草地学报

›› 2012, Vol. 20 ›› Issue (6): 1169-1174.DOI: 10.11733/j.issn.1007-0435.2012.06.030

• 研究论文 • 上一篇    

人工饲料对草地螟消化酶活性及羧酸酯酶mRNA表达量的影响

吴晋华1,2, 刘爱萍1, 徐林波1, 高书晶1, 韩冰2, 康爱国3, 张玉慧3   

  1. 1. 中国农业科学院草原研究所, 内蒙古 呼和浩特 010010;
    2. 内蒙占农业大学生命科学院, 内蒙古 呼和浩特 010018;
    3. 河北省康保县植保站, 河北 康保 076650
  • 收稿日期:2012-05-16 修回日期:2012-09-25 出版日期:2012-12-15 发布日期:2012-12-28
  • 通讯作者: 刘爱萍
  • 作者简介:吴晋华(1986-),女,山西太原人,硕士研究生,研究方向为生物化学与分子生物学,E-mail:wujinhua1986@163.com;
  • 基金资助:
    公益性行业(农业)科研专项(201103002);农业部"948"项目(2011-G4)资助

Effects of Artificial Diets on Digestive Enzyme Activities in Loxostege sticticalis

WU Jin-hua1,2, LIU Ai-ping1, XU Lin-bo1, GAO Shu-jing1, HAN Bing2, KANG Ai-guo3, ZHANG Yu-hui3   

  1. 1. Grassland Research Institute, Chinese Academy of Agricultural Sciences, Huhhot, Inner Mongolia 010010, China;
    2. College of Life Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China;
    3. Plant Protection Station of Kangbao County, Kangbao, Hebei Province 076650, China
  • Received:2012-05-16 Revised:2012-09-25 Online:2012-12-15 Published:2012-12-28

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摘要： 通过对经验型筛选和正交试验优化出的饲料配方饲养的草地螟(Loxostege sticticalis)5龄幼虫中肠淀粉酶、蛋白酶、酯酶和脂肪酶4种消化酶进行活性测定。结果表明:饲料HG在蔗糖的添加量稍做改动后,其饲养效果更接近于草地螟天然食料灰菜(Chenopodium album);饲料12的淀粉酶、蛋白酶、酯酶和脂肪酶都与对照组相差较多,蛋白含量、脂类含量与糖类含量需做进一步的修正与试验。另外利用RT-PCR方法,通过研究人工饲料HG, 12及对照组H对草地螟羧酸酯酶基因转录水平的影响,从分子水平快速评价出饲料HG优于饲料12。研究结果为今后的草地螟人工饲养及饲料配方进一步改进提供了理论依据。

关键词: 草地螟, 人工饲料, 消化酶, 羧酸酯酶, RT-PCR

Abstract: This paper studied the bowel amylase, protease, ester enzyme and lipase of Loxostege sticticalis 5-instar larva fed by optimal feed formula which was selected by experience screening and orthogonal test. Results showed that the feed effect of artificial diet HG after adding sucrose was closer to that of Chenopodium album. The amylase, protease, esterase and lipase activities of artificial diet 12 were different from those of control group. In addition, the effects of artificial diet HG and 12 on carboxylesterase gene transcription levels of Loxostege sticticalis were studied using RT-PCR. Artificial diet HG was superior to artificial diet 12. These results provided a theoretical basis for further improvement of Loxostege sticticalis L. artificial feeding and feed formula.

Key words: Loxostege sticticalis, Artificial diets, Digestive enzymes, Carboxylesterase, RT-PCR

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