高羊茅赤霉素2-氧化酶编码基因的克隆及功能验证

doi:10.11733/j.issn.1007-0435.2012.01.021

›› 2012, Vol. 20 ›› Issue (1): 130-138.DOI: 10.11733/j.issn.1007-0435.2012.01.021

高羊茅赤霉素2-氧化酶编码基因的克隆及功能验证

徐光远¹, 刘松虎³, 梁本国³, 马磊², 杨佑明¹, 董江丽²

1. 中国农业大学农学与生物技术学院, 北京100193;
2. 中国农业大学生物学院, 北京100193;
3. 河南信阳农业高等专科学校园艺系, 河南信阳464000

收稿日期:2011-05-13 修回日期:2011-11-28 出版日期:2012-02-15 发布日期:2012-07-06
作者简介:徐光远(1987- ),男,山东潍坊人,硕士研究生,主要从事植物分子育种研究,E-mail:xuguangyuan137064@163.com
基金资助:
国家863计划项目“林果花草分子育种与品种创制”(2011AA100209)资助

Molecular Cloning and Characterization of Giberrellin 2-Oxidase Gene from Festuca arundinacea

XU Guang-yuana¹, LIU Song-hua³, LIANG Ben-guo³, MA Lei², YANG You-ming¹, DONG Jiang-li²

1. College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, China;
2. College of Biological Sciences, China Agricultural University, Beijing 100193, China;
3. Horticulture Department, Xinyang Agricultural College, Xinyang, Henan Province, 464000, China

Received:2011-05-13 Revised:2011-11-28 Online:2012-02-15 Published:2012-07-06

摘要/Abstract

摘要： 赤霉素(GA)是一类重要的植物激素,在植物的生长发育过程中发挥着重要作用。许多赤霉素代谢途径关键酶的编码基因已经被克隆和研究,但在高羊茅(Festuca arundinacea)中尚未见报道,利用RACE技术获得了高羊茅赤霉素2-氧化酶基因(FaGA2ox)。结果表明:FaGA2oxcDNA全长1566bp,包含1026bp开放阅读框,编码一个由341个氨基酸组成的蛋白质。FaGA2ox转基因烟草(Nicotiana tabocum)表现出赤霉素缺陷的表型,叶片面积明显变小,且叶片褶皱,叶片颜色暗绿,节间长度明显变短。综上所述,FaGA2ox在烟草中的过量表达可以导致植株矮化,为进一步利用该基因转化高羊茅培育矮化植株奠定基础。

关键词: 高羊茅, 矮化, 赤霉素2-氧化酶

Abstract: Gibberellins(GA),a class of important phytohormones,play critical roles in a variety of growth and developmental processes.Many genes encoding key enzymes in gibberellin metabolism have been identified,while there are no reports in Festuca arundinacea.The gibberellins 2-oxidase gene(FaGA2ox)is cloned by RACE from Festuca arundinacea.Full-length cDNA of FaGA2ox is 1566 bp,containing an open reading frame of 1026 bp sequence,encoding a protein of 341 amino acids.The GA-deficient phenotypes are caused by over-expression of FaGA2ox in transgenic tobacco(Nicotiana tabocum).Leaves become significant small with dark green and more wrinkle while the inter-node length becomes short.All these results indicate that over-expression of FaGA2ox gene inhibits tobacco growth.This study provides a foundation for breeding dwarfed Festuca arundinacea.

Key words: Festuca arundinacea, Dwarfed, Gibberellins 2-oxidase

中图分类号:

S543
Q943.2

徐光远, 刘松虎, 梁本国, 马磊, 杨佑明, 董江丽. 高羊茅赤霉素2-氧化酶编码基因的克隆及功能验证[J]. , 2012, 20(1): 130-138.

XU Guang-yuana, LIU Song-hua, LIANG Ben-guo, MA Lei, YANG You-ming, DONG Jiang-li. Molecular Cloning and Characterization of Giberrellin 2-Oxidase Gene from Festuca arundinacea[J]. , 2012, 20(1): 130-138.

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高羊茅赤霉素2-氧化酶编码基因的克隆及功能验证

Molecular Cloning and Characterization of Giberrellin 2-Oxidase Gene from Festuca arundinacea

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