8个小麦条锈菌生理小种产孢相关基因PsCon1克隆及表达分析

doi:10.11733/j.issn.1007-0435.2013.02.027

›› 2013, Vol. 21 ›› Issue (2): 388-393.DOI: 10.11733/j.issn.1007-0435.2013.02.027

8个小麦条锈菌生理小种产孢相关基因PsCon1克隆及表达分析

司二静^1,2, 赖勇^1,2, 孟亚雄^1,2, 李葆春^1,2, 马小乐^1,2, 尚勋武², 王化俊^1,2

1. 甘肃省作物遗传改良与创新重点实验室甘肃省干旱生境作物学重点实验室, 甘肃兰州 730070;
2. 甘肃农业大学农学院生命科学技术学院, 甘肃兰州 730070

收稿日期:2012-09-21 修回日期:2012-12-21 出版日期:2013-04-15 发布日期:2013-04-23
通讯作者: 王化俊,whuajun@yahoo.com
作者简介:司二静(1984-),男,山西晋城人,博士研究生,研究方向为麦类分子抗性遗传与育种,E-mail:sierjing@163.com
基金资助:
国家自然基金(30771331);甘肃省农业生物技术研究与应用开发项目(GNSW200701);甘肃省干旱生境作物学重点实验室开放基金(GSCS-2010)资助

Cloning and Expression Analysis Conidiation-Related Gene Pscon1 from Eight Physiological Races of Puccinia striiformis f.sp.tritici

SI Er-jing^1,2, LAI Yong^1,2, MENG Ya-xiong^1,2, LI Bao-chun^1,2, MA Xiao-le^1,2, SHANG Xun-wu², WANG Hua-jun^1,2

1. Gansu Key Lab.of Crop Important & Germplasm Enhancement/Gansu Provincial Key Laboratory of Aridl and Crop Science, Lanzhou, Gansu Province 730070, China;
2. College of Agronomy/College of Life Science and Technology, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China

Received:2012-09-21 Revised:2012-12-21 Online:2013-04-15 Published:2013-04-23

摘要/Abstract

摘要： 为研究基因Pscon1在不同小种中核苷酸序列及相对表达量差异,以小麦条锈菌(Puccinia striiformis f. sp. tritici)基因组DNA为模板,在小麦条锈菌8个生理小种中分别克隆产孢相关基因Pscon1,并对其核苷酸和氨基酸序列进行比对及相对表达量分析。结果表明:在8个小种中分别克隆到条锈菌产孢相关基因Pscon1,基因大小为531 bp,由3个外显子和2个内含子构成,开放阅读框长252 bp,不同小种基因Pscon1核苷酸序列只在44,65,331,465,495和510 bp位点处发生突变,均未引起氨基酸序列变化,且不同生理小种Pscon1在接种'铭贤169' 12 h后相对表达量有差异,水源11-3最高,最低的是Hybrid46-8。推测基因Pscon1在小麦条锈菌不同生理小种产孢过程中相对表达量有差异,这将为进一步研究产孢量差异因素提供参考。

关键词: 小麦条锈菌, 生理小种, 产孢相关基因, 实时定量PCR

Abstract: In order to research the difference of nucleotide and relative expression level between different races, Puccinia striiformis f. sp. tritici was used as the DNA source. Conidiation-related gene Pscon1 was cloned and isolated from eight epidemic physiological races, and nucleotide and amino acid level and relative expression levels were analyzed. Results indicated that a 531 bp DNA sequence of PsConl was cloned and comprised three extrons and two introns,including a complete 252 bp open reading frame. Sequence analysis indicated that the PsConl sequences of tested physiological races had a few mutations in the site of 44 bp, 65 bp, 331 bp, 465 bp, 495 bp and 510 bp, but no amino acid sequences were changed. The relative expression levels of tested physiological races were different after 12 h inoculation. Accession Su11-3 had the greatest expression while Hybrid46-8 had the least among tested physiological races. It was concluded that the effect of conidiation-related gene Pscon1 on the spore production of different physiological races was different.

Key words: Wheat stripe rust (Puccinia striiformis f. sp. tritici), Physiological race, Conidiation-related gene, Real time PCR

中图分类号:

司二静, 赖勇, 孟亚雄, 李葆春, 马小乐, 尚勋武, 王化俊. 8个小麦条锈菌生理小种产孢相关基因PsCon1克隆及表达分析[J]. , 2013, 21(2): 388-393.

SI Er-jing, LAI Yong, MENG Ya-xiong, LI Bao-chun, MA Xiao-le, SHANG Xun-wu, WANG Hua-jun. Cloning and Expression Analysis Conidiation-Related Gene Pscon1 from Eight Physiological Races of Puccinia striiformis f.sp.tritici[J]. , 2013, 21(2): 388-393.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://manu40.magtech.com.cn/Jweb_cdxb/CN/10.11733/j.issn.1007-0435.2013.02.027

https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2013/V21/I2/388

参考文献

[1] 李振歧,商鸿生.小麦锈病及其防治[M].上海:上海科学技术出版社,1989:2-3
[2] 李振岐,曾士迈.中国小麦锈病[M].北京:中国农业出版社,2002:270-271
[3] 李振歧.我国小麦品种抗条锈性丧失原因及其解决途径[J].中国农业科学,1980,13(3):72- 76
[4] 曾士迈.植物病原菌寄生适合度测定方法的研究:以小麦条锈菌为例[J].植物病理学报,1996,26(2):97-104
[5] 王保通,李高宝,李强,等.中国小麦条锈菌主要流行菌系的寄生适合度研究[J].植物病理学报,2009,39(1):82-87
[6] 廖奇亨,贺春萍,林春花,等.稻瘟病菌产孢相关基因研究进展[J].热带农业科学,2005,25(3):57-63
[7] White B T, Yanofsky C. Structural characterization and expression analysis of the Neurospora conidiation gene con-6. Developmental Biology,1993,160(1):254-264
[8] 陈玥颖,郭军,代西维,等.小麦条锈菌一个产孢相关基因Pscon1的克隆及表达特征分析[J].中国农业科学,2010,43(6):1156-1163
[9] 刘静,李中元,王军娥,等.丝状真菌产孢机制及其相关基因研究进展[J].贵州农业科学,2009,37(4):81-83
[10] Roberts A N, Yanofsky C. Genes expressed during conidiation in Neurospora crassa: Characterization of con-8[J]. Nucleic Acids Research,1989,17(1):197-214
[11] Hager K M, Yanofsky C. Genes expressed during conidiation in Neurospora crassa: Molecular characterization of con-13[J]. Gene,1990,96(2):153-159
[12] Madi L, Ebbole D J, White B T. Mutants of Neurospora crassa that alter gene expression and conidia development[J]. Proceedings of the National Academy of Sciences of the United States of America,1994,91(13):6226-6230
[13] Madi L, McBride S A, Bailey L A. rco-3, a gene involved in glucose transport and conidiation in Neurospora crassa[J]. Genetics,1997,146(2):499-508
[14] Bailey-Shrode L, Ebbole D J. The fluffy gene of Neurospora crassa is necessary and sufficient to induce conidiophores development[J]. Genetics,2004,166(4):1741-1749
[15] Bieszke J A, Li L, Borkovich K A. The fungal opsin gene nop-1 is negatively-regulated by a component of the blue light sensing pathway and influences conidiation-specific gene expression in Neurospora crassa[J]. Current Genetics,2007,52(3/4):149-157
[16] Adams T H, Wieser J K, Yu J H. Asexual sporulation in Aspergillus idulans[J]. Microbiology Molecular Biology Reviews,1998,62(1):35-54
[17] Ruiz-Roldan M C, Maier F J, Schafer W. PTK1, a mitogen-activated-protein kinase gene, is required for conidiation,appressorium formation, and pathogenicity of Pyrenophora teres on barley [J]. Molelular Plant-Microbe Interactions,2001,4(2):16-125
[18] 吴素真.盾壳霉产袍相关基因的克隆及液体产袍条件的优化[D].武汉:华中农业大学,2006:26-30
[19] 王萍萍.产孢相关基因的克隆及腺苷脱氨酶(CMADA)基因的初步研究[D].武汉:华中农业大学,2010:42-45
[20] 康振生,李振岐.洛夫林10常温致病菌系的发现[J].西北农学院学报,1984,12(4):18-28
[21] 韩冰,蔺瑞明,曹远银,等.小麦条锈菌提取方法的比较[J].中国农学通报,2005,22(4):81-83
[22] 李文龙,郭述茂,王晶,等. 基于集合种群模型的小麦和苜蓿锈病发生动态模拟研究[J].草业学报,2009,18(2):46-51
[23] 孟亚雄,仲军,马小乐,等.甘肃省小麦条锈病主要流行小种的RAPD分析及Sull-4小种SCAR标记建立[J].草业学报, 2010,19(6):180-186
[24] Nelson R R. The evolution of parasite fitness [M]//Horsfall J G, Cowling E B, eds. Plant disease-A advanced treatise, Vol Ⅳ. New York: Academic Press,1979:23-26
[25] Berlin V, Yanofsky C. Isolation and characterization of genes differentially expressed during conidiation of Neurospora crassa [J]. Molecular Cell Biology,1985,5(4):849-855

8个小麦条锈菌生理小种产孢相关基因PsCon1克隆及表达分析

Cloning and Expression Analysis Conidiation-Related Gene Pscon1 from Eight Physiological Races of Puccinia striiformis f.sp.tritici

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 1

编辑推荐

Metrics

本文评价