Abstract: Experiments were conducted to determine the germination rate, electrical conductivity,glucose content, SOD isoenzyme and ATP content of seed leachates during seed germination in ageing ornon-ageing tall fescue seed.The results showed that germination rate and germination speed decreasedwith the increasing of accelerated ageing time in tall fescue seed, and germination speed decreased fasterthan germination rate, the electrical conductivity and glucose content of seed leachates in ageing seed werehigher than those in non-ageing seed, and electrical conductivity gradually increaed with the extendingageing time.New pattems of SOD isoenzyme, which formed by stimulation of lightly accelerated ageing,could prevent the damage of membrane system.ATP content of ageing seed obviously decreased duringgermination contrasting with non-ageing seed.ATP content was high in seriously ageing seed during earlygermination due to the ATP utilization was limited, but ATP content decreased rapidly with the prolong ofthe germination

Key words: Tall fescue seed, Accelerated ageing, Physiological and biochemical change

摘要： 本研究对未老化与加速老化处理的高羊茅种子进行发芽率、浸出滚电导率、葡萄糖含量、超氧化物歧化酶(SOD)同工酶、发芽过程中ATP含量的测定结果表明,高羊茅种子随着老化时间的增加,发芽率和发芽势明显下降,而发芽势下降速度远大于发芽率。老化种子浸出液的电导率明显高于对照,并随着老化时间的延长,浸出液的电导率逐渐增加,浸出液中葡萄糖含量显著高于对照。轻微老化可刺激高羊茅种子中超氧化物歧化酶(SOD)同工酶出现新的酶带,防止膜系统受到破坏。与对照相比,老化种子在发芽过程中ATP含量均明显地降低,严重老化种子在发芽前期因ATP利用受到限制,ATP含量较高,但随着发芽的延续含量则大幅度降低。

关键词: 高羊茅种子, 人工加速老化, 生理生化变化