草地学报

草地学报 ›› 2019, Vol. 27 ›› Issue (5): 1237-1242.DOI: 10.11733/j.issn.1007-0435.2019.05.016

• 研究论文 • 上一篇    下一篇

盐爪爪种子萌发期抗盐性研究

马彦军, 祝小娟, 何瑞雪   

  1. 甘肃农业大学林学院, 甘肃 兰州 730070
  • 收稿日期:2019-06-05 修回日期:2019-08-19 出版日期:2019-10-15 发布日期:2019-11-09
  • 作者简介:马彦军(1975-),男,甘肃平凉市人,回族,博士,副教授,研究方向为植物种质资源调查收集、保存与研究,E-mail:mazhjie@126.com
  • 基金资助:
    甘肃农业大学青年研究生指导教师扶持基金项目（GAU-QNDS-201712）；甘肃省GEF/OP12三期项目专题研究项目（GS-GEF/OP12-02）共同资助

Study on Salt Resistance of Kalidium foliatum Seeds at Germination Stage

MA Yan-jun, ZHU Xiao-jun, HE Rui-xue   

  1. Forestry College, Gansu Agriculture University, Lanzhou, Gansu Province 730070
  • Received:2019-06-05 Revised:2019-08-19 Online:2019-10-15 Published:2019-11-09

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摘要： 为了解盐爪爪（Kalidium foliatum）种子分别在NaCl和Na2SO4胁迫下种子萌发期的抗盐性，本试验采用发芽盒进行种子萌发出苗试验，测定盐爪爪种子在不同浓度（0，50，100，200，300，400 mmol·L-1）NaCl和Na2SO4胁迫下的萌发率、发芽势、发芽指数、根茎比以及幼苗可溶性蛋白、抗氧化酶活性。结果表明：盐爪爪种子在NaCl和Na2SO4浓度均为50 mmol·L-1时萌发率最高，当盐浓度大于100 mmol·L-1时萌发率开始下降；盐爪爪种子发芽势随盐浓度增加而下降，幼苗根茎比以及发芽指数随盐浓度增加呈现先升后降趋势。在NaCl和Na2SO4胁迫下，盐爪爪幼苗体内可溶性蛋白含量随盐浓度增加呈上升趋势；超氧化物歧化酶（Superoxide dismutase，SOD）、过氧化物酶（Peroxidase，POD）酶活性呈现出先上升后下降趋势，过氧化氢酶（Catalase，CAT）酶活性呈下降趋势。综合分析表明，盐爪爪种子在盐浓度小于100 mmol·L-1时能正常萌发出苗；盐爪爪幼苗可通过增加体内可溶性蛋白含量、SOD和POD酶活性来抵御盐对其产生的危害。

关键词: 盐爪爪, 种子萌发, 盐胁迫, 抗氧化酶

Abstract: In order to clarify the salt resistance of Kalidium foliatum seeds at germination stage,seeds germination tests were carried out under stress of NaCl and Na2SO4 with concentration of 0,50,100,300,400 mmol·L-1, respectively,in a germination box. The germination rate,germination potential,germination index,root-stem ratio,soluble protein,and antioxidase activities were measured and analyzed. The results showed that the germination rate of Kalidium foliatum was the highest when the concentrations of NaCl and Na2SO4 were 50 mmol·L-1. The germination rate decreased when the concentrations of NaCl and Na2SO4 were 100 mmol·L-1. The germination potential decreased with the increase of salt concentration,and the root-stem ratio and germination index increased firstly and then decreased with the increase of salt concentration. Under the stress of NaCl and Na2SO4,the content of soluble protein of Kalidium foliatum seedlings increased with the increase of salt concentration,the activities of superoxide dismutase (SOD)and peroxidase(POD) increased firstly and then decreased,and the activity of catalase (CAT) showed a decreasing trend. Our experiments showed that the Kalidium foliatum seeds could germinate normally under salt stress of less than 100 mmol·L-1. The Kalidium foliatum seedlings can resist the hazard of salty environment by increasing the internal soluble protein content and the SOD and POD activities.

Key words: Kalidium foliatum, Seed germination, Salt stress, Antioxidant enzymes

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