藏北高寒草原典型植物根际土壤细菌群落结构多样性及根系特征分析

doi:10.11733/j.issn.1007-0435.2022.05.013

草地学报 ›› 2022, Vol. 30 ›› Issue (5): 1131-1140.DOI: 10.11733/j.issn.1007-0435.2022.05.013

藏北高寒草原典型植物根际土壤细菌群落结构多样性及根系特征分析

付莉娇^1,2, 李雪琴^1,2, 范继辉¹, 鲁旭阳¹, 鄢燕¹

1. 中国科学院、水利部成都山地灾害与环境研究所山地表生过程与生态调控重点实验室, 四川成都 610041;
2. 中国科学院大学, 北京 100049

收稿日期:2021-10-28 修回日期:2021-11-26 出版日期:2022-05-15 发布日期:2022-06-06
通讯作者: 鄢燕,E-mail:yy@imde.ac.cn
作者简介:付莉娇(1998-),女,汉族,四川简阳人,硕士研究生,主要从事高寒生态学研究,E-mail:fulijiao@imde.ac.cn
基金资助:
国家自然科学基金项目(41871049,41871072)资助

Analysis of Rhizosphere Soil Bacterial Community Structure Diversity and Root Characteristics of Typical Plants in Alpine Steppe of Northern Tibet

FU Li-jiao^1,2, LI Xue-qin^1,2, FAN Ji-hui¹, LU Xu-yang¹, YAN Yan¹

1. Key Laboratory of Mountain Surface Processes and Ecological Regulation, Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu, Sichuan Province 610041, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China

Received:2021-10-28 Revised:2021-11-26 Online:2022-05-15 Published:2022-06-06

摘要/Abstract

摘要： 根际土壤微生物作为根系生境中的重要组成部分，在物质能量循环方面发挥着不可替代的作用。本研究选择3种藏北高寒草原上具有代表性的植物，采用高通量测序方法揭示了不同植物根际细菌群落结构多样性差异，并利用WinRHIZO根系图像分析系统软件分析各植物的根系形态差异。结果表明：Acidobacteria (酸杆菌门)为所有样本中的优势菌门，占所有门类的25%左右；根际细菌群落在组间的差异均大于组内差异，但不显著；在测定的6个土壤环境因子中，速效磷是影响根际土壤微生物群落结构及其多样性的关键因子；与流水带组相比，对照组的根系平均直径减小，根尖数均显著增多(P<0.05)，表明流水带内外3种植物间的总根系形态特征存在差异。由此可知，流水带内外的植物地下部分主要通过调整根际土壤微生物群落结构和调节根系形态来适应生境的变化。

关键词: 流水, 根际微生物, 群落结构, 根系特征

Abstract: As an important part of root habitat,rhizosphere soil microorganisms play an irreplaceable role in material and energy cycle. In this study,three representative plants from alpine steppe in northern Tibet were selected to reveal the diversity of bacterial community structure in the rhizosphere of different plants by high-throughput sequencing,and the root morphological differences of each plant were analyzed by WinRHIZO root image analysis system. The results showed that Acidobacteria was the dominant phylum in all samples,accounting for about 25% of all phyla. The difference of rhizosphere soil bacterial community between groups was greater than that within groups,but it was not significant. Among the six soil environmental factors,available phosphorus is the key factor affecting the structure and diversity of microbial community in rhizosphere soil. Compared with the running water zone group,the average root diameter of the control group decreased and the number of root tips increased significantly(P<0.05),indicating that there were differences in the morphological characteristics of the total root system among the three plants inside and outside the running water zone. It can be seen that the underground part of plants inside and outside the running water zone can adapt to the changes of habitat mainly by adjusting the microbial community structure of rhizosphere soil and the morphology of root system.

Key words: Running water, Rhizosphere microorganism, Community structure, Root characteristics

中图分类号:

S154

付莉娇, 李雪琴, 范继辉, 鲁旭阳, 鄢燕. 藏北高寒草原典型植物根际土壤细菌群落结构多样性及根系特征分析[J]. 草地学报, 2022, 30(5): 1131-1140.

FU Li-jiao, LI Xue-qin, FAN Ji-hui, LU Xu-yang, YAN Yan. Analysis of Rhizosphere Soil Bacterial Community Structure Diversity and Root Characteristics of Typical Plants in Alpine Steppe of Northern Tibet[J]. Acta Agrestia Sinica, 2022, 30(5): 1131-1140.

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https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2022/V30/I5/1131

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藏北高寒草原典型植物根际土壤细菌群落结构多样性及根系特征分析

Analysis of Rhizosphere Soil Bacterial Community Structure Diversity and Root Characteristics of Typical Plants in Alpine Steppe of Northern Tibet

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