草地学报

›› 2010, Vol. 18 ›› Issue (5): 731-734.DOI: 10.11733/j.issn.1007-0435.2010.05.022

• 研究论文 • 上一篇    下一篇

瑞香狼毒根对长爪沙鼠的急性毒力测试

史琨1, 周大森1, 郭永旺2, 施大钊1, 王登1   

  1. 1. 中国农业大学农学与生物技术学院, 北京, 100193;
    2. 全国农业技术推广中心, 北京, 100026
  • 收稿日期:2010-06-03 修回日期:2010-09-20 出版日期:2010-10-15 发布日期:2010-10-15
  • 通讯作者: 王登,E-mail:wangdeng@cau.edu.cn
  • 作者简介:史琨(1989- ),女,山西平遥人,学士,E-mail:kellyfour@yeah.net
  • 基金资助:
    国家自然科学基金项目(30800727);全国农区统一灭鼠项目资助

Acute Toxicity Analyses of Stellera chamaejasme Root in Mongolian Gerbils(Meriones unguiculatus)

SHI Kun1, ZHOU Da-sen1, GUO Yong-wang2, SHI Da-zhao1, WANG Deng1   

  1. 1. College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China;
    2. National Agro-tech Extensions and Service Center, Beijing 100026, China
  • Received:2010-06-03 Revised:2010-09-20 Online:2010-10-15 Published:2010-10-15

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摘要： 本文采用含17%瑞香狼毒(Stellera chamaejasme)根粉末的毒饵,依据孙氏改良寇氏综合计算法,实验室内测定了其对长爪沙鼠(Meriones unguiculatus)的急性毒力。根据预试验结果,采用最低剂量为7.0 g·kg-1,最适剂量梯度比值K=0.65的5个剂量梯度的瑞香狼毒根毒饵饲喂长爪沙鼠。结果显示:试验组试鼠体重明显下降(P=0.029<0.05),完全致死组肝脏重/体重比对照组明显减小(P=0.014<0.05),但肾脏略增大(P=0.102>0.05)。其对长爪沙鼠的LD50为(20.37±0.086)g·kg-1,95%平均可信限为(20.37±7.84)g·kg-1,属弱毒水平。

关键词: 瑞香狼毒, 长爪沙鼠, 生物农药, 致死中量

Abstract: Both whole Stellera chamaejasme and its extract are demonstrated to have powerful toxicity to some pest insects.The poison bait containing 17% roots of Stellera chamaejasme was used to test the acute toxicity of Stellera chamaejasme in Mongolian gerbils according to Sun′s improved Kou′s Integrated Way.Mongolian gerbils were treated with 5 treatments of toxicant bait according to pretested results: the lowest dose was 7.0 g/kg and the optimal gradient dose radio was 0.65.Results indicate that the body weight of treatment group was significantly decreased as compared to control(P=0.029<0.05).The rate of liver to body weight was significantly decreased in groupⅤ(P=0.014<0.05,whereas,the percent of kidney to body weight was slightly increased in groupⅤ(P=0.102>0.05) compared with controls.The LD50 of Stellera chamaejasme in Mongolian gerbils is 20.37±0.086 g/kg.The confidence limit on 95% is(20.37±7.84) g/kg.The acute virulence is slight as relates to the graded standard of toxicant against mice.

Key words: Stellera chamaejasme, Mongolian gerbils, Botanical pesticide, Median lethal dose(LD50)

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