内蒙古不同类型草地土壤氨氧化细菌和古菌的比较研究

doi:10.11733/j.issn.1007-0435.2021.12.004

草地学报 ›› 2021, Vol. 29 ›› Issue (12): 2664-2669.DOI: 10.11733/j.issn.1007-0435.2021.12.004

内蒙古不同类型草地土壤氨氧化细菌和古菌的比较研究

杨建强¹, 曹梦琪², 王袼³, 胡姝娅², 王常慧^2,3

1. 山西农业大学生命科学学院, 山西太谷 030801;
2. 中国科学院植物研究所植被与环境变化国家重点实验室, 北京 100093;
3. 山西农业大学草业学院, 山西太谷 030801

收稿日期:2021-05-31 修回日期:2021-07-13 出版日期:2021-12-15 发布日期:2022-01-06
作者简介:杨建强(1973-)，男，山西偏关人，博士，讲师，主要从事土壤微生物研究，E-mail：robert_free@126.com;曹梦琪(1998-)，女，山东济南人，硕士研究生，E-mail：caomengqi@ibcas.ac.cn
基金资助:
国家自然科学基金（31770526）；国家重点研发计划子课题（2017YFA0604802）；山西省博士毕业生、博士后研究人员来晋工作奖励资金科研项目（SXBYKY2021052）；山西农业大学博士科研启动项目（2021BQ26）资助

The Characteristics of Ammonia-oxidizing Bacteria and Ammonia-oxidizing Archaea in Different Grassland Types of Inner Mongolia

YANG Jian-qiang¹, CAO Meng-qi², WANG Ge³, HU Shu-ya², WANG Chang-hui^2,3

1. College of Life Science, Shanxi Agriculture University, Taigu, Shanxi Province 030801, China;
2. State Key Laboratory of Vegetation and Environmental Change, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China;
3. College of Grassland Science, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China

Received:2021-05-31 Revised:2021-07-13 Online:2021-12-15 Published:2022-01-06
Contact: 王常慧,E-mail:wangch@ibcas.ac.cn

摘要/Abstract

摘要： 氨氧化细菌（Ammonia-oxidizing bacteria，AOB）和氨氧化古菌（Ammonia-oxidizing archaea，AOA）是参与土壤硝化过程的关键微生物。本文以内蒙古3种草地类型（荒漠草原、典型草原和草甸草原）表层土壤为研究对象，利用qPCR技术测定土壤AOA，AOB的丰度，研究其与不同类型草地生物和环境因子之间的关系。结果表明：不同草地类型间AOA的丰度存在显著差异，典型草原>荒漠草原>草甸草原；但是AOB的丰度在不同草地类型间差异不显著。同一类型草地土壤AOA的丰度是AOB丰度的31~147倍。AOA丰度与海拔高度、土壤总有机碳含量、植物地下生物量、细菌和真菌的丰度呈显著正相关关系，与年均温、土壤容重和土壤pH值呈显著负相关关系；AOB丰度与细菌和真菌丰度、土壤总有机碳、微生物生物量碳/氮呈显著正相关关系，与年均温、土壤pH值呈显著负相关关系。AOA的丰度在不同空间尺度存在较大差异，而AOB丰度的差异较小。本研究结果有助于解释不同草地类型生产力差异的原因，为全球气候变化背景下草地土壤氮循环模型的完善提供数据支持。

关键词: 氨氧化细菌, 氨氧化古菌, 典型草原, 荒漠草原, 草甸草原

Abstract: Soil ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) are the key microorganisms for soil nitrification. In this study,surface soil sampled from three types of grassland,including desert steppe,typical steppe,and meadow steppe in Inner Mongolia,we measured the abundance of AOA and AOB using fluorescence quantitative PCR (qPCR) and analyzed the relationship between grassland type and environmental factors. The results showed a significant difference in the abundance of AOA among the three types of grassland in Inner Mongolia,which is typical steppe>desert steppe>meadow steppe. But no significant differences were found in the abundance of AOB among the three types of grassland. The abundance of AOA in the same type of grassland is 31~147 times that of AOB. The abundance of AOA was positively correlated with elevation,total organic carbon(TOC),underground biomass,the abundance of bacteria and fungi. The mean annual temperature(MAT) and bulk density were negatively correlated with soil pH. The abundance of AOB was positively correlated with bacteria fungi,TOC,MBC,MBN. MAT was negatively correlated with pH. The difference of the abundance of AOA is greater than that of AOB in different spatial scales. The results are helpful to explicate the reason of productivity difference in different types of grassland and could provide data supports for the improvement of grassland soil nitrogen cycle models.

Key words: AOB, AOA, Typical grassland, Meadow grassland, Desert grassland

中图分类号:

S154.3

杨建强, 曹梦琪, 王袼, 胡姝娅, 王常慧. 内蒙古不同类型草地土壤氨氧化细菌和古菌的比较研究[J]. 草地学报, 2021, 29(12): 2664-2669.

YANG Jian-qiang, CAO Meng-qi, WANG Ge, HU Shu-ya, WANG Chang-hui. The Characteristics of Ammonia-oxidizing Bacteria and Ammonia-oxidizing Archaea in Different Grassland Types of Inner Mongolia[J]. Acta Agrestia Sinica, 2021, 29(12): 2664-2669.

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参考文献

[1] DELWICHE C C. The nitrogen cycle[J]. Scientific American,1970,223(2):137-146
[2] KOWALCHUK G A,SETPHEN J R. Ammonia-oxidizing bacteria:a model for molecular microbial ecology[J]. Annual Review Microbiology,2001(55):485-529
[3] PROSSER J I,EMBLEY T M. Cultivation-based and molecular approaches to characterisation of terrestrial and aquatic nitrifiers[J]. Antonie Van Leeuwenhoek,2002,81(1-4):165-179
[4] PROSSER J I. Autotrophic nitrification in bacteria[J]. Advances Microbial Physiology,1990(30):125-181
[5] MARTIN K,ANNE B,CHRISTOPHER W,et al. Isolation of an autotrophic ammonia-oxidizing marine archaeon[J]. Nature,2005,437(7058):543-546
[6] 刘建国,刘卫国.微生物介导的氮循环过程研究进展[J].草地学报,2018,26(2):277-283
[7] HYMAN M R,ARP D J. 14C₂H₂ and 14CO₂-labeling studies of the de Novo Synthesis of Polypeptides by Nitrosomonus europaea during Recovery from Acetylene and Light Inactivation of Ammonia Monooxygenase[J]. The Journal of biological chemistry,1992,267(3):1534-1545
[8] PURKHOLD U,POMMERENING-RÖSER A,JURETSCHKO S,et al. Phylogeny of all recognized species of ammonia oxidizers based on comparative 16S rRNA and amoA sequence analysis:implications for molecular diversity surveys[J]. Applied and environmental microbiology,2000,66(12):5368-5382
[9] LI M,CAO H L,HONG Y G,et al. Spatial distribution and abundances of ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) in mangrove sediments[J]. Applied Microbiology Biotechnology,2011,89(4):1243-1254
[10] LEININGER S,URICH T,SCHLOTER M,et al. Archaea predominate among ammonia-oxidizing prokaryotes in soils[J]. Nature,2006,442(7104):806-809
[11] NICOL G W,LEININGER S,SCHLEPER C,et al. The influence of soil pH on the diversity,abundance and transcriptional activity of ammonia oxidizing archaea and bacteria[J]. Environental Microbiology,2008,10(11):2966-2978
[12] TOURNA M,FREITAG T E,NICOL,G W,et al. Growth,activity and temperature responses of ammonia-oxidizing archaea and bacteria in soil microcosms[J]. Environmental Microbiology,2008,10(5):1357-1364
[13] SHEN J P,ZHANG L M,ZHU Y G,et al. Abundance and composition of ammonia-oxidizing bacteria and ammonia-oxidizing archaea communities of an alkaline sandy loam[J]. Environmental Microbiology,2008,10(6):1601-1611
[14] HE J Z,SHEN J P,ZHANG L M,et al. Quantitative analyses of the abundance and composition of ammonia-oxidizing bacteria and ammonia-oxidizing archaea of a Chinese upland red soil under long-term fertilization practices[J]. Environmental Microbiology,2007,9(9):2364-2374
[15] XIANG X J,HE D,HE J S,et al. Ammonia-oxidizing bacteria rather than archaea respond to short-term urea amendment in an alpine grassland[J]. Soil Biology&Biochemistry,2017(107):218-225
[16] ADAIR K L,SCHWARTZ E. Evidence that Ammonia-Oxidizing Archaea are More Abundant than Ammonia-Oxidizing Bacteria in Semiarid Soils of Northern Arizona,USA[J]. Microbial Ecology,2008,56(3):420-426
[17] 洪义国,何翔,吴佳鹏,等.海洋氨氧化古菌及其驱动的碳氮生物地球化学循环过程研究进展[J].中国科学院大学学报,2020,37(4):433-441
[18] ZHANG L M,WANG M,PROSSER J I,et al. Altitude ammonia-oxidizing bacteria and archaea in soils of Mount Everest[J]. FEMS Microbiology Ecology,2009,70(2):208-217
[19] 朱蕊,陈清,马成仓,等.不同利用方式对内蒙古羊草草原氨氧化微生物丰度的影响[J].草地学报,2019,27(2):437-442
[20] DI H,CAMERON K,SHEN J,et al. Nitrification driven by bacteria and not archaea in nitrogen-rich grassland soils[J]. Nature Geoscience,2009,2(9):621-624
[21] 苏瑜,王为东.我国北方四类土壤中氨氧化古菌和氨氧化细菌的活性及对氨氧化的贡献[J].环境科学学报,2017,37(7):3519-3527
[22] LIU J,YU Z,YAO Q,et al. Ammonia-Oxidizing Archaea Show More Distinct Biogeographic Distribution Patterns than Ammonia-Oxidizing Bacteria across the Black Soil Zone of Northeast China[J]. Frontiers in microbiology,2018(9):171
[23] LIU J,YU Z,YAO Q,et al. Biogeographic Distribution Patterns of the Archaeal Communities Across the Black Soil Zone of Northeast China[J]. Frontiers in microbiology,2019(10):23
[24] 王晓胡,寇涌苹,赵文强,等.贡嘎山森林土壤氨氧化微生物数量在海拔梯度的空间分异特征[J].应用与环境生物学报,2019,25(2):275-280
[25] CHEN J,NIE Y,LIU W,et al. Ammonia-Oxidizing Archaea Are More Resistant Than Denitrifiers to Seasonal Precipitation Changes in an Acidic Subtropical Forest Soil[J]. Front in Microbiol,2017(8):1384
[26] TAO R,LI J,HU B W,et al. Ammonia-oxidizing bacteria are sensitive and not resilient to organic amendment and nitrapyrin disturbances,but ammonia-oxidizing archaea are resistan[J]. Geoderma,2021(384):114814
[27] ZHANG X,LIU W,SCHLOTER M,et al. Response of the abundance of key soil microbial nitrogen-cycling genes to multi-factorial global changes[J]. Plos One,2013,8(10):e76500

内蒙古不同类型草地土壤氨氧化细菌和古菌的比较研究

The Characteristics of Ammonia-oxidizing Bacteria and Ammonia-oxidizing Archaea in Different Grassland Types of Inner Mongolia

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