关键词: 白三叶, 杂种鉴定, SSR分子标记, 遗传多样性, 表型分析

Abstract: Early and rapid identification of F1 hybrid progeny and acquisition of true hybrids are of great significance to the breeding and genetic research of Trifolium repens superior strains. Six phenotypic traits of 2 Trifolium repens parent materials and 55 F1 hybrids were mapped by clustering heat map,and 3 pairs of specific SSR primers were selected to identify the authenticity of F1 hybrids. The results showed that the 57 materials in the cluster heat map could be divided into 2 classes according to the phenotypic characters of their parents. A total of 281 distinct bands and 137 polymorphic bands were obtained by amplification with 19 pairs of selected primers. The polymorphic sites accounted for 48.41%. The PIC of each SSR site ranged from 18.49% to 43.58%,with an average value of 34.73%. A total of 53 true-hybrids were identified by three pairs of specific primers,and the true-hybrid ratio was 96.36%,which was basically consistent with the results of UPGMA cluster analysis. The 19 pairs of SSR molecular marker primers have high polymorphism,which can be directly used for genetic diversity analysis of Trifolium repens and hybrid identification and other related studies. The identified F1 generation true hybrids of Trifolium repens can lay a foundation for subsequent breeding work.

Key words: Trifolium repens, Identification of Hybrid, SSR molecular marker, Genetic diversity, Phenotypic analysis