柳枝稷体外诱导花器官逆转与非逆转穗芽生理特性差异研究

doi:10.11733/j.issn.1007-0435.2017.04.012

草地学报 ›› 2017, Vol. 25 ›› Issue (4): 768-776.DOI: 10.11733/j.issn.1007-0435.2017.04.012

柳枝稷体外诱导花器官逆转与非逆转穗芽生理特性差异研究

王勇锋¹, 李毛¹, 崔桂宾¹, 徐开杰², 孙风丽¹, 张超¹, 刘曙东¹, 奚亚军¹

1. 西北农林科技大学农学院, 陕西杨凌 712100;
2. 中国农业科学院棉花研究所, 河南安阳 455000

收稿日期:2015-11-10 修回日期:2016-12-06 出版日期:2017-08-15 发布日期:2017-11-01
通讯作者: 奚亚军,E-mail:xiyajun11@126.com
作者简介:王勇锋(1989-),男,河南许昌人,博士,主要从事植物发育研究,E-mail:yfw1989@sina.com;李毛(1989-),男,山西运城人,博士,主要从事植物发育研究,E-mail:limao28ban@126.com;
基金资助:
国家自然科学基金（31171607，31371690）资助

Physiological Characteristic Diversity in Reversed and Non-Reversed Spike Buds of Switchgrass

WANG Yong-feng¹, LI Mao¹, CUI Gui-bin¹, XU Kai-Jie², SUN Feng-li¹, ZHANG Chao¹, LIU Shu-dong¹, XI Ya-jun¹

1. College of Agronomy, Northwest A & F University, Yangling, Shaanxi Province 712100, China;
2. Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan Province 455000, China

Received:2015-11-10 Revised:2016-12-06 Online:2017-08-15 Published:2017-11-01

摘要/Abstract

摘要：

柳枝稷（Panicum virgatum L.）是一种短日照开花作物，具有适应性广和生物质产量高等特点，在国际上被广泛认定为模式能源植物。前期研究发现带有1~2 cm长幼穗的柳枝稷茎端组织在穗芽诱导培养基上培养能够通过花器官逆向发育（生殖生长转向营养生长）形成大量再生芽，称为逆转穗芽，而在MS基本培养基上只能通过腋芽形成少量再生芽，称为非逆转穗芽。为探索柳枝稷逆转穗芽形成机制，对逆转穗芽和非逆转穗芽进行了生理学差异研究。结果发现：柳枝稷逆转穗芽具有更高的总糖含量和更高的氮利用效率，但叶绿素含量却较低；逆转穗芽具有更强的抗氧化酶系统（SOD，POD，CAT，PPO）；外源施加细胞分裂素对柳枝稷内源玉米素的合成无显著影响，但对内源吲哚乙酸的合成起到一定的抑制作用，而低水平的吲哚乙酸可能是逆转穗芽诱导起始的关键；脱落酸在逆转穗芽发育的后期阶段起到重要的作用，而赤霉素在其发育的整个时期都行使重要的功能。

关键词: 柳枝稷, 花逆转, 生殖生长, 营养生长, 激素, 生理特性

Abstract:

Swithchgrass(Panicum virgatum L.)is a short-day plant. Due to its wide adaptability and high biomass yield, switchgrass was regarded as the model biofuel plant world widely. Previous study on switchgrass showed that the shoot apexes of tillers at elongation stage 4, which harboring a spike 1~2 cm long, incubated on shoot induction medium would induce flower reversion and finally formed massive regeneration buds, named as reversed spike buds. Correspondingly, shoot apexes incubated on the MS basal medium showed no flower reverse and finally formed little buds, named non-reversed spike buds. To discover the underlying mechanism of the reversed spike bud formation, some physiological indexes of the reversed spike buds and non-reversed spike buds were compared. The results showed that the reversed spike buds had higher sugar content but lower chlorophyll content, and they were more effective in nitrogen utilization; the activity of antioxidant enzyme system (SOD, POD, CAT, PPO) were higher; the exogenous supplementary of cytokinin exhibited little effect on endogenous cytokinin zeatin, however it suppressed the endogenous indoleacetic acid to some extent, which might play an important role in spike buds initiation. Abscisic acid affected spike buds development at the late stage while gibberellin affected spike buds development throughout the whole process.

Key words: Switchgrass, Flower reversion, Vegetative growth, Reproductive growth, Hormone, Physiological characteristic

中图分类号:

Q945.45
S578

王勇锋, 李毛, 崔桂宾, 徐开杰, 孙风丽, 张超, 刘曙东, 奚亚军. 柳枝稷体外诱导花器官逆转与非逆转穗芽生理特性差异研究[J]. 草地学报, 2017, 25(4): 768-776.

WANG Yong-feng, LI Mao, CUI Gui-bin, XU Kai-Jie, SUN Feng-li, ZHANG Chao, LIU Shu-dong, XI Ya-jun. Physiological Characteristic Diversity in Reversed and Non-Reversed Spike Buds of Switchgrass[J]. Acta Agrestia Sinica, 2017, 25(4): 768-776.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://manu40.magtech.com.cn/Jweb_cdxb/CN/10.11733/j.issn.1007-0435.2017.04.012

https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2017/V25/I4/768

参考文献

[1] Kesy J, Maciejewska B, Sowa M, et al. Ethylene and IAA interactions in the inhibition of photoperiodic flower induction of Pharbitis nil[J]. Plant Growth Regulation,2008,55(1):43-50
[2] Balasubramanian S, Sureshkumar S, Lempe J, et al. Potent induction of Arabidopsis thaliana flowering by elevated growth temperature[J]. PLoS Genet,2006,2(7):e106
[3] Sringarm K, Potchanasin P, Sruamsiri P, et al. Floral induction (FI) in longan (Dimocarpus longan, Lour.) trees-The possible participation of endogenous hormones Ⅱ. Low temperature and potassium chlorate effects on hormone concentrations in and their export out of leaves[J]. Scientia Horticulturae,2009,122(2):295-300
[4] Cheruth A J, Kurup S S, Subramaniam S. Variations in Hormones and Antioxidant Status in Relation to Flowering in Early, Mid, and Late Varieties of Date Palm (Phoenix dactylifera) of United Arab Emirates[J]. Scientific World Journal,2015:846104
[5] Farooqi A H A, Shukla Y N, Sharma S, et al. Relationship between Gibberellin and Cytokinin Activity and Flowering in Rosa-Damascena Mill[J]. Plant Growth Regulation,1994,14(2):109-113
[6] Wang K, Tang D, Hong L, et al. DEP and AFO Regulate Reproductive Habit in Rice[J]. Plos Genet,2010,6(1)
[7] Battey N H, Lyndon R F. Reversion of flowering[J]. Botanical Review,1990,56(2):162-189
[8] Okamuro J K, denBoer B G W, Lotys-Prass C, et al. Flowers into shoots:Photo and hormonal control of a meristem identity switch in Arabidopsis[J]. Proc Natl Acad Sci USA,1996,93(24):13831-13836
[9] Tooke F, Ordidge M, Chiurugwi T, et al, Mechanisms and function of flower and inflorescence reversion[J]. Journal of Experimental Botany,2005,56(420):2587-2599
[10] Colasanti J, Yuan Z, and Sundaresan V, The indeterminate gene encodes a zinc finger protein and regulates a leaf-generated signal required for the transition to flowering in maize[J]. Cell,1998,93(4):593-603
[11] Yuan Z, Zhang D. Roles of jasmonate signalling in plant inflorescence and flower development[J]. Current Opinion Plant Biology,2015,27:44-51
[12] D'Aloia M, Bonhomme D, Bouche F, et al, Cytokinin promotes flowering of Arabidopsis via transcriptional activation of the FT paralogue TSF[J]. Plant Journal,2011,65(6):972-979
[13] Gaudinova A, Malbeck J, Dobrev P, et al, Cytokinin, auxin, and abscisic acid dynamics during flower development in white and red currants infected with Blackcurrant reversion virus[J]. Physiological and Molecular Plant Pathology,2008,73(6):119-125
[14] Bransby D. Switchgrass:a Valuable Biomass Crop for Energy[M]. Springer-Verlag,2012:636-636
[15] Martinez-Reyna J M, Vogel K P, Controlled hybridization technique for switchgrass[J]. Crop Science,1998,38(3):876-878
[16] 柴乖强,徐开杰,王勇峰,等, 柳枝稷人工穗芽高效再生体系的建立[J]. 草业学报,2012,21(04):98-104
[17] Washburn C F, Thomas J F. Reversion of flowering in Glycine Max (Fabaceae)[J]. American Journal of Botany,2000,87(10):1425-1438
[18] You X, Wang L, Liang W, et al. Floral reversion mechanism in longan (Dimocarpus longan Lour.) revealed by proteomic and anatomic analyses[J]. Journal of Proteomics,2012,75(4):1099-1118
[19] 张秀丽,李劲涛,杨军,等. 凤仙花花逆转突变体的组织学研究[J]. 园艺学报,2007,34(06):1503-1508
[20] Martínez-Reyna J M, Vogel K P. Incompatibility systems in switchgrass[J]. Crop Science,2002,42(6):1800-1805
[21] Moore K J, Moser L E, Vogel K P, et al. Describing and quantifying growth stages of perennial forage grasses[J]. Agronomy Journal,1991,83(6):1073-1077
[22] Murashige T, Skoog F. A revised medium for rapid growth and bio assays with tobacco tissue cultures[J]. Physiology Plant,1962,15(3):473-497
[23] 张志良,瞿伟菁. 植物生理学试验指导[M]. 第三版.北京:高等教育出版社,2003
[24] 王文平. 植物样品中游离氨基酸总量测定方法的改进[J]. 北京农学院学报,1998,13(03):9-13
[25] 高俊凤. 植物生理学试验指导[M]. 北京:高等教育出版社,2006
[26] 白素兰,谢中稳,刘永胜,等, 植物的成花逆转[J]. 植物生理学通讯,2000,36(03):252-257
[27] Young T E, Giesler-Lee J, Gallie D R, Senescence-induced expression of cytokinin reverses pistil abortion during maize flower development[J]. Plant Journal,2004,38(6):910-922
[28] Cai Q, Yuan Z, Chen M J, et al, Jasmonic acid regulates spikelet development in rice[J]. Nature Communications,2014,5:3476
[29] Ampomah-Dwamena C, Morris B A, Sutherland P, et al. Down-regulation of TM29, a tomato SEPALLATA homolog, causes parthenocarpic fruit development and floral reversion[J]. Plant Physiology,2002,130(2):605-617

柳枝稷体外诱导花器官逆转与非逆转穗芽生理特性差异研究

Physiological Characteristic Diversity in Reversed and Non-Reversed Spike Buds of Switchgrass

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	杜建雄, 任尉香, 袁涓文, 黄慧琼, 刘杰, 肖玉. 汞胁迫对4个草坪草、牧草品种幼苗生长和生理的影响[J]. 草地学报, 2021, 29(8): 1712-1718.
[2]	朱琨, 刘丽杰, 周璐, 李珊珊, 范震宇, 吴纪一, 尼尼. 外源激素对染白粉菌草地早熟禾叶片膜透性及表面结构的影响[J]. 草地学报, 2021, 29(6): 1210-1216.
[3]	王文静, 麻冬梅, 赵丽娟, 马巧利. 2,4-表油菜素内酯对盐胁迫下紫花苜蓿生理指标及根系离子积累的影响[J]. 草地学报, 2021, 29(6): 1363-1368.
[4]	王燕, 赵晓晓, 王伟伟. 柳枝稷PvJAZ1基因的克隆、表达特性与亚细胞定位[J]. 草地学报, 2021, 29(2): 234-243.
[5]	陈斌, 薛晴, 李子葳, 杨宇佳, 杨小梅, 薄杉, 李强, 何淼. 光强对4种鸭跖草科植物叶片生理特性和超微结构的影响[J]. 草地学报, 2021, 29(2): 281-292.
[6]	贾志锋, 马祥, 琚泽亮, 刘凯强, 赵桂琴. 施氮量和播种量对燕麦光合特性、激素含量及种子产量的影响[J]. 草地学报, 2021, 29(2): 293-302.
[7]	张力天, 刘炜, 刘德梅, 董瑞珍, 王晓丽, 张敏, 仁增曲扎, 边巴普尺, 杨时海, 马玉寿. 青藏高原白草生殖生长期植株器官生物量分配特征[J]. 草地学报, 2021, 29(12): 2694-2702.
[8]	余淑艳, 周燕飞, 黄薇, 王星, 高雪芹, 伏兵哲. 干旱、盐及旱盐复合胁迫对沙芦草光合和生理特性的影响[J]. 草地学报, 2021, 29(11): 2399-2406.
[9]	李萱, 刘博文, 高双红, 游翔凯, 龙思, 李尤月, 刘茜, 张士军, 许岳飞. 外源硫化氢对不同弱光下高羊茅形态和生理指标的影响[J]. 草地学报, 2021, 29(11): 2435-2441.
[10]	高双红, 李媛英, 刘博文, 龙思, 刘铁芫, 许岳飞. H₂S对弱光胁迫下高羊茅幼苗生理特性和内源激素的影响[J]. 草地学报, 2021, 29(10): 2233-2239.
[11]	王晓雪, 李越, 张斌, 贾举庆, 冯美臣, 张美俊, 杨武德. 干旱胁迫及复水对燕麦根系生长及生理特性的影响[J]. 草地学报, 2020, 28(6): 1588-1596.
[12]	陈爱萍, 隋晓青, 王玉祥, 靳瑰丽, 王堃, 安沙舟. 干旱胁迫及复水对伊犁绢蒿幼苗生长及生理特性的影响[J]. 草地学报, 2020, 28(5): 1216-1225.
[13]	马雪瑶, 隋晓青, 李俊明, 张博. 叶面喷施GA₃对紫花苜蓿花荚内源激素及种子产量构成的影响[J]. 草地学报, 2020, 28(5): 1294-1300.
[14]	徐美蓉, 李敏权, 曹素芳, 蒋晶晶, 李雪萍, 孙新荣, 陈爱昌, 李继平, 漆永红. 根腐病对党参细胞结构和生理特性的影响[J]. 草地学报, 2020, 28(4): 915-922.
[15]	杨航, 祁娟, 李玉英, 刘艳君, 吴召林, 金鑫. 外源激素与磷素配施对老芒麦生长特性及营养品质的影响[J]. 草地学报, 2020, 28(4): 1015-1023.