两种诱变处理下紫花苜蓿全基因组DNA甲基化图谱分析

doi:10.11733/j.issn.1007-0435.2022.01.006

草地学报 ›› 2022, Vol. 30 ›› Issue (1): 46-54.DOI: 10.11733/j.issn.1007-0435.2022.01.006

• 研究论文 • 上一篇

两种诱变处理下紫花苜蓿全基因组DNA甲基化图谱分析

申晓慧

宜春学院，生命科学与资源环境学院，江西宜春 336000

收稿日期:2021-07-08 修回日期:2021-09-06 发布日期:2022-01-27
作者简介:申晓慧(1980-)，女，汉族，吉林扶余人，博士后，助理研究员，主要从事作物逆境生理研究，E-mail: xiaohuishen@126.com
基金资助:
博士科研启动项目(210-3360119010)资助

Analysis of DNA Methylation Map of Alfalfa Whole Genome under Two Kinds of Mutagenesis Treatments

SHEN Xiao-hui

College of Life Sciences, Resources and Environment Sciences, Jiangxi Province, Yichun University, Yichun 336000, China

Received:2021-07-08 Revised:2021-09-06 Published:2022-01-27

摘要/Abstract

摘要： 为了探究不同诱变处理对紫花苜蓿表观遗传学效应的影响，试验采用甲基磺酸乙酯(EMS)和⁶⁰CO-γ两种诱变方法对紫花苜蓿种子进行处理，采用重亚硫酸盐测序(BS-seq)技术，对紫花苜蓿诱变前后全基因组DNA甲基化图谱进行分析。结果表明，紫花苜蓿体内的DNA甲基化主要有mCG，mCHH和mCHG 3种类型；C位点发生甲基化的比率在EMS诱变处理下表现为上升，在⁶⁰CO-γ诱变处理下表现为下降。此外，DNA甲基化主要发生在CG序列；对不同诱变处理组的差异甲基化区域(DMR)鉴定发现有105个基因在两组比较中均存在DMR，KEGG通路富集分析表明，在DMR相关基因中显著富集的通路主要包括氮代谢通路(Nitrogen metabolism)、淀粉和蔗糖代谢途径(Starch and sucrose metabolism)、脯氨酸代谢(Proline metabolism)等生物学过程。因此，诱变改变了苜蓿DNA甲基化水平，为诱变突变体的筛选提供了丰富材料。

关键词: 紫花苜蓿, DNA甲基化, 重亚硫酸盐测序, 表观遗传

Abstract: To investigate the epigenetic effects of different mutagenic treatments on alfalfa, ethyl methanesulfonate (EMS) and ⁶⁰Co-γ were used to treat alfalfa seeds. The whole-genome DNA methylation map of alfalfa before and after mutation was analyzed by bisulfite-sequencing (BS-seq). The results showed that there were three types of DNA methylation in alfalfa: mCG、mCHH and mCHG. The methylation rate of C site increased under EMS mutagenesis and decreased at ⁶⁰Co-γ mutagenesis. In addition, DNA methylation mainly occurred in CG sequence. The identification of differential methylation region (DMR) of different mutation treatment groups found that 105 genes had DMR in two groups. KEGG pathway enrichment analysis showed that the pathways significantly enriched in DMR related genes mainly included nitrogen metabolism pathway, starch, and sucrose metabolism pathway, and proline metabolism processes. Therefore, mutagenesis changed the DNA methylation level of alfalfa and provided abundant materials for the screening of mutagenic mutants.

Key words: Alfalfa, DNA methylation, Bisulfite-sequence, Epigenetics

中图分类号:

S816.4

申晓慧. 两种诱变处理下紫花苜蓿全基因组DNA甲基化图谱分析[J]. 草地学报, 2022, 30(1): 46-54.

SHEN Xiao-hui. Analysis of DNA Methylation Map of Alfalfa Whole Genome under Two Kinds of Mutagenesis Treatments[J]. Acta Agrestia Sinica, 2022, 30(1): 46-54.

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两种诱变处理下紫花苜蓿全基因组DNA甲基化图谱分析

Analysis of DNA Methylation Map of Alfalfa Whole Genome under Two Kinds of Mutagenesis Treatments

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