ALA对高温胁迫下苜蓿属3个品种叶片生理的影响

doi:10.11733/j.issn.1007-0435.2022.05.019

草地学报 ›› 2022, Vol. 30 ›› Issue (5): 1178-1184.DOI: 10.11733/j.issn.1007-0435.2022.05.019

ALA对高温胁迫下苜蓿属3个品种叶片生理的影响

张鹤, 敖平星, 赵雁

云南农业大学园林园艺学院, 云南昆明 650201

收稿日期:2021-10-11 修回日期:2021-12-22 出版日期:2022-05-15 发布日期:2022-06-06
通讯作者: 赵雁,E-mail:zhaoyan@ynau.edu.cn
作者简介:张鹤(1996-),女,汉族,四川眉山人,硕士研究生,主要从事园林植物资源利用与创新研究,E-mail:zhanghe7282@163.com
基金资助:
国家自然科学基金项目(31660683)资助

Effect of 5-aminolevulinic Acid on Leaf Physiology of Three Cultivars of Medicago under High Temperature Stress

ZHANG He, AO Ping-xing, ZHAO Yan

College of Horticulture and Landscape of Yunnan Agricultural University, Kunming, Yunnan Province 650201, China

Received:2021-10-11 Revised:2021-12-22 Online:2022-05-15 Published:2022-06-06

摘要/Abstract

摘要： 为探究5-氨基乙酰丙酸(5-aminolevulinic acid，ALA)对苜蓿属(Medicago)植物耐热性的影响，以苜蓿属3个品种盆栽苗为材料、以常温无ALA (CK)和高温胁迫无ALA (Heat)为对照，研究不同浓度ALA (5，10，15，20，25 mg·L^-1)预处理对高温胁迫后叶片生理的影响。结果表明，42℃高温胁迫后，3个品种叶片的相对电导率和MDA含量显著升高(P<0.05)，其中WL525HQ、德钦苜蓿和楚雄南苜蓿相对电导率较CK分别升高205.844%，206.589%，226.741%，MDA含量则升高112.649%，110.140%，124.664%，且叶片严重失绿。但喷施不同浓度ALA后可有效减少MDA含量和相对电导率(P<0.05)，缓解叶片失绿，同时提高叶绿素a、b和总叶绿素含量(P<0.05)，并且叶片中SOD、POD和CAT活性较高(P<0.05)。通过双因素方差分析表明，在影响苜蓿各耐热性指标的因素中，处理和品种虽有显著影响，但其作用大小为处理>品种。以8个生理指标进行隶属函数分析表明，20 mg·L^-1ALA预处理对3个品种缓解高温胁迫的效果最好。

关键词: 5-氨基乙酰丙酸, 苜蓿属植物, 耐热性, 叶片生理

Abstract: To explore the effect of 5-aminolevulinic acid (ALA) on heat tolerance of Medicago plants. Three cultivars of potted seedlings of Medicago were used as materials to study the effects of different concentrations of ALA (5,10,15,20 and 25 mg·L^-1) pretreatment on the leaf physiology of Medicago plants after high temperature stress,with no ALA at room temperature (CK) and no ALA at high temperature stress (Heat) as controls. The results showed that the relative conductivity and MDA content of the leaves of the three varieties increased significantly (P<0.05) after high temperature stress at 42℃. Compared with CK,the relative conductivity of M. sativa 'WL525HQ',M. sativa'Deqin',M. hispida 'Chuxiong' increased by 205.844%,206.589% and 226.741%,respectively;and the MDA content increased by 112.649%,110.140% and 124.664%,respectively,and the leaves were severely chlorotic. However,spraying different concentrations of ALA effectively reduced MDA content and relative conductivity (P<0.05),reduced leaf greening while increasing chlorophyll a,b and total chlorophyll content (P<0.05),and higher SOD,POD and CAT antioxidant enzyme activities in the leaves (P<0.05).A two-way ANOVA showed that among the factors affecting each heat tolerance index in Medicago plant,treatment and variety had a significant effect,although the magnitude of the effect was:treatment > variety.Membership function analysis with 8 physiological indicators showed that 20 mg·L^-1 ALA pretreatment was most effective in alleviating high temperature stress in three cultivars.

Key words: ALA, Medicagoplant, Heat tolerance, Leaf physiology

中图分类号:

S541

张鹤, 敖平星, 赵雁. ALA对高温胁迫下苜蓿属3个品种叶片生理的影响[J]. 草地学报, 2022, 30(5): 1178-1184.

ZHANG He, AO Ping-xing, ZHAO Yan. Effect of 5-aminolevulinic Acid on Leaf Physiology of Three Cultivars of Medicago under High Temperature Stress[J]. Acta Agrestia Sinica, 2022, 30(5): 1178-1184.

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ALA对高温胁迫下苜蓿属3个品种叶片生理的影响

Effect of 5-aminolevulinic Acid on Leaf Physiology of Three Cultivars of Medicago under High Temperature Stress

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