青海野生草地早熟禾响应干旱胁迫的代谢通路及转录调控分析

doi:10.11733/j.issn.1007-0435.2020.06.003

草地学报 ›› 2020, Vol. 28 ›› Issue (6): 1508-1518.DOI: 10.11733/j.issn.1007-0435.2020.06.003

青海野生草地早熟禾响应干旱胁迫的代谢通路及转录调控分析

张然¹, 马祥², 朱瑞婷¹, 牛奎举¹, 赵春旭¹, 马晖玲¹

1. 甘肃农业大学草业学院, 草业生态系统教育部重点实验室, 甘肃省草业工程实验室, 中-美草地畜牧业可持续发展研究中心, 甘肃兰州 730070;
2. 青海省青藏高原优良牧草种质资源利用重点实验室, 青海省畜牧兽医科学院, 青海大学, 青海西宁 810016

收稿日期:2020-06-30 修回日期:2020-08-04 出版日期:2020-12-15 发布日期:2020-12-02
通讯作者: 马晖玲
作者简介:张然(1995-),女,山西绛县人,博士研究生,主要从事草坪生物学研究,E-mail:912459402@qq.com
基金资助:
青海省牧科院基本科研业务费自主选题资助项目“施肥和播期互作对燕麦产量及土壤理化性质的影响研究”（MKY-2019-02）；青海省科技计划项目“青海省青藏高原优良牧草种质资源利用重点实验室”（2020-ZJ-Y03）；国家地区自然基金“基于生态化学计量特征学的青海扁茎早熟禾种子生产生活史繁殖策略调控研究”（31760711）资助

Metabolic Pathway and Transcriptional Regulation of Qinghai Wild Poa pratensis in Response to Drought Stress

ZHANG Ran¹, MA Xiang², ZHU Rui-ting¹, NIU Kui-ju¹, ZHAO Chun-xu¹, MA Hui-ling¹

1. College of Pratacultural Science, Gansu Agricultural University, Key Laboratory of Grassland Ecosystem, Ministry of Education, Pratacultural Engineering Laboratory of Gansu Province, Sino-U. S. Center for Grassland Ecosystem Sustainability, Lanzhou, Gansu Province 730070, China;
2. Key Laboratory of Superior Forage Germplasm in the Qinghai-Tibetan Plateau, Qinghai Academy of Animal Science and Veterinary Medicine, Qinghai University, Xining, Qinghai Province 810016, China

Received:2020-06-30 Revised:2020-08-04 Online:2020-12-15 Published:2020-12-02

摘要/Abstract

摘要： 为探究青海野生草地早熟禾（Poa pratensis）响应干旱胁迫的分子机制，本研究以青海野生草地早熟禾抗旱材料‘10-202’和敏感材料‘09-61’为研究对象，采用高通量Illumina Hiseq测序技术对15% PEG-6000模拟的干旱胁迫和正常处理下的2份材料的叶片进行差异基因表达分析。结果表明：‘10-202’处理间存在3 166个差异表达基因（Different expression genes，DEGs），上调和下调表达基因数目分别为1 979和1 187个；‘09-61’处理间共有314个DEGs，上调表达的有64个，下调表达的有250个。京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes，KEGG）富集分析发现，2份材料的差异基因均显著富集在淀粉和蔗糖代谢通路。运用MapMan软件对‘10-202’干旱胁迫下与转录调控相关的差异基因进行了功能注释，发现bHLH，AP2/EREPB及C2H2锌指家族转录因子在响应干旱胁迫中发挥着重要作用。本研究为后续挖掘草地早熟禾耐旱相关候选基因的克隆和功能验证奠定了理论基础。

关键词: 干旱胁迫, 青海野生草地早熟禾, 转录组, 差异表达基因

Abstract: In order to explore the molecular response mechanism of Qinghai wild Poa pratensis to drought stress,drought resistant material ‘10-202’ and sensitive material ‘09-61’ of Qinghai wild Kentucky bluegrass were selected as the research objects. High throughput Illumina Hiseq sequencing technology was used to analyze the differential gene expression of two materials under the drought stress simulated by 15% PEG-6000 and under the normal treatment. The results showed that:There were 3 166 differentially expressed genes (DEGs) in ‘10-202’ under different treatments,of which,the number of up-regulated and down-regulated expression genes were 1 979 and 1 187 respectively;there were 314 DEGs in different treatments of ‘09-61’,of which 64 were up-regulated and 250 were down-regulated. KEGG enrichment analysis showed that the difference genes of two wild Kentucky bluegrass materials were significantly enriched in starch and sucrose metabolism pathway. MapMan software was used to annotate the function of different genes related to transcription regulation under drought stress in ‘10-202’. It was found that bHLH,AP2/EREPB and C2H2 zinc finger family transcription factors played an important role in response to drought stress. These results laid a theoretical foundation for the cloning and functional verification of candidate genes related to drought tolerance of P. pratensis.

Key words: Drought stress, Qinghai wild Poa pratensis, Transcriptome, Differentially expressed genes

中图分类号:

S543

张然, 马祥, 朱瑞婷, 牛奎举, 赵春旭, 马晖玲. 青海野生草地早熟禾响应干旱胁迫的代谢通路及转录调控分析[J]. 草地学报, 2020, 28(6): 1508-1518.

ZHANG Ran, MA Xiang, ZHU Rui-ting, NIU Kui-ju, ZHAO Chun-xu, MA Hui-ling. Metabolic Pathway and Transcriptional Regulation of Qinghai Wild Poa pratensis in Response to Drought Stress[J]. Acta Agrestia Sinica, 2020, 28(6): 1508-1518.

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青海野生草地早熟禾响应干旱胁迫的代谢通路及转录调控分析

Metabolic Pathway and Transcriptional Regulation of Qinghai Wild Poa pratensis in Response to Drought Stress

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