祁连山云杉林土壤溶磷细菌的分离及对白三叶的促生效应

doi:10.11733/j.issn.1007-0435.2022.04.013

草地学报 ›› 2022, Vol. 30 ›› Issue (4): 879-888.DOI: 10.11733/j.issn.1007-0435.2022.04.013

• 研究论文 • 上一篇

祁连山云杉林土壤溶磷细菌的分离及对白三叶的促生效应

李慧萍, 甘雅楠, 韩庆庆, 姚丹, 陈佳, 张明旭, 何傲蕾, 张金林, 赵祺

兰州大学农业农村部草牧业创新重点实验室, 兰州大学草地微生物研究中心, 兰州大学草地农业生态系统国家重点实验室, 兰州大学草地农业科技学院, 甘肃兰州 730020

收稿日期:2021-09-29 修回日期:2021-11-05 发布日期:2022-04-25
通讯作者: 张金林,E-mail:jlzhang@lzu.edu.cn;赵祺,E-mail:qzhao@lzu.edu.cn
作者简介:李慧萍(1994-),女,汉族,甘肃古浪人,博士研究生,主要从事极端环境微生物多样性和根际促生细菌的研究,E-mail:lihp19@lzu.edu.cn
基金资助:
国家自然科学基金项目(31801944);中央高校基本科研业务费专项资金(lzujbky-2021-ct16);国家重点研发计划(2019YFC0507703)资助

Isolation of Phosphate Solubilizing Bacteria from Soil of Spruce Forest in the Qilian Mountains and Their Growth Promotion Effects in White Clover

LI Hui-ping, GAN Ya-nan, HAN Qing-qing, YAO Dan, CHEN Jia, ZHANG Ming-xu, HE Ao-lei, ZHANG Jin-lin, ZHAO Qi

Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs, Center for Grassland Microbiome, State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, Gansu Province 730020, China

Received:2021-09-29 Revised:2021-11-05 Published:2022-04-25

摘要/Abstract

摘要： 为获得具有促进牧草白三叶(Trifolium repens L.)生长能力的优良溶磷细菌(Phosphate solubilizing bacteria,PSB)资源,本研究以祁连山青海云杉林(Picea crassifolia)根际土壤细菌为研究对象,采用平板定性与钼锑抗比色定量分析、系统进化分析和植物盆栽接种试验,探究了上述根际土壤所分离菌株的溶磷能力、分类地位和对白三叶生长的影响。结果表明:筛选得到的12株PSB菌株在难溶性无机磷(National botanical research institute phosphorus,NBRIP)平板上的溶磷圈直径/菌落直径的比值范围为1.44~2.40。12株PSB菌株均归属于假单胞菌属(Pseudomonas sp.)。在上述基础上,选取的5株菌株在NBRIP液体培养基培养96 h后,发酵液的可溶性磷含量范围为387.41~479.87 μg·mL^-1,其中菌株LT-4发酵液的可溶性磷含量最高。植物接种试验结果表明,与空白对照相比,接种菌株LT-4能显著提高白三叶的生物量,植株地上部和根干重分别显著增加87.01%和74.83%。本研究为进一步探究溶磷细菌的促生机理以及开发应用于栽培牧草的微生物菌剂或菌肥奠定了一定的前期工作基础。

关键词: 溶磷细菌, 假单胞菌属, 白三叶, 促生效应

Abstract: In order to obtain excellent phosphate solubilizing bacteria (PSB) strains that could promote the growth of white clover (Trifolium repens L.),soil collected from spruce forests (Picea crassifolia) in the Qilian Mountains were served as our research object in this study.We used phosphate-dissolving qualitative and quantitative methods,phylogenetic analysis and plant inoculation experiments to investigate the phosphate solubilizing ability,taxonomic status of PSB strains and evaluate their inoculation effects on the growth of white clover.The results showed that the ratio of the diameters of the halo to the colony on NBRIP plates of 12 PSB strains were ranging from 1.44 to 2.40.These strains were further identified and classified as Pseudomonas sp..On the basis of above data,the soluble phosphorus content of 5 selected PSB strains was ranging from 387.41 μ g·mL^-1 to 479.87 μ g·mL^-1 in the NBRIP liquid medium after 96 hours of incubation.The content of soluble phosphorus in the fermentation broth of strain LT-4 was the highest in all strains.In addition,we observed that the application of strain LT-4 significantly improved the growth of white clover compared to DH5α and water treatment,respectively,especially,shoot and root dry weight significantly increased by 87.01% and 74.83%.These results could lay a foundation for further exploring the regulatory mechanism and high-efficiency biofertilizer for forage cultivation.

Key words: Phosphate-solubilizing bacteria, Pseudomonas, White clover, Growth-promotion

中图分类号:

Q93-Q939.3

李慧萍, 甘雅楠, 韩庆庆, 姚丹, 陈佳, 张明旭, 何傲蕾, 张金林, 赵祺. 祁连山云杉林土壤溶磷细菌的分离及对白三叶的促生效应[J]. 草地学报, 2022, 30(4): 879-888.

LI Hui-ping, GAN Ya-nan, HAN Qing-qing, YAO Dan, CHEN Jia, ZHANG Ming-xu, HE Ao-lei, ZHANG Jin-lin, ZHAO Qi. Isolation of Phosphate Solubilizing Bacteria from Soil of Spruce Forest in the Qilian Mountains and Their Growth Promotion Effects in White Clover[J]. Acta Agrestia Sinica, 2022, 30(4): 879-888.

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参考文献

[1] 钟传青.解磷微生物溶解磷矿粉和土壤难溶磷的特性及其溶磷方式研究[D].南京:南京农业大学,2004:25-26
[2] 易艳梅,黄为一.产多糖溶磷细菌对难溶性Ca-P的活化特性[J].南京农业大学学报,2008(2):49-54
[3] 鲁如坤.我国的磷矿资源和磷肥生产消费Ⅱ.磷肥消费和需求[J].土壤,2004(2):113-116
[4] LYNCH J P. Root phenes for enhanced soil exploration and phosphorus acquisition:tools for future crops[J]. Plant Physiology,2011,156(3):1041-1049
[5] 盛荣,肖和艾,谭周进,等.土壤解磷微生物及其磷素有效性转化机理研究进展[J].土壤通报,2010,41(6):1505-1510
[6] THAKUR M P,VANDER W H,COBBEN M M P,et al. Microbial invasions in terrestrial ecosystems[J]. Nature Reviews Microbiology,2019,17(10):621-631
[7] MOHAMMADI K. Phosphorus solubilizing bacteria:occurrence,mechanisms and their role in crop production[J]. Resources and Environment,2012,2(1):80-85
[8] ALORI E T,GLICK B R,BABALOLA O O. Microbial phosphorus solubilization and its potential for use in sustainable agriculture[J]. Frontiers in Microbiology,2017(8):971
[9] WANI P A,KHAN M S,ZAIDI A. Chromium reduction,plant growth-promoting potentials,and metal solubilization by Bacillus sp. isolated from alluvial soil[J]. Current Microbiology,2007,54(3):237-243
[10] ZAIDI A,KHAN M S,AHEMAD M,et al. Plant growth promotion by phosphate solubilizing bacteria[J]. Acta Microbiologica Et Immunologica Hungarica,2009,56(3):263-284
[11] ILLMER P,SCHINNER F. Solubilization of inorganic phosphates by microorganisms isolated from forest soils[J]. Soil Biology and Biochemistry,1992,24(4):389-395
[12] 吕俊,于存.一株高效溶磷伯克霍尔德菌的筛选鉴定及对马尾松幼苗的促生作用[J].应用生态学报,2020,31(9):2923-2934
[13] 朱平,陈仁升,宋耀选,等.祁连山中部4种典型植被类型土壤细菌群落结构差异[J].生态学报,2017,37(10):3505-3514
[14] 刘小平,张富.青海云杉林生物量及地上径向密度随海拔的变化特征[J].林业科技通讯,2021(10):32-35
[15] 赵维俊.祁连山青海云杉林群落结构特性及养分特征研究[D].兰州:甘肃农业大学,2016:22
[16] LAN X J,ZHOU H,YAO T,et al. Community structure and function of cultivable endophytic bacteria isolated from four moss species in Qilian Mountain[J]. Symbiosis,2020,80(2):257-267
[17] 马剑,刘贤德,李广,等.祁连山北麓中段青海云杉林土壤水热时空变化特征[J].干旱区地理,2020,43(4):1033-1040
[18] 张宇龙,卢小良,杨成德.东祁连山高寒草地土壤无机磷溶解菌分离及溶磷能力初探[J].草地学报,2011,19(4):560-564
[19] 吴永洁,王元素,李莉.国内白三叶研究进展[J].种子,2015,34(7):46-51
[20] 赵祺.冻土细菌新种和冰缘植物内生细菌新种的特征和多相分类学研究[D].兰州:兰州大学,2010:39-41
[21] 李惠茹.旱生植物梭梭根际可培养细菌多样性分析[D].兰州:兰州大学,2016:17-18
[22] MARRA L M, OLIVEIRA S M,SOARES C R F S,et al. Solubilisation of inorganic phosphates by inoculant strains from tropical legumes[J]. Scientia Agricola,2011,68(5):603-609
[23] LIU Z,LI Y C,ZHANG S,et al. Characterization of phosphate-solubilizing bacteria isolated from calcareous soils[J]. Applied Soil Ecology,2015(96):217-224
[24] LIU F P,LIU H Q,ZHOU H L,et al. Isolation and characterization of phosphate-solubilizing bacteria from betel nut (Areca catechu) and their effects on plant growth and phosphorus mobilization in tropical soils[J]. Biology and Fertility of Soils,2014,50(6):927-937
[25] 韩玉竹,赵建军,曾兵,等.多花黑麦草根际解磷菌的分离及解磷能力测定[J].草地学报,2011,19(5):766-770
[26] HE A,NIU S,ZHAO Q,et al. Induced salt tolerance of perennial ryegrass by a novel bacterium strain from the rhizosphere of a desert shrub Haloxylon ammodendron[J]. International Journal of Molecular Sciences,2018,19(2):469
[27] ZHU J,LI M,WHELAN M. Phosphorus activators contribute to legacy phosphorus availability in agricultural soils:A review. Science of the Total Environment[J]. 2018(612):522-537
[28] 赵天雪,臧飞,李娜,等.祁连山青海云杉林内穿透雨中微量元素变化特征[J/OL].西北林学院学报,2021(5):36-41
[29] 李玉娥,姚拓,荣良燕.溶磷菌溶磷和分泌IAA特性及对苜蓿生长的影响[J].草地学报,2010,18(1):84-88
[30] 张英,芦光新,谢永丽,等.溶磷菌分泌有机酸与溶磷能力相关性研究[J].草地学报,2015,23(5):1033-1038
[31] 韩丽珍,周静,王欢.两株对花生促生的芽孢杆菌的鉴定及溶磷特性研究[J].基因组学与应用生物学,2019,38(9):4066-4076
[32] PANDE A,PANDEY P,MEHRA S,et al. Phenotypic and genotypic characterization of phosphate solubilizing bacteria and their efficiency on the growth of maize[J]. Journal of Genetic Engineering and Biotechnology,2017,15(2):379-391
[33] MAHANTA D,RAI R K,DHAR S,et al. Modification of root properties with phosphate solubilizing bacteria and arbuscular mycorrhiza to reduce rock phosphate application in soybean-wheat cropping system[J]. Ecological Engineering,2018(111):31-43
[34] 汪焱,张英,苏贝贝,等.溶磷菌对3种牧草种子发芽和幼苗生长的影响[J].青海畜牧兽医杂志,2019,49(6):1-5
[35] TAURIAN T,ANZUAY M S,ANGELINI J G,et al. Phosphate-solubilizing peanut associated bacteria:screening for plant growth-promoting activities[J]. Plant and Soil,2010,329(s1-2):421-431

祁连山云杉林土壤溶磷细菌的分离及对白三叶的促生效应

Isolation of Phosphate Solubilizing Bacteria from Soil of Spruce Forest in the Qilian Mountains and Their Growth Promotion Effects in White Clover

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