草地学报

›› 2011, Vol. 19 ›› Issue (5): 776-780.DOI: 10.11733/j.issn.1007-0435.2011.05.011

• 研究论文 • 上一篇    下一篇

不同光强对4种草坪草类胡萝卜素的影响

杨芳1, 孙小玲2, 周禾1   

  1. 1. 中国农业大学草地研究所, 北京100193;
    2. 天津农学院园艺系, 天津712100
  • 收稿日期:2011-04-12 修回日期:2011-05-25 出版日期:2011-10-15 发布日期:2012-07-12
  • 通讯作者: 周禾,E-mail:zhouhe@cau.edu.cn
  • 作者简介:杨芳(1986- ),女,湖南娄底人,硕士研究生,研究方向为草坪植物逆境生理生态学,E-mail:yf5185@126.com

Influences of Wavelength and Intensity on Carotenoid of Four Lawn Grass

YANG Fang1, SUN Xiao-ling2, ZHOU He1   

  1. 1. Department of Grassland Science, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;
    2. Department of Horticulture, Tianjin Agriculture College, Tianjin 300384, China
  • Received:2011-04-12 Revised:2011-05-25 Online:2011-10-15 Published:2012-07-12

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摘要： 为明确不同光照强度对不同遗传背景的草种体内的色素比值的影响,设33%,66%,100%正常光强3个光强梯度处理沿阶草(Othiopogon japanicus)、高羊茅(Festuca arundinacea Shreb.cv.ruby)、崂峪苔草(Carex giraldiana)和紫羊茅(Festuca vubra L.cv.boreal)草坪草,采用高效液相色谱仪和Waters Spherisorb ODS-2 columns封口色谱柱,测定各草种色素的相对含量,并比较叶绿素a/b(Chla/Chlb)、黄体素/叶黄素库(L/V)、α-胡萝卜素及其衍生物/β-胡萝卜素及其衍生物(αC/βC)和类胡萝卜素/叶绿素(Car/Chl)。结果表明:随着光照强度的减弱,4种植物体内的Chla/Chlb比值在逐渐减少,αC/βC比值反而增加,Chla/Chlb,L/V,Car/Chl在4草种间差异不显著,αC/βC比值在不同遮荫强度下各草种间差异显著(P<0.05),沿阶草的比值最低,紫羊茅的最高,因此可将α-胡萝卜素及其衍生物/β-胡萝卜素及其衍生物的比值作为植物耐荫性的指标。

关键词: 草坪草, 耐荫, 类胡萝卜素, αC/βC

Abstract: Light intensities of 33,66 and 0 percentages are used to explore the importance of pigment ratio in grass seed under different light conditions and genetic backgrounds of four types of different lawn grass: Othiopogon japanicus;Festuca Arundinacea Shreb.cv.ruby;Carex giraldiana and Festuca vubra L.cv.boreal.The relative contents of different grass pigments,Chla/Chlb,L/V,αC/βC and Car/Chl were measured and compared by HPLC and Waters Spherisorb ODS-2 columns.Result demonstrated that the ratio of Chla/Chlb in each grass species decreased as light intensity decreased,while the ratio of αC/βC in each grass species increased.The ratios of Chla/Chlb,L/V and Car/Chl in 4 grass species were not significantly different.The ratio of αC/βC is significantly different among grass species under shadow intensity of light(P<0.05).The αC/βC ratio of Othiopogon japanicus was the lowest,while Festuca vubra L.cv.boreal had the highest.Therefore,the ratio of α-carotene and its derivatives/β-carotene and its derivatives can be used as a shade-tolerance indicator of plant.

Key words: Lawn grass, Shade-tolerance, Carotene, αC/βC

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