根瘤菌对紫花苜蓿生物修复镉污染功能的影响

doi:10.11733/j.issn.1007-0435.2021.12.016

草地学报 ›› 2021, Vol. 29 ›› Issue (12): 2763-2768.DOI: 10.11733/j.issn.1007-0435.2021.12.016

根瘤菌对紫花苜蓿生物修复镉污染功能的影响

孔海明, 李开源, 高双红, 孙娈姿

西北农林科技大学草业与草原学院, 陕西杨凌 712100

收稿日期:2021-05-06 修回日期:2021-07-10 出版日期:2021-12-15 发布日期:2022-01-06
作者简介:孔海明(2000-)，女，汉族，陕西西安人，在读本科生，主要从事牧草逆境生物学研究，E-mail：konghaimingm@163.com
基金资助:
陕西省重点研发项目（2020ZDLNY02-05）资助

Effects of Rhizobium on the Cadmium Bioremediation Function of Alfalfa

KONG Hai-ming, LI Kai-yuan, GAO Shuang-hong, SUN Luan-zi

College of Grassland Agriculture, Northwest A&F University, Yangling, Shaanxi Province 712100, China

Received:2021-05-06 Revised:2021-07-10 Online:2021-12-15 Published:2022-01-06
Contact: 孙娈姿,E-mail:sunluanzi@163.com

摘要/Abstract

摘要： 为研究根瘤菌对紫花苜蓿修复镉污染土壤的作用，本试验在低氮素（1 mmol·L^-1）和高氮素（4 mmol·L^-1）水平下对接种和不接种根瘤菌的紫花苜蓿（Medicago sativa L.）进行镉（CdCl₂）胁迫处理，测定处理后的株高、生物量、地上部和地下部镉含量、氮含量、叶片叶绿素和丙二醛含量。结果表明：在Cd胁迫下，提高氮素水平可显著增加植物株高和生物量、叶片叶绿素含量和Cd含量（P<0.05）；接种根瘤菌可提高紫花苜蓿氮素含量，增加植物的株高、生物量、叶片叶绿素含量和Cd含量，降低丙二醛含量（P<0.05）。综上所述，根瘤通过增加氮素含量缓解重金属Cd的毒害，并促进紫花苜蓿对Cd的吸收，表明根瘤菌在Cd污染土壤的修复中具有较大潜力和广阔的应用前景。

关键词: 紫花苜蓿, 镉胁迫, 根瘤固氮, 生物修复

Abstract: In order to study the effects of rhizobium on the remediation of cadmium contaminated soil by alfalfa (Medicago sativa L.),alfalfa was inoculated with or without rhizobia under low nitrogen (1 mmol·L^-1) and high nitrogen (4 mmol·L^-1) levels,and the plant height,biomass,cadmium content and nitrogen content in aboveground and belowground parts,chlorophyll and malondialdehyde content in leaves were measured. The results showed that under Cd stresses increasing nitrogen level could significantly increase plant height and biomass,chlorophyll content and Cd content in leaves (P<0.05). Inoculating rhizobia could increase nitrogen content,plant height,biomass,chlorophyll content and Cd content in leaves,and decrease MDA content (P<0.05). In conclusion,rhizobia could alleviate the toxicity of heavy metal Cd by increasing nitrogen content,and promote the absorption of Cd by alfalfa,indicating that rhizobium has great potential and broad application prospects in the remediation of Cd contaminated soil.

Key words: Alfalfa, Cadmium stress, Nodule nitrogen fixation, Bioremediation

中图分类号:

S541+.9

孔海明, 李开源, 高双红, 孙娈姿. 根瘤菌对紫花苜蓿生物修复镉污染功能的影响[J]. 草地学报, 2021, 29(12): 2763-2768.

KONG Hai-ming, LI Kai-yuan, GAO Shuang-hong, SUN Luan-zi. Effects of Rhizobium on the Cadmium Bioremediation Function of Alfalfa[J]. Acta Agrestia Sinica, 2021, 29(12): 2763-2768.

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参考文献

[1] 黄益宗,郝晓伟,雷鸣,等.重金属污染土壤修复技术及其修复实践[J].农业环境科学学报,2013,32(3):409-417
[2] 李婧,周艳文,陈森,等.我国土壤镉污染现状、危害及其治理方法综述[J].安徽农学通报,2015,21(24):104-107
[3] SINGH A,PRASAD S M. Remediation of heavy metal contaminated ecosystem:an overview on technology advancement[J]. International Journal of Environmental Science and Technology,2015,12(1):353-366
[4] 董姬妃,张帆,胡雨寒,等.镉胁迫下增施氮对白三叶草生长的影响和镉毒害的缓解效应研究[J].草业学报,2017,26(9):83-91
[5] 毕景文,刘秒,刘秀成,等.氮素与植物镉胁迫响应的研究进展[J].杭州师范大学学报(自然科学版),2020,19(1):1-9
[6] 李继光,金兰淑,陈禹桥,等.氮对低镉处理下东南景天根系形态及镉积累的影响[J].中国农学通报,2007,23(3):260-265
[7] LI J Y,FU Y L,PIKE S M,et al. The Arabidopsis nitrate transporter NRT1.8 functions in nitrate removal from the xylem sap and mediates cadmium tolerance[J]. Plant Cell,2010,22(5):1633-1646
[8] 蔡金宏,李治霖,王向涛,等.接种根瘤菌对于紫花苜蓿抗逆性的研究概况[J].黑龙江畜牧兽医,2020(9):47-49
[9] 刁亚南,赵腊梅,金海如.镉胁迫下豆科植物接种AM真菌和根瘤菌的生长及生理指标变化[J].贵州农业科学,2014,42(12):74-78
[10] 刘晓青,曹卫红,周卫红,等.农田土壤重金属污染的生物修复技术研究现状、问题及展望[J].天津农业科学,2018,24(2):80-85
[11] YOUNIS M. Responses of Lablab Purpureus-rhizobium symbiosis to heavy metals in pot and field experiments[J]. World Journal of Agricultural Sciences,2007,3(1):111-122
[12] CHEN W M,WU C H,JAMES E K,et al. Metal biosorption capability of Cupriavidus taiwanensis and its effects on heavy metal removal by nodulated Mimosa pudica[J]. Journal of Hazardous Materials,2008,151(2-3):364-371
[13] SHERIFI E,BYTYQI A,LLUGA-RIZANI K. The concentration of Pb and Cd to Medicago sativa L. along Lipjan-Prizren highway and their influence on biomass[J]. Journal of Engineering and Applied Sciences,2009,4(1):60-63
[14] 张杨杨,李希铭,高鹏,等.不同浓度镉胁迫下6种草本植物的耐性及富集特征的比较[J].草地学报,2021,29(6):1265-1276
[15] 杨子文,沈禹颖,谢田玲,等.外源供氮水平对大豆生物固氮效率的影响[J].西北植物学报,2009,29(3):574-579
[16] SHEN M,LIU L,LI D W,et al. The effect of endophytic Peyronellaea from heavy metal-contaminated and uncontaminated sites on maize growth,heavy metal absorption and accumulation[J].Fungal Ecology,2013,6(6):539-545
[17] 李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:260-261
[18] SARTORY D P,GROBBELAAR J U. Extraction of chlorophyll a from freshwater phytoplankton for spectrophotometric analysis[J]. Hydrobiologia,1984,114(3):177-187
[19] 徐苏凌,方勇,邢承华.镉胁迫对紫花苜蓿生长及结瘤的影响[J].江西农业大学学报,2008,30(6):1049-1052
[20] KHAN N A,SINGH S,NAZAR R. Activities of antioxidative enzymes,sulphur assimilation,photosynthetic activity and growth of wheat (Triticum aestivum) cultivars differing in yield potential under cadmium stress[J]. Journal of Agronomy and Crop Science,2010,193(6):435-444
[21] LUX A,MARTINKA M,VACULíK M,et al. Root responses to cadmium in the rhizosphere:a review[J]. Journal of Experimental Botany,2011,62(1):21-37
[22] 韩可,孙彦,张昆,等.接种不同根瘤菌对紫花苜蓿生产力的影响[J].草地学报,2018,26(3):639-644
[23] 王辉,许超,罗尊长,等.氮肥用量对油菜吸收积累镉的影响[J].水土保持学报,2017,31(6):302-305
[24] 邓小红,姬拉拉,王健健.施氮对镉胁迫下山杨幼苗叶片氮磷钾吸收及镉积累量的影响[J].西北植物学报,2020,40(11):1932-1939
[25] 曹玲,王庆成,崔东海.土壤镉污染对四种阔叶树苗木叶绿素荧光特性和生长的影响[J].应用生态学报,2006,17(5):769-772
[26] 热比姑丽·热合曼,郭海玉,等.地下滴灌条件下不同接种处理对苜蓿根瘤菌的定殖、结瘤和固氮的影响[J].草地学报,2018,26(2):479-487
[27] 王鹏辉,姜昕,马鸣超,等.一株耐干燥大豆根瘤菌菌株的筛选与固氮效果评价[J].大豆科学,2020,39(1):90-96
[28] BRAHIM S,JOKE D,ANN C,et al. Leaf proteome responses of Arabidopsis thaliana exposed to mild cadmium stress[J]. Journal of plant physiology,2010,167(4):247-254
[29] CUI Y,WANG Q. Physiological responses of maize to elemental sulphur and cadmium stress[J]. Plant,Soil and Environment,2006,64:523-529
[30] HASSAN M J,WANG F,ALI S,et al. Toxic effect of cadmium on rice as affected by nitrogen fertilizer form[J]. Plant and Soil,2005,277(1-2):359-365

根瘤菌对紫花苜蓿生物修复镉污染功能的影响

Effects of Rhizobium on the Cadmium Bioremediation Function of Alfalfa

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