药用植物卷叶黄精根际解磷细菌的筛选鉴定及培养条件的优化

doi:10.11733/j.issn.1007-0435.2026.04.032

草地学报 ›› 2026, Vol. 34 ›› Issue (4): 1510-1523.DOI: 10.11733/j.issn.1007-0435.2026.04.032

• 技术研发 • 上一篇

药用植物卷叶黄精根际解磷细菌的筛选鉴定及培养条件的优化

杨维^1,2,3,4, 马振林^1,2,3,4, 闫刚^1,2,3,4, 王泽^1,2,3,4, 岳辰^1,2,3,4, 刘航^1,2,3,4, 王瑞红^1,2,3,4, 张新军^1,2,3,4

1. 西藏农牧大学高原生态研究所, 西藏林芝 860000;
2. 西藏高原森林生态教育部重点实验室, 西藏林芝 860000;
3. 西藏林芝高山森林生态系统国家野外科学观测研究站, 西藏林芝 860000;
4. 西藏自治区高寒植被生态安全重点实验室, 西藏林芝 860000

收稿日期:2025-06-11 修回日期:2025-08-25 发布日期:2026-04-15
通讯作者: 张新军，E-mail：zxjun_126abc@126.com
作者简介:杨维（1999-），女，土家族，贵州铜仁人，硕士研究生，主要从事微生物的研究，E-mail：3679030032@qq.com；
基金资助:
西藏自治区科技计划项目（XZ202301ZR0047G，XZ202301YD0028C）；国家自然科学基金（31960013）；西藏农牧学院研究生教育创新计划项目（YJS2021-22）；西藏农牧大学研究生教育创新计划项目（YJS-202443）；2024年度西藏高原森林生态教育部重点实验室研究生创新计划项目（XZAJYBSYS-202409，XZAJYBSYS-08）资助

Screening， Identification and Optimization of Culture Conditions of Phosphate-Solubilizing Bacteria in the Rhizosphere of Medicinal Plant Polygonatum cirrhifolium

YANG Wei^1,2,3,4, MA Zhen-lin^1,2,3,4, YAN Gang^1,2,3,4, WANG Ze^1,2,3,4, YUE Chen^1,2,3,4, LIU Hang^1,2,3,4, WANG Rui-hong^1,2,3,4, ZHANG Xin-jun^1,2,3,4

1. Institute of Xizang Plateau Ecology, Xizang Agriculture and Animal Husbandry University, Linzhi, Xizang 860000, China;
2. Key Laboratory of Forest Ecology of Xizang Plateau, Ministry of Education, Linzhi, Xizang 860000, China;
3. National Forest Ecosystem Observation&. Research Station of Linzhi XiZang, Linzhi, Xizang 860000, China;
4. Key Laboratory of Alpine Vegetation Ecological Security in Xizang, Linzhi, Xizang 860000, China

Received:2025-06-11 Revised:2025-08-25 Published:2026-04-15

摘要/Abstract

摘要： 解决土壤磷缺失现状，开发新型、安全、高效的微生物菌肥。本研究以西藏卷叶黄精（Polygonatum cirrhifolium）为试验材料，采用“划线法”筛选具有解磷能力的菌株，测定其解磷能力，并对其培养条件进行研究。结果显示，本研究共分离获得9株解磷细菌，筛选到5株溶磷指数>2.0的解磷菌株，解磷量为29.603~41.730 mg·Ｌ^-1；鉴定菌株JYHJ-1为Pseudomonas yamanorum，JYHJ-2为Pseudomonas koreensis，JYHJ-3为Serratia proteamaculans，JYHJ-4为Pseudomonas azotoformans，JYHJ-5为Microbacterium oxydans。菌株JYHJ-3的解磷能力最强，且可分泌7种有机酸和具产铁载体、解钾、固氮及产吲哚乙酸等能力。单因素试验中，碳源以乳糖、氮源以乙酸铵、初始pH值为5.5、接种量2%、NaCl浓度1%时菌株解磷效果最优。在单因素试验基础上，采用响应面试验对初始pH值、接种量、NaCl浓度进行优化，最终确定最佳培养条件为：乳糖为碳源、乙酸铵为氮源、初始pH值为5.5、接种量为3%、NaCl为1%。综上，卷叶黄精根际解磷细菌资源丰富，可为药用卷叶黄精可持续栽培及微生物肥料开发提供菌株资源与理论依据。

关键词: 卷叶黄精, 根际细菌, 解磷能力, 培养条件

Abstract: To address the current status of soil phosphorus deficiency， this study aimed to develop novel， safe， and efficient microbial fertilizers. Polygonatum cirrhifolium from Tibet was used as the test material， and the streak plate method was employed to isolate and screen phosphorus-solubilizing strains. Their phosphorus-solubilizing capacity was determined， and the culture conditions were investigated. The results showed that a total of 9 phosphorus-solubilizing bacterial strains were isolated. Among them， 5 strains with a phosphate solubilization index （PSI）>2.0 were screened， with phosphorus-solubilizing capacities ranging from 29.603 to 41.730 mg·L^-1. The strains were identified as follows： JYHJ-1 as Pseud-omonas yamanorum， JYHJ-2 as Pseudomonas koreensis， JYHJ-3 as Serratia proteamaculans， JYHJ-4 as Pseudomonas azotoformans， and JYHJ-5 as Microbacterium oxydans. Strain JYHJ-3 exhibited the strongest phosphorus-solubilizing ability， and it could secrete 7 types of organic acids as well as possess capabilities such as siderophore production， potassium solubilization， nitrogen fixation， and indole-3-acetic acid （IAA） production. In the single-factor experiments， the optimal phosphorus-solubilizing efficiency was achieved when lactose was used as the carbon source， ammonium acetate as the nitrogen source， initial pH was 5.5， inoculation volume was 2%， and NaCl concentration was 1%. Based on the single-factor experiments， response surface methodology （RSM） was applied to optimize the initial pH， inoculation volume， and NaCl concentration. The final optimal culture conditions were determined as follows： lactose as the carbon source， ammonium acetate as the nitrogen source， initial pH 5.5， inoculation volume 3%， and NaCl concentration 1%. In conclusion， the rhizosphere of Polygonatum cirrhifolium was rich in phosphorus-solubilizing bacterial resources. This study provided strain resources and a theoretical basis for the sustainable cultivation of medicinal Polygonatum cirrhifolium and the development of microbial fertilizers.

Key words: Polygonatum cirrhifolium, Rhizosphere bacteria, Phosphate-solubilizing capacity, Fermentation conditions

中图分类号:

S567.239

杨维, 马振林, 闫刚, 王泽, 岳辰, 刘航, 王瑞红, 张新军. 药用植物卷叶黄精根际解磷细菌的筛选鉴定及培养条件的优化[J]. 草地学报, 2026, 34(4): 1510-1523.

YANG Wei, MA Zhen-lin, YAN Gang, WANG Ze, YUE Chen, LIU Hang, WANG Rui-hong, ZHANG Xin-jun. Screening， Identification and Optimization of Culture Conditions of Phosphate-Solubilizing Bacteria in the Rhizosphere of Medicinal Plant Polygonatum cirrhifolium[J]. Acta Agrestia Sinica, 2026, 34(4): 1510-1523.

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药用植物卷叶黄精根际解磷细菌的筛选鉴定及培养条件的优化

Screening， Identification and Optimization of Culture Conditions of Phosphate-Solubilizing Bacteria in the Rhizosphere of Medicinal Plant Polygonatum cirrhifolium

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