›› 2002, Vol. 10 ›› Issue (4): 274-278.DOI: 10.11733/j.issn.1007-0435.2002.04.007

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Studies on RAPD Polymorphic in Alfalfa Resistant to Brown Blot

GUI Zhi1, YUAN Qing-hua2   

  1. 1. Tianjin Agricultural College, Tianjin 300384, China;
    2. Institute of Animal Science, CAAS, Beijing 100094, China
  • Received:2002-02-28 Revised:2002-05-16 Online:2002-11-15 Published:2002-11-15

抗褐斑病苜蓿RAPD多态性研究

桂枝1, 袁庆华2   

  1. 1. 天津农学院农学系, 天津, 300384;
    2. 中国农业科学院畜牧研究所, 北京, 100094
  • 通讯作者: 袁庆华
  • 作者简介:桂枝(1973- ),女,山西人,助教,1997年本科毕业于山西师范大学生物系,2001年于甘肃农业大学获得硕士学位,现在天津农学院从事教学与科研工作.
  • 基金资助:
    国家自然科学基金(编号:39870559)资助项目

Abstract: Randomly amplified polymorphic DNA (RAPD) was employed to detect molecular markers linked to the gene resistant of brown blot in 5 alfalfa varieties. The results show that, 54 of 107 arbitrary primers could amplify clear and repeating bands; polymorphic bands were found between resistant and susceptible plants of more than 3 varieties of alfalfa,that were RAPD linked to the gene resistant of brown blot;the length of the bands was 600~1400bp.

Key words: Alfalfa, Brown blot, RAPD markers

摘要: 运用RAPD技术,采用集群分离分析法对5个苜蓿品种进行抗褐斑病基因连锁的分子标记研究。结果表明,在160个随机引物中有107个有扩增产物,其中54个扩增出的产物的电泳图谱较稳定、清晰,再从中筛选出8个能够同时在3个以上苜蓿品种内的抗、感材料间显示多态性的随机引物,其RAPD标记的大小为600~1400bp。

关键词: 苜蓿, 褐斑病, RAPD标记

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