RAPD Analysis of Elymus canadensis,E.excelsus and their Hybrid F1

doi:10.11733/j.issn.1007-0435.2008.01.005

›› 2008, Vol. 16 ›› Issue (1): 23-27.DOI: 10.11733/j.issn.1007-0435.2008.01.005

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RAPD Analysis of Elymus canadensis,E.excelsus and their Hybrid F₁

LI Xiao-lei, YU Zhuo, HE Peng-cheng, MA Yan-hong, LI Zao-zhe

College of Agronomy, Inner Mongolia Agriculture University, Hohhot, Inner Mongolia Autonomous Region, 010019, China

Received:2007-07-06 Revised:2007-09-03 Online:2008-02-15 Published:2008-02-15

加拿大披碱草、肥披碱草及其杂种F₁ RAPD分析

李小雷, 于卓, 贺鹏程, 马艳红, 李造哲

内蒙古农业大学农学院, 呼和浩特, 010019

通讯作者: 于卓, E-mail:yuzhuo58@sina.com
作者简介:李小雷(1980- ),黑龙江绥化厂,博士研究生,研究方向为饲用作物遗传育种;
基金资助:
国家自然科学基金(30560100);内蒙古自然科学基金(200607010505)资助项目

Abstract

Abstract: The genetic diversity of Elymus canadensis L.,E.excelsus Turcz.,and their hybrid F₁ were analyzed by the RAPD molecular marker technology.Total 215 loci were amplified from 16 primers out of 153 random primers,the polymorphic loci percentage were 72.1%.The no.of band per primer ranged from 4.3 to 4.8,and the fragment length of the amplified products was from 100 to 2000 bp.The genetic distance between E.canadensis and E.excelsus reached to 0.7122,while hybrid F₁ was closer to to male E.excelsus(0.2329) than female E.canadensis(0.6643),and hybrid F₁ leaned to E.excelsus.These results could be used as foundation for the fertility restoration and utilization of the hybrid of E.canadensis and E.excelsus.

Key words: Elymus canadensis L., Elymus excelsus Turcz., Interspecific hybrid, Genomic DNA, RAPD

摘要： 利用RAPD技术对加拿大披碱草(Elymus canadensis L.)和肥披碱草(Elymus excelsus Turcz.)及其杂种F₁的遗传差异性进行研究,从153个随机引物中筛选出16个适宜引物,共扩增出215个RAPD位点,多态性位点比率为72.1%,平均每条引物的多态性位点为4.3-4.8个,DNA片段长度在100-2000 bp之间。加拿大披碱草与肥披碱草的遗传距离较大(0.7122),杂种F₁与父本肥披碱草的遗传距离较近(0.2329),而与母本加拿大披碱草相对较远(0.6643),杂种F₁更偏向其父本。该结果可为加拿大披碱草-肥披碱草杂种后代的育性恢复和选育利用提供依据。

关键词: 加拿大披碱草, 肥披碱草, 种间杂种, 基因组DNA, RAPD

CLC Number:

Q943

LI Xiao-lei, YU Zhuo, HE Peng-cheng, MA Yan-hong, LI Zao-zhe. RAPD Analysis of Elymus canadensis,E.excelsus and their Hybrid F₁[J]. , 2008, 16(1): 23-27.

李小雷, 于卓, 贺鹏程, 马艳红, 李造哲. 加拿大披碱草、肥披碱草及其杂种F₁ RAPD分析[J]. , 2008, 16(1): 23-27.

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RAPD Analysis of Elymus canadensis,E.excelsus and their Hybrid F₁

加拿大披碱草、肥披碱草及其杂种F₁ RAPD分析

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