Abstract: One group of dry seeds of Medicago sativa× Melilotoides ruthenicus(L.) Sojak cv.Longmu 803 was irradiated by mixed high energy particle field with five dosages,i.e.109Gy,145Gy,195Gy,284Gy,and 560Gy,which was generated from E2 beam lines of LINAC of Beijing Electron Positron Collider;the other equal group was irradiated with the same five dosages by the ⁶⁰Co γ-rays.The different bioeffects were mutually studied between the different processing meuhods for a given dosage and among the different dosages for a given processing method.The results show the seeds in mixed high-energy particle field irradiated group had significantly higher germination energy and germination rate than those in γ-rays irradiated group,and their germination energy was higher but germination rate lower than those of CK;the order of micronucleus rate was γ-rays irradiate group>mixed high-energy particle field irradiated group>CK;the plant height and weight in ⁶⁰Co γ-rays group were decreased along with the increased dosages and lower than those in mixed high-energy particle field irradiated group and CK with exception at dosage of 109Gy.The mixed high-energy particle field irradiation had lighter damage effects than γ-rays irradiation on plants.

Key words: Alfalfa, Mixed high-energy particle field, ⁶⁰Co γ-rays, Induced mutation, Biological effect

摘要： 分别利用5个不同剂量(109、145、195、284和560Gy)的高能混合粒子场和⁶⁰Coγ射线处理龙牧803紫花苜蓿(Medicago sativa L.×Melilotoides ruthenicus (L.) Sojak cv. Longmu 803)干种子,比较同一剂量不同处理方法间和同一处理方法不同剂量间的生物学效应。结果表明,高能混合粒子场处理的种子发芽势和发芽率明显高于γ射线处理,发芽势高于对照,发芽率低于对照;微核率略低于γ射线处理,两者均显著高于对照;γ射线处理的株高和产量随着处理剂量的增高而降低,除109Gy外,均低于对照和高能混合粒子场处理,高能混合粒子场处理对植株的损伤效应较小。

关键词: 紫花苜蓿, 高能混合粒子场, γ射线, 诱变, 生物学效应