有机肥改变了退化人工草地微生物群落网络结构和潜在功能

doi:10.11733/j.issn.1007-0435.2023.09.013

草地学报 ›› 2023, Vol. 31 ›› Issue (9): 2693-2702.DOI: 10.11733/j.issn.1007-0435.2023.09.013

有机肥改变了退化人工草地微生物群落网络结构和潜在功能

周学丽^1,2, 史德军², 刘汉江², 丁建昭², 马军², 张海娟¹, 颜珲璘¹, 王英成¹, 金鑫¹, 祁鹤兴¹, 芦光新¹

1. 青海大学农牧学院, 青海西宁 810016;
2. 青海省草原改良试验站, 青海共和 813099

收稿日期:2023-02-21 修回日期:2023-03-27 出版日期:2023-09-15 发布日期:2023-10-07
通讯作者: 芦光新,E-mail:lugx74@qq.com
作者简介:周学丽(1979-),女,汉族,青海乐都人,博士研究生,主要从事牧草育种与栽培、草地生态与环境保护研究,E-mail:zhouxuelia@163.com
基金资助:
青海省科技厅重点研发与转化计划科技国际合作专项(2023-HZ-803):青海草地退化/恢复过程中土壤有机质转化的微生物学机制研究项目资助

Organic Fertilizer Application Changed the Structure and Potential Functions of Microbial Communities Networks in Degraded Artificial Grasslands

ZHOU Xue-li^1,2, SHI De-jun², LIU Han-jiang², DING Jian-zhao², MA Jun², ZHANG Hai-juan¹, YAN Hui-lin¹, WANG Ying-cheng¹, JIN Xin¹, QI He-xing¹, LU Guang-xin¹

1. College of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai Province 810016, China;
2. Qinghai Grassland Improvement Experimental Station, Gonghe, Qinghai Province 813099, China

Received:2023-02-21 Revised:2023-03-27 Online:2023-09-15 Published:2023-10-07

摘要/Abstract

摘要： 以环青海湖地区的退化人工草地为研究对象,探究施有机肥对土壤微生物群落中不同物种之间的相互作用以及物种功能特征的影响。结果表明:施有机肥显著改变了退化人工草地植被盖度及土壤理化性质(P<0.05)。同时,也改变了草地土壤微生物群落物种组成和结构,对细菌群落而言,施有机肥降低了绿弯菌门(Chloroflexi)、巴纽尔斯菌门(Balneolaeota)、螺旋体门(Spirochaetes)和Candidatus Saccharibacteria的相对丰度(P<0.05),增加了Gp7和不动杆菌属(Acinetobacter)的相对丰度(P<0.05),而对真菌群落相对丰度的改变不显著。单边网络结构在施用有机肥后表现出更为复杂和紧密的关系,且改变了人工草地微生物网络结构中的核心物种。本研究结果表明施用有机肥会对退化人工草地土壤微生物群落结构以及物种相互作用产生更大的影响、提供了建立恢复草地微生物动态变化的重要见解。

关键词: 退化人工草地, 有机肥, 微生物多样性, 群落结构, 单边网络

Abstract: The degraded artificial grassland in the Qinghai Lake area was used as a research object to investigate the effects of organic fertiliser application on the interactions between different species in the soil microbial community and the functional characteristics of the species. The results showed that the application of organic fertiliser significantly altered the forage cover and soil physicochemical properties of degraded artificial grassland (P<0.05). It also altered the species composition and structure of the soil microbial community of the grassland. For the bacterial community,the application of organic fertilizer reduced the relative abundance of Chloroflexi,Balneolaeota,Spirochaetes and Candidatus Saccharibacteria (P<0.05),and increased the relative abundance of Gp7 and Acinetobacter (P<0.05);while the change in the relative abundance of fungal communities was not significant. The unilateral network structure showed a more complex and tighter relationship after organic fertiliser application and altered the core species in the microbial grid structure of artificial grasslands.The results of this study suggest that the application of organic fertiliser has a greater impact on soil microbial community structure and species interactions in degraded artificial grasslands,and the results of this study provide important insights into establishing changes in microbial dynamics in the restored grasslands.

Key words: Degraded artificial grassland, Organic fertilizer, Microbial diversity, Community structure, Unilateral networks

中图分类号:

S283

周学丽, 史德军, 刘汉江, 丁建昭, 马军, 张海娟, 颜珲璘, 王英成, 金鑫, 祁鹤兴, 芦光新. 有机肥改变了退化人工草地微生物群落网络结构和潜在功能[J]. 草地学报, 2023, 31(9): 2693-2702.

ZHOU Xue-li, SHI De-jun, LIU Han-jiang, DING Jian-zhao, MA Jun, ZHANG Hai-juan, YAN Hui-lin, WANG Ying-cheng, JIN Xin, QI He-xing, LU Guang-xin. Organic Fertilizer Application Changed the Structure and Potential Functions of Microbial Communities Networks in Degraded Artificial Grasslands[J]. Acta Agrestia Sinica, 2023, 31(9): 2693-2702.

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https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2023/V31/I9/2693

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有机肥改变了退化人工草地微生物群落网络结构和潜在功能

Organic Fertilizer Application Changed the Structure and Potential Functions of Microbial Communities Networks in Degraded Artificial Grasslands

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