对啶酰菌胺敏感性不同的币斑病菌致病性分析

doi:10.11733/j.issn.1007-0435.2021.06.008

草地学报 ›› 2021, Vol. 29 ›› Issue (6): 1193-1199.DOI: 10.11733/j.issn.1007-0435.2021.06.008

对啶酰菌胺敏感性不同的币斑病菌致病性分析

赵正阳¹, 索欣¹, 陈玲¹, 刘雄洲¹, 胡健², 尹淑霞¹

1. 北京林业大学草业与草原学院, 北京 100083;
2. 南京农业大学草业学院, 江苏南京 210095

收稿日期:2021-01-14 修回日期:2021-03-09 出版日期:2021-06-15 发布日期:2021-07-05
通讯作者: 尹淑霞,E-mail:yinsx369@bjfu.edu.cn
作者简介:赵正阳(1996-)，男，汉族，河南新乡人，硕士研究生，主要从事草坪病害综合防治研究，E-mail：1137245624@qq.com
基金资助:
国家自然科学基金（31701810）资助

Pathogenicity Analysis of Clarireedia spp. with Different Sensitivities to Boscalid

ZHAO Zheng-yang¹, SUO Xin¹, CHEN Ling¹, LIU Xiong-zhou¹, HU Jian², YIN Shu-xia¹

1. School of Grassland Science, Beijing Forestry University, Beijing 100083, China;
2. College of Agro-grassland Science, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China

Received:2021-01-14 Revised:2021-03-09 Online:2021-06-15 Published:2021-07-05

摘要/Abstract

摘要： 为探究对啶酰菌胺敏感性不同的草坪币斑病菌致病性，本研究根据病原菌对啶酰菌胺的敏感性测定结果，将从天津的高尔夫球场匍匐翦股颖（Agrostis stolonifera）草坪上分离纯化的106株币斑病菌分为高抗（HR）、低抗（LR）、敏感（SS）类型，并在每种类型各挑选2株菌测定菌丝生长速率、生物量、草酸产量，以及接种匍匐翦股颖后的发病率、病情指数，叶片丙二醛（malondialdehyde，MDA）含量。结果表明，对啶酰菌胺敏感性不同的菌株，其致病性存在显著差异。与低抗菌株和敏感菌株相比，高抗菌株生长速率低25.2%左右，生物量少32.4%以上，草酸产量降低27.3%以上，接种匍匐翦股颖后发病率降幅为37.5%以上，病情指数减小49.5%~84.1%，叶片MDA含量降低38.4%以上。总体上看，高抗菌株的致病性最弱，敏感菌株的致病性最强，低抗菌株的致病性介于二者之间。研究结果为草坪币斑病菌对啶酰菌胺的抗性风险评价提供了参考。

关键词: 币斑病菌, 匍匐翦股颖, 啶酰菌胺敏感性, 致病力

Abstract: In order to explore the pathogenicity of Clarireedia with different sensitivities to boscalid,the 106 strains of dollar spot pathogens collected from creeping bentgrass(Agrostis stolonifera) in golf courses of Tianjin were classified into three resistance phenotypes:high resistance (HR)，low resistance (LR) and sensitive (SS) strains based on their resistance to boscalid in this study,two strains from each of the above resistance phenotypes,were selected, and the hyphae growth rate,biomass,oxalic acid production of these pathogens were measured;the disease incidence,disease index and malondialdehyde (MDA) content of creeping bentgrass leaves were determined after creeping bentgrass were inoculated by the 6 strains, to evaluate the pathogenicity of pathogens with different sensitivities to boscalid. The results showed that the pathogenicity of dollar spot pathogen strains with different sensitivity to boscalid was significantly different. Compared to the LR and SS strains,the HR strains performed lower hyphae growth rate(decreased by 25.2%),lower biomass(decreased by more than 32.4%) and lower oxalic acid production (decreased by more than 27.3%). Lower diseases incidence(decreased by more than 37.5%), lower diseases index (decreased by 49.5%~84.1%) and lower MDA content in leaves (decreased by more than 38.4%) were found in HR in relative to LR and SS,after inoculating them into creeping bentagrass. In conclusion,the pathogenicity of strains with high resistance to boscalid was the lowest,the pathogenicity of strains that sensitive to boscalid was the highest,and that of LRs was between the two. The results provide useful information for risk assessment of Clarireedia resistance to boscalid.

Key words: Clarireedia, Creeping bentgrass, Sensitivity to boscalid, Pathogenicity

中图分类号:

S153

赵正阳, 索欣, 陈玲, 刘雄洲, 胡健, 尹淑霞. 对啶酰菌胺敏感性不同的币斑病菌致病性分析[J]. 草地学报, 2021, 29(6): 1193-1199.

ZHAO Zheng-yang, SUO Xin, CHEN Ling, LIU Xiong-zhou, HU Jian, YIN Shu-xia. Pathogenicity Analysis of Clarireedia spp. with Different Sensitivities to Boscalid[J]. Acta Agrestia Sinica, 2021, 29(6): 1193-1199.

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参考文献

[1] 李永. 浦绿和卡丽芭对匍匐翦股颖草坪的节水效果研究[J]. 安徽农业科学,2019,47(13):108-118
[2] 王慧慧,刘骐华,王婧,等. 4个匍匐翦股颖品种种子萌发期耐盐性评价[J]. 草原与草坪,2019(5):31-36
[3] 姬承东,张德罡,朱钧,等. 高温对匍匐翦股颖果岭草坪草生理特性及再生性的影响[J]. 中国农学通报,2007,23(1):221-224
[4] Rioux Renée A,Jeanette S,Michelle G. Sclerotinia homoeocarpa overwinters in turfgrass and is present in commercial seed[J]. PLoS ONE,2014,9(10):e110897
[5] 章武,刘国道,南志标. 4种暖季型草坪草币斑病病原菌鉴定及其生物学特性[J]. 草业学报,2015,24(1):124-131
[6] Salgado-Salazar C,Beirn L A,Ismaiel A. Clarireedia:A new fungal genus comprising four pathogenic species responsible for dollar spot disease of turfgrass[J]. Fungal Biology,2018(122):761-773
[7] Hu J,Zhou Y,Geng J. A new dollar spot disease of turfgrass caused by Clarireedia paspali[J]. Mycological Progress,2019,18(12):1423-1435
[8] 陈立坤,李学政,邓红华,等. 匍匐翦股颖币斑病的发生及综合防治[J]. 现代农业科技,2018(24):126-127
[9] Detweiler A R. Resistance of Sclerotinia homoeocarpa to iprodione and benomyl[J]. Plant Disease,1983,67(6):627-630
[10] Ostrander J C,Todd R B,Kennelly M M. Resistance of Kansas Sclerotinia homoeocarpa strains to thiophanate-methyl and determination of associated β-tubulin mutation[J]. Plant Health Progress,2014,15(2):23-27
[11] Hu J,Deng S,Gao T,et al. Thiophanate-methyl resistance in Sclerotinia homoeocarpa from golf courses in China[J]. Pesticide Biochemistry and Physiology,2018(152):84-89
[12] 王文桥,马志强,张小风,等. 植物病原菌对杀菌剂抗性风险评估[J]. 农药学学报,2001,3(1):6-11
[13] 潘以楼,朱桂梅,郭建,等. 油菜菌核病菌对啶酰菌胺的敏感性及对不同杀菌剂敏感性的相关分析[J]. 西南农业学报,2012,25(2):507-512
[14] Stephens C M,Kaminski J E. In Vitro fungicide-insensitive profiles of Sclerotinia homoeocarpa populations from Pennsylvania and the surrounding region[J]. Plant Disease,2018,103(2):214-222
[15] James T Popko Jr,Hyunkyu Sang,Jaemin Lee,et al. Resistance of Sclerotinia homoeocarpa field isolates to succinate dehydrogenase inhibitor fungicides[J]. Plant Disease,2018,102(12):2625-2631
[16] 聂秀美,赵桂琴,孙浩洋,等. 天祝县燕麦叶斑病的发生情况及其病原鉴定[J]. 草地学报,2019,27(5):1384-1391
[17] 胡健,杨静雅,李捷,等. 草坪草币斑病菌对甲基硫菌灵、异菌脲和丙环唑的敏感性[J]. 农药学学报,2017,19(6):694-700
[18] Ok C H,Popko J T,Campbell-Nelson K. In vitro assessment of Sclerotinia homoeocarpa resistance to fungicides and plant growth regulators[J]. Plant Disease,2011,95(1):51-56
[19] 刘欣,吴雁,成玮,等. 上海地区草莓灰霉病菌对啶酰菌胺的敏感性检测及抗性机制分析[J]. 农药学学报,2018,20(4):452-458
[20] 张丽燕.单一田块核盘菌多样性分析及携带真毒的菌株的遗传特性研究[D]. 武汉:华中农业大学,2010:22-24
[21] 梁宏杰. Strobilurins类杀菌剂吡唑醚菌酯对核盘菌生物活性的研究[D]. 武汉:华中农业大学,2015:33-34
[22] 蔡文涌,王肖肖,方香玲. 不同尖孢镰刀菌菌株对紫花苜蓿幼苗生长的影响[J]. 草地学报,2020,28(5):1233-1239
[23] 赵英杰,王东,赵鑫. 弱毒黄萎病菌的筛选及诱导向日葵交互保护作用的条件优化[J]. 中国生物防治学报,2019(1):112-119
[24] 王军,赵桂琴,柴继宽,等. BYDV侵染对燕麦抗氧化防御酶,苯丙氨酸解氨酶及多酚类氧化酶的影响[J]. 草地学报,2020,28(1):56-63
[25] 邓小军,李桂芳,魏颖,等. 油菜菌核病菌致病性分化及弱毒现象[J]. 湖南农业大学学报(自然科学版),2016,42(3):317-321
[26] 羊国根,程家森. 核盘菌致病机理研究进展[J]. 生物技术通报,2018,34(4):9-15
[27] Fagundes-Nacarath I R F,Debona D,Rodrigues F A. Oxalic acid-mediated biochemical and physiological changes in the common bean-Sclerotinia sclerotiorum interaction[J]. Plant Physiology Biochemistry,2018(129):109-121
[28] 黄娟,李国庆. 两株毒力不同的核盘菌产草酸、果胶酶的比较[J]. 氨基酸和生物资源,2008,30(2):5-8
[29] 许大凤,高智谋,张茜茹,等. 安徽省油菜菌核病菌致病力分化研究[J]. 安徽农业大学学报,2014,41(5):762-762
[30] 李琨. 稻白叶枯病菌弱毒菌株对水稻品种的抗病诱导效果及生化机制的研究[D]. 武汉:华中农业大学,2007:28-29
[31] 刘召阳,王帅,高宇琪,等. 2株不同地理来源的苹果树腐烂病菌对甾醇生物合成抑制剂类杀菌剂的交互抗药性及生物适合度分析[J]. 西北林学院学报,2020,35(2):119-124
[32] 代玉立,甘林,阮宏椿,等. 福建省丙环唑不同敏感性玉米小斑病菌的遗传多样性和致病性[J]. 植物病理学报,2019,49(1):64-74
[33] 聂峰杰,黄丽丽,康振生. 核盘菌弱毒现象及其生防潜能的研究进展[J]. 中国油料作物学报,2013,35(3):334-340

对啶酰菌胺敏感性不同的币斑病菌致病性分析

Pathogenicity Analysis of Clarireedia spp. with Different Sensitivities to Boscalid

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