草地学报

›› 2006, Vol. 14 ›› Issue (2): 124-128.DOI: 10.11733/j.issn.1007-0435.2006.02.006

• 研究论文 • 上一篇    下一篇

高羊茅耐寒突变体的诱发与鉴定

张彦芹1, 贾炜珑3, 杨丽莉1, 郭先龙1, 吴锜2, 胡鸢雷2, 林忠平2, 王淼4, 许隆君4   

  1. 1. 山西省农业科学院旱地农业研究中心, 太原, 030031;
    2. 天津大学理学院, 天津, 300072;
    3. 北京大学生命学院, 北京, 100871;
    4. 山西大学生科院, 太原, 0300061
  • 收稿日期:2006-03-28 修回日期:2006-05-22 出版日期:2006-05-15 发布日期:2006-05-15
  • 作者简介:张彦芹(1964- ),女,助研,主要从事草类辐射育种及农业生物技术研究,E-mail:zyq20047@yahoo.com.cn
  • 基金资助:
    国家863项目(2001AA212161);省农科院高新技术项目(YGX0501)

Induction and Identification of Cold Tolerance for Festuca Arundinacea

ZHANG Yan-qin1, JIA Wei-long3, YANG Li-li1, GUO Xian-long1, WU Qi2, HU Yuan-lei2, LIN Zhong-ping2, WANG Miao4, XU Long-jun 4   

  1. 1. Arid Farming Research Center, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China;
    2. Life Science College, Beijing University, Beijing, 100871, China;
    3. School of science of Tianjin University, Tianjin, 300072;
    4. School of Life Science and Technology of Shanxi University, Taiyuan 030006, China
  • Received:2006-03-28 Revised:2006-05-22 Online:2006-05-15 Published:2006-05-15

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摘要： 以高羊茅爱瑞3号品种(Festuca arundinacea Schred.cv.Airea Ⅲ)为材料,以60Co-r射线照射种子和分化苗。结果表明,在辐射当代选出耐寒突变体ARF 001-12、ARG 001-17和ARG 001-16,经电解质外渗率、自然低温条件下束缚水和叶绿素含量测定,其耐寒性明显优于对照,叶根比小于对照,单株生物量低于对照,可作为新的耐寒种质利用;M0、M1代RAPD分析结果表明,其DNA已发生变异。

关键词: 高羊茅, 辐射诱变, 突变体, 耐寒性

Abstract: Using 60Co-γ-ray to irradiate dry seeds and differential seedling of material of Festuca arundinacea Schreb Airei III,3 cold-resistance mutants（ARF001-12,ARG001-17,and ARG001-16） were selected from the irradiated generation. The 3 mutants with cold-resistance characters are apparently better than F.arundinacea Schreb Airei III in the test of electrolyte osmotic ratio under synthetic low temperature,and the content of bound-water and chlorophyll under natural low temperature.The testing result of single plant's biomass and leaf-rootratio indicated that the 3 mutants had lower leaf-root-ratio and smaller biomass,which was conductive to frigid resistance character's expression.The results demonstrated these mutants could be used as new cold-resistance seeding resources.Furthermore,mutant variations were found at DNA level through the RAPD analyzing for M0、M1 generation.

Key words: Festuca arundinacea Schreb, Induced mutation by radiation, Mutant, Cold tolerance

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