低温胁迫下不同青海野生草地早熟禾的转录组比较分析

doi:10.11733/j.issn.1007-0435.2020.02.003

草地学报 ›› 2020, Vol. 28 ›› Issue (2): 305-318.DOI: 10.11733/j.issn.1007-0435.2020.02.003

低温胁迫下不同青海野生草地早熟禾的转录组比较分析

赵春旭¹, 马祥², 董文科¹, 牛奎举¹, 张然¹, 马晖玲¹

1. 甘肃农业大学草业学院, 草业生态系统教育部重点实验室, 甘肃省草业工程实验室, 中-美草地畜牧业可持续发展研究中心, 甘肃兰州 730070;
2. 青海省青藏高原优良牧草种质资源利用重点实验室, 青海省畜牧兽医科学院, 青海大学, 青海西宁 810016

收稿日期:2019-12-22 修回日期:2020-02-13 出版日期:2020-04-15 发布日期:2020-04-11
通讯作者: 马晖玲
作者简介:赵春旭(1985-),男,甘肃兰州人,博士研究生,主要从事牧草及草坪草种质资源研究,E-mail:zhaocx@gsau.edu.cn
基金资助:
青海省科技厅重点实验室发展专项“青海省青藏高原优良牧草种质资源利用重点实验室”（2020-ZJ-Y03）资助

Transcriptome Comparative Analysis of Different Qinghai Wild Poa pratensis Under Low Temperature Stress

ZHAO Chun-xu¹, MA Xiang², Dong Wen-ke¹, Niu Kui-ju¹, Zhang Ran¹, MA Hui-ling¹

1. College of Pratacultural Science, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem, Ministry of Education, Pratacultural Engineering Laboratory of Gansu Province, Sino-U. S. Center for Grassland Ecosystem Sustainability, Lanzhou, Gansu Province 730070, China;
2. Key Laboratory of Superior Forage Germplasm in the Qinghai-Tibetan Plateau, Qinghai Academy of Animal Science and Veterinary Medicine, Qinghai University, Xining, Qinghai Province 810016, China

Received:2019-12-22 Revised:2020-02-13 Online:2020-04-15 Published:2020-04-11

摘要/Abstract

摘要： 为探究青海野生草地早熟禾（Poa pratensis）低温胁迫下的分子应答机制，本试验以青海省野生草地早熟禾耐寒材料‘10-122’和低温敏感材料‘09-126’为研究对象，采用高通量Illumina Hiseq测序技术分析了在低温胁迫与常温对照间的转录组差异。结果表明：‘10-122’共有31 943个差异表达基因，其中，17 088个差异表达基因上调表达，14 855个差异表达基因下调表达；‘09-126’共有25 905个差异表达基因，其中，13 513个差异表达基因上调表达，12 392个差异表达基因下调表达；对2种材料进行差异表达基因富集分析，得出差异表达基因低温胁迫下在光合作用、氧化还原反应过程、碳水化合物代谢、细胞膜系统、转运蛋白以及次生物代谢中富集显著；此外，一些钙信号调节、激素代谢和信号传导、抗氧化系统、碳水化合物代谢等通路的基因仅在耐寒型种质材料‘10-122’上调表达，可作为潜在的抗寒基因，如CML、CPK、CALM、DHAR、GST、NCED、SNRK2、BSK、CKX、BIN2、ARF和PEK等。

关键词: 低温胁迫, 野生草地早熟禾, 转录组, 差异表达基因

Abstract: In order to explore the molecular response mechanism to low temperature stress of Qinghai wild Poa pratensis,this experiment took cold-resistant germplasm material ‘10-122’ and low-temperature sensitive germplasm material ‘09-126’ as research objects and analyzed transcriptome differences between low temperature stress and normal temperature controls with high-throughput Illumina Hiseq sequencing technology. The results showed that there were 31 943 differentially expressed genes detected in ‘10-122’,in which 17088 differentially expressed genes were up-regulated and 14855 differentially expressed genes were down-regulated. There were 25 905 differentially expressed genes detected in ‘09-126’,in which 13 513 differentially expressed genes were up-regulated and 12 392 differentially expressed genes were down-regulated. The enrichment analysis of differentially expressed genes on two germplasm materials showed that the differentially expressed genes were significantly enriched in photosynthesis,oxidation-reduction,carbohydrate metabolism,cell membrane system,transporter protein,and secondary metabolism under low temperature stress. Additionally,a number of DEGs involved in Ca²⁺ signaling,plant hormone biosynthesis and signaling transduction,antioxidant system and carbohydrate metabolism pathways were specific up-regulated in cold-resistant genotype ‘10-122’. Therefore,these DEGs including CML、CPK、CALM、DHAR、GST、NCED、SNRK2、BSK、CKX、BIN2、ARF were identified as potential genes associated with the cold resistance of ‘10-122’ genotype.

Key words: Low-temperature stress, wild Poa pratensis, Transcriptome, Differentially expressed genes

中图分类号:

Q945.78

赵春旭, 马祥, 董文科, 牛奎举, 张然, 马晖玲. 低温胁迫下不同青海野生草地早熟禾的转录组比较分析[J]. 草地学报, 2020, 28(2): 305-318.

ZHAO Chun-xu, MA Xiang, Dong Wen-ke, Niu Kui-ju, Zhang Ran, MA Hui-ling. Transcriptome Comparative Analysis of Different Qinghai Wild Poa pratensis Under Low Temperature Stress[J]. Acta Agrestia Sinica, 2020, 28(2): 305-318.

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低温胁迫下不同青海野生草地早熟禾的转录组比较分析

Transcriptome Comparative Analysis of Different Qinghai Wild Poa pratensis Under Low Temperature Stress

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