在转录组测序数据的基础上,利用生物信息学方法对蒙古冰草脱水素基因进行理化性质、亚细胞定位、保守基序、系统发育进化分析,对不同干旱处理的脱水素基因进行差异表达分析。结果表明:共筛选到6个具有脱水素蛋白保守结构域的蒙古冰草脱水素基因,蛋白大小在 81~275个氨基酸残基之间;分子量在 0.83~2.75 kD之间;理论等电点在5.14~10.00 之间;6个脱水素基因预测均位于细胞核;AmDHNs基因编码的蛋白均包括motif1和motif2;系统进化树发现DN14936_c0_g6,DN18948_c1_g4与已知具有抗旱功能的基因具有较高同源性。qRT-PCR分析与对照(CK)相比5个基因在干旱处理下显著差异表达,1个基因的表达量差异不显著,综合转录组测序数据和qRT-PCR分析表明6个基因的表达量随干旱处理时间延长总体呈上升趋势。本研究可为蒙古冰草抗旱相关脱水素基因的生物学功能验证提供理论基础。

关键词: 蒙古冰草, 脱水素基因, 生物信息学分析, 干旱胁迫

Abstract: In order to excavate the dehydrin genes in response to drought stress in Agropyron mongolicum Keng (Agropyron mongolicum Keng),this study used bioinformatic methods to analyze the physicochemical properties,subcellular localization,conserved motifs,and phylogenetic evolution of the dehydrin genes of Agropyron mongolicum Keng. And the study analyzed the differential expression of the dehydrin genes in response to different drought treatments,on the basis of the transcriptome sequencing data. The results showed that:six Agropyron mongolicum Keng dehydrin genes with conserved structural domains of dehydrin proteins were screened,and the protein sizes ranged from 81 to 275 amino acid residues;the molecular weights ranged from 0.83 to 2.75 kD;the theoretical isoelectric points ranged from 5.14 to 10.00;the six dehydrin genes were predicted to be located in the nucleus;the proteins encoded by AmDHNs genes were all included motif1 and motif2;the phylogenetic tree revealed that DN14936_c0_g6 and DN18948_c1_g4 had high homology with genes known to have drought tolerance functions. qRT-PCR analysis showed that five genes were significantly differentially expressed under drought treatment compared with the control (CK),and one gene was not significantly expressed. The combined transcriptome sequencing data and qRT-PCR analysis indicated that the expression of the six genes varied with drought treatment. The present study can provide a theoretical basis for the validation of the biological functions of drought-related dehydrin genes in Agropyron mongolicum Keng.

Key words: Agropyron mongolicum Keng, Dehydrin, Bioinformatics analysis, Drought stress

中图分类号:

S543+.9

翟永青, 马艳红, 范菠菠, 房永雨, 张学峰, 于卓, 李坤, 吴婧, 闫秀秀, 赵彦. 蒙古冰草脱水素基因生物信息学识别及表达分析[J]. 草地学报, 2024, 32(3): 684-692.

ZHAI Yong-qing, MA Yan-hong, FAN Bo-bo, FANG Yong-yu, ZHANG Xue-feng, YU Zhuo, LI Kun, WU Jing, YAN Xiu-xiu, ZHAO Yan. Bioinformatics Identification and Expression Analysis of Dehydratin Genes in Agropyron mongolicum[J]. Acta Agrestia Sinica, 2024, 32(3): 684-692.

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蒙古冰草脱水素基因生物信息学识别及表达分析

Bioinformatics Identification and Expression Analysis of Dehydratin Genes in Agropyron mongolicum

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