UV-B辐射对不同生态型垂穗披碱草幼苗生长和抗氧化酶系统的影响

doi:10.11733/j.issn.1007-0435.2019.04.014

草地学报 ›› 2019, Vol. 27 ›› Issue (4): 898-905.DOI: 10.11733/j.issn.1007-0435.2019.04.014

UV-B辐射对不同生态型垂穗披碱草幼苗生长和抗氧化酶系统的影响

刘茜¹, 刘博文¹, 王洋¹, 奥宝¹, 田沛鑫¹, 文素芸¹, 张旭虎¹, 苗彦军², 许岳飞¹

1. 西北农林科技大学草业与草原学院, 陕西杨凌 712100;
2. 西藏农牧学院植物科学学院, 西藏林芝 860000

收稿日期:2018-09-29 修回日期:2019-03-22 出版日期:2019-08-15 发布日期:2019-09-26
通讯作者: 许岳飞
作者简介:刘茜(1996-),女,汉族,陕西商洛人,硕士研究生,主要从事牧草、草坪草逆境生物学研究,E-mail:liuqian9577@nwsuaf.edu.cn
基金资助:
陕西省畜牧技术推广项目（20181214000004）；中央高校基本科研业务费专项资金资助（2452019080）资助

Effects of UV-B Radiation on the Growth and Antioxidative Enzymes System of Two Eco-types Elymus nutans Seedlings

LIU Qian¹, LIU Bo-wen¹, WANG Yang¹, AO Bao¹, TIAN Pei-xin¹, WEN Su-yun¹, ZHANG Xu-hu¹, MIAO Yan-jun², XU Yue-fei¹

1. College of Grassland Agriculture, Northwest A & F University, Yangling, Shaanxi Province 712100, China;
2. College of Plant Science, Tibet Agriculture and Animal Husbandry College, Linzhi, Tibet 860000, China

Received:2018-09-29 Revised:2019-03-22 Online:2019-08-15 Published:2019-09-26

摘要/Abstract

摘要： UV-B辐射是影响植物生长的外界限制因子之一。本文研究了UV-B辐射对2个生态型垂穗披碱草（Elymus nutans）（商用型正道，ZD；西藏野生型申扎，XZ）幼苗的生长和抗氧化系统的影响。结果表明：UV-B辐射处理后，二者的生物量和叶绿素含量受到不同程度地抑制，ZD地下生物量和根茎比受UV-B辐射抑制作用显著；处理后二者的丙二醛含量显著提高，且XZ的丙二醛含量显著低于ZD；UV-B还显著增加了垂穗披碱草幼苗的可溶性蛋白含量，ZD的脂氧合酶活性也有显著提高，但XZ并无显著变化；另外，幼苗的超氧化物歧化酶和过氧化物酶活性受UV-B辐射影响均不显著，但XZ的超氧化物歧化酶活性显著高于ZD，而过氧化物酶活性显著低于ZD；二者的过氧化氢酶和谷胱甘肽还原酶活性受UV-B辐射影响显著，但生态型间无显著差异。综上，XZ受UV-B辐射影响小于ZD，对UV-B辐射有较高抗性，这可能与其稳定的脂氧合酶活性和较高的超氧化物歧化酶活性等有关。

关键词: UV-B辐射, 垂穗披碱草, 抗氧化酶系统, 西藏, 生态型

Abstract: UV-B radiation is one of the limiting factors affecting plant growth. The effects of UV-B radiation on the growth and antioxidative enzymes system of two ecological types of Elymus nutans (Commercial type from Beijing zhengdao,ZD;Tibetan wild-type from Xainza,XZ) seedlings were studied using sand culture method. The results showed that the biomass and chlorophyll content of two eco-types of E. nutans were inhibited to varying degrees after UV-B radiation treatment,and the growth of ZD's below-ground biomass and root-shoot index was significantly inhibited. UV-B radiation treatment significantly increased the malondialdehyde content of E. nutans,and the malondialdehyde content of XZ was significantly lower than ZD. And,UV-B radiation significantly increased the soluble protein content of E. nutans, and lipoxygenase activity of ZD increased significantly,but there was no significant difference between the UV-B radiation treatment and the control in XZ. The superoxide dismutase and peroxidase activities of two eco-types E. nutans didn't not change significantly after UV-B radiation treatment. The activity of superoxide dismutase in XZ was significantly higher than that in ZD,while the activity of peroxidase was significantly lower than that in ZD. However,the activities of catalase and glutathione reductase were significantly affected by UV-B radiation,but there was no significant difference between the two eco-types. It is reasonably to infer that XZ is less affected by UV-B radiation than ZD and has higher resistance to UV-B radiation,which may be related to its stable lipoxygenase activity and higher superoxide dismutase activity.

Key words: UV-B radiation, Elymus nutans, Antioxidative enzymes system, Tibet, Ecological type

中图分类号:

Q945.78

刘茜, 刘博文, 王洋, 奥宝, 田沛鑫, 文素芸, 张旭虎, 苗彦军, 许岳飞. UV-B辐射对不同生态型垂穗披碱草幼苗生长和抗氧化酶系统的影响[J]. 草地学报, 2019, 27(4): 898-905.

LIU Qian, LIU Bo-wen, WANG Yang, AO Bao, TIAN Pei-xin, WEN Su-yun, ZHANG Xu-hu, MIAO Yan-jun, XU Yue-fei. Effects of UV-B Radiation on the Growth and Antioxidative Enzymes System of Two Eco-types Elymus nutans Seedlings[J]. Acta Agrestia Sinica, 2019, 27(4): 898-905.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://manu40.magtech.com.cn/Jweb_cdxb/CN/10.11733/j.issn.1007-0435.2019.04.014

https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2019/V27/I4/898

参考文献

[1] Norsang G,Kocbach L,Tsoja W,et al. Ground-based measurements and modeling of solar UV-B radiation in Lhasa,Tibet[J]. Atmospheric Environment,2009,43(8):1498-1502
[2] Norsang G,Ma L,Dahlback A,et al. The vitamin D status among Tibetans[J]. Photochemistry & Photobiology,2010,85(4):1028-1031
[3] 孙金伟,任斐鹏,任亮,等. UV-B辐射对植物生理生态特征的影响研究进展[J]. 长江科学院院报,2015,32(3):107-111
[4] 李永鑫. 西藏地表生物有效紫外辐射观测研究[D]. 拉萨:西藏大学,2015:3
[5] 卢宝荣. 披碱草属与大麦属系统关系的研究[J]. 植物分类学报,1997,35(3):193-207
[6] 陈智华,苗佳敏,钟金城,等. 野生垂穗披碱草种质遗传多样性的SRAP研究[J]. 草业学报,2009,18(5):192-200
[7] 马啸,周永红,于海清,等. 野生垂穗披碱草种质的醇溶蛋白遗传多样性分析[J]. 遗传,2006,28(6):699-706
[8] Zhou X,Li C,Zhang S. Effects of ambient ultraviolet-B on ecophysiological characteristics and forage quality of Elymus nutans in the Tibetan alpine grasslands[J]. Grassland Science,2014,60(2):98-103
[9] Yun H,Lim S,Kim Y X,et al. Diurnal Changes in C,N Metabolism and Response of Rice Seedlings to UV-B Radiation[J]. Journal of Plant Physiology,2018,228:66-74
[10] Zhang X,Ding X,Ji Y,et al. Measurement of metabolite variations and analysis of related gene expression in Chinese liquorice (Glycyrrhiza uralensis) plants under UV-B irradiation[J]. Scientific Reports,2018,8(1):6144
[11] Januškaitiene·I,Kaciene·G. The effect of foliar spray fertilizers on the tolerance of Hordeum vulgare to UV-B radiation and drought stress[J]. Cereal Research Communications,2017,45(3):1-11
[12] 侯常伟,白涛,王忆,等. UV-B辐射对光核桃光合作用和内源激素水平的影响[J]. 中国农学通报,2012,28(22):184-189
[13] Allen D J,Nogues S,Baker N R. Ozone depletion and increased UV-B radiation:is there a real threat to photosynthesis[J]. Journal of Experimental Botany,1998,49(328):1775-1788
[14] Fisher R A,Tanabe S,Buxton D B,et al. Ultraviolet-B irradiation-induced freezing tolerance in relation to antioxidant system in winter wheat (Triticum aestivum L.) leaves[J]. Environmental & Experimental Botany,2007,60(3):300-307
[15] 许大全. 叶绿素含量的测定及其应用中的几个问题[J]. 植物生理学报,2009,45(9):896-898
[16] Li Y,Zhao H,Duan B,et al. Effect of drought and ABA on growth,photosynthesis and antioxidant system of Cotinus coggygria,seedlings under two different light conditions[J]. Environmental & Experimental Botany,2011,71(1):107-113
[17] Elstner E F,Heupel A. Inhibition of nitrite formation from hydroxylammonium chloride:a simple assay for superoxide dismutase.[J]. Analytical Biochemistry,1976,70(2):616-620
[18] Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical Biochemistry,1976,72(s1-2):248-254
[19] 石胜尧,张延坤,郭大发,等. 大豆脂肪氧化酶活性的测定[J]. 营养学报,1996(3):354-357
[20] Beauchamp C,Fridovich I. Superoxide dismutase:Improved assays and an assay applicable to acrylamide gels[J]. Analytical Biochemistry,1971,44(1):276-287
[21] Cakmak I,Marschner H. Magnesium deficiency and high light intensity enhance activities of superoxide dismutase,ascorbate peroxidase,and glutathione reductase in bean leaves[J]. Plant Physiology,1992,98(4):1222-1227
[22] Upadhyaya A,Sankhla D,Davis T D,et al. Effect of paclobutrazol on the activities of some enzymes of activated oxygen metabolism and lipid peroxidation in senescing soybean leaves[J]. Journal of Plant Physiology,1985,21(5):453-461
[23] Foyer C H,Halliwell B. The presence of glutathione and glutathione reductase in chloroplasts:A proposed role in ascorbic acid metabolism[J]. Planta,1976,133(1):21-25
[24] Mazza C A,Giménez P I,Kantolic A G,et al. Beneficial effects of solar UV-B radiation on soybean yield mediated by reduced insect herbivory under field conditions[J]. Physiologia Plantarum,2013,147(3):307-315
[25] 张玉红,陈路瑶,刘彤,等. 增补UV-B辐射对药用植物黄檗幼苗生长及光合生理影响[J]. 中国农学通报,2018,34(4):76-82
[26] He J,Huang L K,Chou W S. Effects of supplementary ultraviolet-B radiation on rice and pea plants[J]. Functional Plant Biology,1993,20(2):129-142
[27] Ranjbarfordoei A,Samson R,Damme P V. Photosynthesis performance in sweet almond (Prunus dulcis (Mill) D. Webb) exposed to supplemental UV-B radiation[J]. Photosynthetica,2011,49(1):107-111
[28] 刘智微,钟小仙,沈益新. 海盐胁迫对苏牧2号象草抗氧化酶活性和MDA含量的影响[J]. 草地学报,2011,33(1):24-29
[29] 刘碧容,甄畅迪,萧洪东,等. 硼对草坪草超氧化物歧化酶活性,超氧阴离子产生速率和丙二醛含量的影响[J]. 华中农业大学学报,2008,7(3):378-381
[30] 刘鹏,杨玉爱. 钼,硼对大豆叶片膜脂过氧化及体内保护系统的影响[J]. 植物学报,2000,42(5):461-466
[31] Di V A,Salucci M L,Van Z G,et al. Structure-to-Function Relationship of Mini-Lipoxygenase,a 60-kDa Fragment of Soybean Lipoxygenase-1 with Lower Stability but Higher Enzymatic Activity[J]. Journal of Biological Chemistry,2003,278(20):18281-18288
[32] 胡廷章,胡宗利,屈霄霄,等. 植物脂肪氧化酶的研究进展[J]. 生物工程学报,2009,25(1):1-9
[33] Porta H,Ruedabenítez P,Campos F,et al. Analysis of Lipoxygenase mRNA Accumulation in the Common Bean (Phaseolus vulgaris L.) during Development and under Stress Conditions[J]. Plant & Cell Physiology,1999,40(8):850-858
[34] Mittler R,Vanderauwera S,Gollery M,et al. Reactive oxygen gene network of plants[J]. Trends in Plant Science,2004,9(10):490-498
[35] Kataria S,Jajoo A,Guruprasad K N. Impact of increasing Ultraviolet-B (UV-B) radiation on photosynthetic processes.[J]. Journal of Photochemistry & Photobiology Biology,2014,137(8):55-66
[36] 褚润,陈年来,韩国君,等. UV-B辐射增强对芦苇生长及生理特性的影响[J]. 环境科学学报,2018,38(05):389-396
[37] 吴永波,薛建辉. UV-B辐射增强对植物影响的研究进展[J]. 世界林业研究,2004,17(3):29-31

UV-B辐射对不同生态型垂穗披碱草幼苗生长和抗氧化酶系统的影响

Effects of UV-B Radiation on the Growth and Antioxidative Enzymes System of Two Eco-types Elymus nutans Seedlings

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	宋建超, 鱼小军, 魏孔涛, 汪鹏斌, 童永尚, 李珍, 贺有龙, 汪海波. 施氮对高寒区垂穗披碱草饲草生产性能及营养品质的影响[J]. 草地学报, 2021, 29(7): 1555-1564.
[2]	张力天, 刘德梅, 刘博, 杨时海, 王彦龙, 王晓丽, 马玉寿. 西藏自治区日喀则市白草核型分析[J]. 草地学报, 2021, 29(7): 1589-1593.
[3]	其美拉姆, 普布卓玛, 崔彤彤, 张新飞, 姜惠娜, 付娟娟, 呼天明. 外源钙对低温胁迫下西藏野生垂穗披碱草生理及相关基因表达的影响[J]. 草地学报, 2021, 29(5): 919-928.
[4]	秦金萍, 刘颖, 马玉寿, 王彦龙, 李世雄. 返青期休牧对退化高寒草地植被特征及优势种光合生理的影响[J]. 草地学报, 2021, 29(5): 1034-1042.
[5]	岳丽楠, 师尚礼, 祁娟, 杨培志, 刘刚, 刘文辉, 张英俊, 荆晶莹. 免耕补播对北方退化草地生产力及营养品质的影响[J]. 草地学报, 2021, 29(11): 2583-2590.
[6]	姜惠娜, 敬松, 李晗玉, 佘木子, 张新飞, 呼天明, 付娟娟, 苗彦军. 西藏野生垂穗披碱草EnPLA1基因克隆与表达分析[J]. 草地学报, 2021, 29(10): 2141-2148.
[7]	姚泽英, 李军, 宋连昭, 刘春阳, 姚泽军, 刘贵河. 垂穗披碱草与紫花苜蓿不同时期交叉混播效果研究[J]. 草地学报, 2020, 28(5): 1454-1459.
[8]	姚泽英, 李军, 宋连昭, 刘春阳, 姚泽军, 刘贵河. 张家口坝上地区豆-禾牧草混播效果研究[J]. 草地学报, 2020, 28(4): 1076-1082.
[9]	普布卓玛, 其美拉姆, 李丹丹, 多吉顿珠, 张靖, 孙运府, 苗彦军, 付娟娟, 呼天明. 外源脯氨酸对低温胁迫下西藏野生垂穗披碱草幼苗生长和抗氧化酶相关基因表达的影响[J]. 草地学报, 2020, 28(3): 589-596.
[10]	赵方媛, 杜文华, 曲广鹏, 钟志明, 刘昭明, 田新会. 黑麦新品系C32在西藏牧区的生产性能和营养价值研究[J]. 草地学报, 2020, 28(1): 279-284.
[11]	郭斌, 王珊, 陈超, 王明田, 李婷婷. 气候变化背景下川西北高原多年生垂穗披碱草种植适生区分布预测[J]. 草地学报, 2019, 27(6): 1596-1606.
[12]	潘影, 张燕杰, 武俊喜, 张宪洲, 余成群. 基于遥感和无人机数据的草地NDVI影响因子多尺度分析[J]. 草地学报, 2019, 27(6): 1766-1773.
[13]	王传旗, 付娟娟, 刘敏洁, 周青平, 苗彦军. 垂穗披碱草对Cu²⁺，Zn²⁺胁迫的生理响应[J]. 草地学报, 2019, 27(6): 1781-1785.
[14]	张光雨, 沈振西, 邵小明, 付刚, 马和平. 西藏当雄10个引进燕麦品种的生产性能和营养品质比较[J]. 草地学报, 2019, 27(4): 1083-1089.
[15]	普布卓玛, 罗艺岚, 高金柱, 孙鹏越, 赵东豪, 苗彦军, 付娟娟, 呼天明. 2,4表-油菜素内酯对低温胁迫下西藏野生垂穗披碱草幼苗抗氧化保护和渗透调节的影响[J]. 草地学报, 2019, 27(3): 547-552.