复合促生菌剂发酵条件优化及其对青稞促生效果评价

doi:10.11733/j.issn.1007-0435.2022.01.026

草地学报 ›› 2022, Vol. 30 ›› Issue (1): 212-219.DOI: 10.11733/j.issn.1007-0435.2022.01.026

• 技术研发 • 上一篇

复合促生菌剂发酵条件优化及其对青稞促生效果评价

杨晓蕾¹, 李建宏¹, 姚拓¹, 陈建纲¹, 李琦¹, 张建贵¹, 王国基²

1. 甘肃农业大学草业学院/草业生态系统教育部重点实验室，甘肃兰州 730070;
2. 兰州苗乐农业科技发展有限公司，甘肃兰州 730070

收稿日期:2021-07-07 修回日期:2021-08-25 发布日期:2022-01-27
通讯作者: 李建宏, E-mail: lijianhong@gsau.edu.cn
作者简介:杨晓蕾(1997-)，女，甘肃兰州人，硕士研究生，主要从事微生物菌剂剂型研发及草地微生物多样性研究，E-mail: 1224728115@qq.com
基金资助:
国家重点研发计划(2019YFC0507703)；甘肃省科技计划项目(GFG2019-366-2)资助

Optimization of Fermentation Conditions of Three Growth Promoting Strains and Evaluation of Effects on Highland Barley

YANG Xiao-lei¹, LI Jian-hong¹, YAO Tuo¹, CHEN Jian-gang¹, LI Qi¹, ZHANG Jian-gui¹, WANG Guo-ji²

1. College of Grass Industry, Gansu Agricultural University/Key Laboratory of Grassland Ecosystem Education, Lanzhou, Gansu Province 730070, China;
2. Lanzhou Miaole Agricultural Technology Development Co., Ltd., Lanzhou, Gansu Province 730070, China

Received:2021-07-07 Revised:2021-08-25 Published:2022-01-27

摘要/Abstract

摘要： 为研制复合植物根际促生菌(Plant growth promoting rhizobacteria，PGPR)菌剂，提升菌体生物量，提高菌剂促生能力，本研究以3株分离自高寒草地的优良PGPR菌株组成复合菌系，通过单因素试验及响应面法Box-Behnken试验设计，优化复合菌系发酵培养基成分及培养条件，并通过盆栽试验评价优化前后菌剂对青稞(Hordeum vulgare var. nudum)的促生效果。结果表明：复合促生菌剂最适发酵培养基为甘油13 mL·L^-1、蛋白胨9 g·L^-1、酵母粉5 g·L^-1和碳酸钙5 g·L^-1；最适培养条件为pH=7.0、装液量30 mL(每150 mL三角瓶)、接种量10 %和摇床转速220 r·min^-1。此配方下菌剂活菌数达4.6×10¹³cfu·mL^-1，较原始配方活菌数提高148.39倍。与优化前相比，优化后复合促生菌剂使青稞的株高、鲜重、叶绿素含量、总根长、根表面积及根体积分别提高了13.05%, 22.83%, 47.74%, 73.21%, 93.09%和161.54%，促生效果显著。研究结果为复合促生菌剂的开发奠定了基础。

关键词: PGPR, 复合菌系, 培养基优化, 培养条件优化, 促生效果

Abstract: In order to develop a compound plant rhizosphere growth-promoting strains agent, increase the biomass of the strains and improve the growth-promoting ability of the strains. Here, three PGPR strains isolated from alpine grassland formed as a compound strain-agent. Through single factor test and Box-Behnken design, the composition of fermentation medium and cultural conditions of the compound strain-agent was optimized, and pot experiment was conducted to evaluate the growth promotion effects of microbial agents on highland barley before and after optimization. The results showed that the optimal fermentation medium for the compound growth-promoting strains agent was glycerol 13 mL·L^-1, peptone 9 g·L^-1, yeast powder 5 g·L^-1 and calcium carbonate 5 g·L^-1; the culture condition is pH=7.0, liquid volume 30 mL (per 150 mL erlenmeyer flask), inoculation volume 10%, and shaker rotation speed 220 r·min^-1. The number of viable strains in this formulation reached 4.6×10¹³ cfu·mL^-1, which was 148.39 times higher than that of the original formula. Compared with before optimization, the compound growth-promoting agent after optimization increased the plant height, fresh weight, chlorophyll content, total root length, root surface area and root volume of highland barley by 13.05%, 22.83%, 47.74%, 73.21%, 93.09% and 161.54%, the growth-promoting effect was significant. The results have laid the foundation for the development of the compound growth-promoting strains agent.

Key words: PGPR, Mixed strains, Medium optimization, Culture conditions, Promoting effect

中图分类号:

S144

杨晓蕾, 李建宏, 姚拓, 陈建纲, 李琦, 张建贵, 王国基. 复合促生菌剂发酵条件优化及其对青稞促生效果评价[J]. 草地学报, 2022, 30(1): 212-219.

YANG Xiao-lei, LI Jian-hong, YAO Tuo, CHEN Jian-gang, LI Qi, ZHANG Jian-gui, WANG Guo-ji. Optimization of Fermentation Conditions of Three Growth Promoting Strains and Evaluation of Effects on Highland Barley[J]. Acta Agrestia Sinica, 2022, 30(1): 212-219.

0
/ / 推荐

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://manu40.magtech.com.cn/Jweb_cdxb/CN/10.11733/j.issn.1007-0435.2022.01.026

https://manu40.magtech.com.cn/Jweb_cdxb/CN/Y2022/V30/I1/212

参考文献

[1] 中国国家标准化管理委员会. GB 20287-2006农用微生物菌剂[S]. 北京: 中国标准出版社, 2006: 4-16
[2] 李琦, 杨晓玫, 张建贵, 等. 农用微生物菌剂固定化技术研究进展[J]. 农业生物技术学报, 2019, 27(10): 1849-1857
[3] 刘丹丹, 李敏, 刘润进. 我国植物根围促生细菌研究进展[J]. 生态学杂志, 2016, 35(3): 815-824
[4] 李琦, 姚拓, 杨晓玫, 等. 半干旱地区不同剂型微生物菌肥替代部分化肥对燕麦生长和品质的影响[J]. 干旱区资源与环境, 2020, 34(3): 159-165
[5] LIU X X, LU R X, WANG X L. Screening indentification and growth-promoting effects of inorganic phosphorus-resolving strains of white clover rhizosphere[J]. Agricultural Science & Technology, 2012, 13(10): 2058-2064
[6] FOLMAN L B, POSTMA J, VEEN J A. Ecophysiological characterization of rhizosphere bacteria communities at different root locations and Plant Developmental stages of cucumber grown on rock wool[J]. Microbial Ecolohy, 2001, 4(2): 586-597
[7] 花玉鹏, 文才艺, 刘伟成, 等. 响应曲面法优化利迪链霉菌A02发酵培养基[J]. 中国生物防治学报, 2011, 27(4): 520-527
[8] 朱海云, 马瑜, 柯杨, 等. 抗猕猴桃细菌性溃疡病蜡样芽孢杆菌MA23培养基及发酵条件优化[J]. 中国农学通报, 2021, 37(7): 112-118
[9] 张雷, 张蕾, 王玲莉, 等. γ-聚谷氨酸生产菌株的鉴定及发酵培养基优化[J]. 食品工业科技, 2020, 41(20): 64-71
[10] 赵春海, 阚振荣, 安卫红, 等. α-乙酰乳酸脱羧酶产生菌发酵培养基碳源与氮源的优化[J]. 河北大学学报(自然科学版), 2005(03): 313-317, 322
[11] 肖怀秋, 李玉珍. 微生物培养基优化方法研究进展[J]. 酿酒科技, 2010(1): 90-94
[12] 周家萍, 孟梦, 夏国强. 中国拟青霉液体深层发酵条件的优化[J]. 食品研究与开发, 2021, 42(1): 172-176
[13] MAHDINIA E, DEMIRCI A, BERENJIAN A. Optimization of Bacillus subtilis natt growth parameters in glycerol-based medium for vitamin K (Menaquinone-7) production in biofilm reactors[J]. Bioprocess and Biosystems Engineering, 2018, 41(2): 195-204
[14] LI R F, XU Y. Fermentation optimization to improve production of antagonistic metabolites by Bacillus subtilis strain BS501a[J]. Journal of Central South University of Technology, 2011, 18(4): 1047-1053
[15] 徐亚英, 黄晓梅, 谢丽. 枯草芽孢杆菌液体发酵条件优化探究[J]. 南方农业, 2020, 14(20): 139-142
[16] 刘芳, 廖先清, 周荣华, 等. 枯草芽孢杆菌BZJN1发酵培养基和培养条件的优化[J]. 湖北农业科学, 2019, 58(20): 93-96
[17] HASKETT T L, TKACZ A, POOLE P S. Engineering rhizobacteria for sustainable agriculture[J]. The ISME Journal, 2021, 15: 949-964
[18] 左肖肖, 陆利霞, 李壹, 等. 微生物高密度培养策略[J]. 生物加工过程, 2016, 14(3): 81-86
[19] 郑双凤, 谭石勇, 谭武贵, 等. 生防芽孢杆菌高密度发酵技术研究进展[J]. 湖南农业科学, 2017(3): 120-124
[20] 李言郡, 葛红娟, 范恩宇, 等. 德氏乳杆菌WHH009高密度培养条件的研究[J]. 中国乳品工业, 2013, 41(5): 29-32
[21] 李伟. Bacillomycin D高产菌株的选育及其发酵工艺[D]. 南京: 南京农业大学, 2018: 60-66
[22] 刘芳, 廖先清, 周荣华, 等. 枯草芽孢杆菌BZJN1发酵培养基和培养条件的优化[J]. 湖北农业科学, 2019, 58(20): 93-96
[23] 朱海云, 马瑜, 柯杨, 等. 抗猕猴桃细菌性溃疡病蜡样芽孢杆菌MA23培养基及发酵条件优化[J]. 中国农学通报, 2021, 37(7): 112-118
[24] 许睿娉. 枯草芽孢杆菌HG-15菌体发酵全可溶培养基配方与条件优化[D]. 泰安: 山东农业大学, 2020: 24-27
[25] 周静, 黄文茂, 秦利军, 等. 四株PGPR菌株混菌发酵体系的构建及促生效应评价[J]. 生物技术通报, 2021, 37(4): 116-126

复合促生菌剂发酵条件优化及其对青稞促生效果评价

Optimization of Fermentation Conditions of Three Growth Promoting Strains and Evaluation of Effects on Highland Barley

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 1

编辑推荐

Metrics

本文评价