草地学报

草地学报 ›› 2019, Vol. 27 ›› Issue (6): 1477-1485.DOI: 10.11733/j.issn.1007-0435.2019.06.003

• 研究论文 • 上一篇    下一篇

利用SSR标记对不同耐盐紫花苜蓿遗传多样性分析

麻冬梅, 张喜斌, 黄婷, 王文静, 赵丽娟, 马巧利   

  1. 宁夏大学西北土地退化与生态系统恢复省部共建国家重点实验室培育基地/西北退化生态系统恢复与重建教育部重点实验室/宁夏大学生命科学学院/宁夏大学农学院, 宁夏 银川 750021
  • 收稿日期:2019-10-16 修回日期:2019-10-30 出版日期:2019-12-15 发布日期:2019-12-31
  • 作者简介:麻冬梅(1978-),女,宁夏贺兰县人,博士,副教授,现主要从事植物抗逆生理及分子生物学等研究工作,E-mail:ma_dm@nxu.edu.cn
  • 基金资助:
    国家自然科学基金项目（31760698）；宁夏重点研发项目（2019BBF02022-04）；宁夏高等学校一流学科建设（生态学）资助项目（NXYLXK2017B06）资助

Genetic Diversity Analysis of 10 Salt Tolerant Alfalfa

MA Dong-mei, ZHANG Xi-bin, HUANG Ting, WANG Weng-jing, ZHAO Li-juan, MA Qiao-li   

  1. Breeding Base for State Key Laboratory of Land Degradation and Ecological Restoration in Northwest China/Key Laboratory for Restoration and Reconstruction of Degraded Ecosystem in Northwest China of Ministry of Education/Key Laboratory for Restoration and Reconstruction of Degraded Ecosystem in Northwest China of Ministry of Education/Agricultural College of Ningxia University/Life Science College of Ningxia University, Yinchuan, Ningxia 750021, China
  • Received:2019-10-16 Revised:2019-10-30 Online:2019-12-15 Published:2019-12-31

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摘要： 为了了解不同耐盐紫花苜蓿（Medicago sativa L.）的遗传多样性以及对耐盐紫花苜蓿品种改良，本试验筛选出9对简单重复序列（Simple Sequence Repeats，SSR）引物对10份耐盐、敏盐材料进行SSR多态性分析。并利用MEGA6.0软件对其进行了聚类分析。结果表明：10份紫花苜蓿品种中平均多态位点百分率为47.1%~71.8%；平均有效等位基因观察数与等位基因观察数的比为0.680~0.890；平均杂合度为0.759~0.828；平均香农指数为1.753~1.976；平均多态信息含量为0.735~0.811；遗传分化系数为0.100。利用IBM SPSS Statistics 19软件进行综合评价，根据综合评价值对10个紫花苜蓿品种内的遗传变异程度大小排序，排列顺序为： "巨能" > "骑士T" > "巨能7号" > "Asi" > "兴平" > "阿迪娜" > "秘鲁" > "抗旱15" > "草原3号" > "国产苜蓿"；利用MEGA6.0软件对Nei遗传距离进行UPGMA聚类分析可知：5份敏盐材料始终聚为一类；"巨能"与"巨能7号"聚为一类；"阿迪娜"和"抗旱15"又聚为一类，说明他们之间有较近的亲缘关系。

关键词: 紫花苜蓿, SSR分子标记, 遗传多样性

Abstract: In order to understand the genetic diversity of different salt tolerant alfalfa varieties and improve their breeding. In this experiment,9 pairs of SSR primers were selected to analyze SSR polymorphism of 10 salt tolerant and salt sensitive materials,and cluster analysis was carried out by using mega6.0 software. The results showed that the average percentage of polymorphic loci in 10 Alfalfa varieties was 47.1%~71.8%;The ratio of mean effective allele observation to allele observation was 0.680~0.890;The average heterozygosity was 0.759~0.828;The average Shannon index is 1.753~1.976;The average polymorphism was 0.735~0.811;Genetic differentiation coefficient (GST) was 0.100;Principal component analysis (PCA) and membership function were used to evaluate the average polymorphic locus percentage,average heterozygosity,ratio of average effective allele observation to allele observation,average Shannon index and average polymorphic information content of 10 Alfalfa Cultivars by IBM SPSS Statistics 19 software. According to the comprehensive evaluation value (D value),the genetic variation of 10 Alfalfa varieties was ranked:Magnum > KnightT > MagnumⅦ > Asi > Xingping > Adrenalin > Peru > Kanghan15 > CaoyuanNo.3 > Domestic alfalfa;MEGA 6.0 software was used to analyze the Nei genetic distance by UPGMA cluster analysis. Five sensitive salt materials were always clustered into one group;MagnumⅦ was clustered into one group;Adrenalin and Drought Resistance 15 were clustered into one group,indicating that they had close relationship.

Key words: Alfalfa, SSR Molecular Marker, Genetic Diversity

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