Acta Agrestia Sinica

›› 2004, Vol. 12 ›› Issue (4): 276-280.DOI: 10.11733/j.issn.1007-0435.2004.04.005

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Phenotype Diversities of Rhizobia Isolated from Different starins of Medicago sativa

WAN Xiao-hong1, WEI Ge-hong1,2, YANG Ya-zhen1, ZHU Yu-hua1   

  1. 1. College of Resources and Environment, Northwest Agriculture and Forestry Sci-Tech University, Yangling, Shanxi Province 712100, China;
    2. Key State Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Yangling, Shanxi Province 712100, China
  • Received:2003-07-03 Revised:2004-03-03 Online:2004-11-15 Published:2004-11-15

紫花苜蓿品种根瘤菌表型多样性研究

万晓红1, 韦革宏1,2, 杨亚珍1, 朱毓华1   

  1. 1. 西北农林科技大学资源环境学院, 陕西, 杨凌, 712100;
    2. 黄土高原土壤侵蚀与旱作农业国家重点实验室, 陕西, 杨凌, 712100
  • 通讯作者: 韦革宏,E-mail:weigehong@yahoo.com.cn
  • 作者简介:万晓红(1978- ),女,硕士研究生,主要从事根瘤菌资源多样性研究,wanxiaohonggg@163.net;韦革宏,通讯作者Authorforcorrespondenceweigehong@yahoo.com.cn
  • 基金资助:
    陕西省农业攻关项目(2003K02-G16),教育部全国优秀博士学位论文专项基金(200254)和高等学校优秀青年教师教学科研奖励计划

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Abstract: A study of 113 phenotype features, including plant-growing temperature, nutrition utilization, antibiotic resistance, and NaCl and alkali tolerance, of 52 strains of Medicago sativa, isolated from the rhizobia of 22 M.sativa strains and 34 representative strains, was conducted. The result shows that the three strains:NWMSH022, NWMSH023, and NWMSH028 boast stronger NaCl and alkali tolerance than the others. By the analysis of MINTS software, a dendrogram of numerical taxonomy shows that, except NWMSH009, and NWMSH023, all the examined strains cluster into one phenotype at a similarity level of 89%. It takes a further genetic study to determine whether this new phenotype differs from the known rhizobial species.

Key words: Grassland science, Medicago sativa, Rhizobium, Phenotype diversity

摘要： 选用2株分离自22个紫花苜蓿品种的根瘤菌和34株已知参比菌株,进行生长温度、营养利用、抗生素抗性和耐逆性等113个表型性状研究,结果表明:NWMSH022、NWMSH023和NWMSH028这3株菌抗逆性较强;通过MINTS软件分析,得到数值分类树状图;在89%的相似水平上,除NWMSH009和NWMSH023,供试未知菌株聚为不含已知参比菌株的新表观群,该表观群是否为新属种有待进行基因研究.

关键词: 草原学, 紫花苜蓿, 根瘤菌, 表型多样性

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