Screening of High Efficient Symbiotic Rhizobium for Zhongmu No.1 Alfalfa

doi:10.11733/j.issn.1007-0435.2008.05.013

›› 2008, Vol. 16 ›› Issue (5): 497-500.DOI: 10.11733/j.issn.1007-0435.2008.05.013

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Screening of High Efficient Symbiotic Rhizobium for Zhongmu No.1 Alfalfa

KANG Jun-mei¹, ZHANG Li-juan¹, GUO Wen-shan¹, YANG Qing-chuan¹, WEI Dou-xing²

1. Institute of Animal Sciences, CAAS, Beijing 100193, China;
2. Grassland Station of Haixi, Qinghai Province 817000, China

Received:2007-09-04 Revised:2008-06-05 Online:2008-10-15 Published:2008-10-15

中苜1号紫花苜蓿高效共生根瘤菌的筛选

康俊梅¹, 张丽娟¹, 郭文山¹, 杨青川¹, 魏窦兴²

1. 中国农业科学院北京畜牧兽医研究所, 北京, 100193;
2. 青海省海西州草原站, 青海海西州, 817000

通讯作者: 杨青川,E-mail:qchyang66@yahoo.com.cn
作者简介:康俊梅(1972- ),女,内蒙古集宁人,助研,在读博士,主要从事牧草与草坪草遗传育种工作,E-mail:kangjmei@yahoo.com.cn
基金资助:
中国农业科学院北京畜牧兽医研究所基本科研业务费专项基金创新团队(ywf-td-3)

Abstract

Abstract: The effects of inoculation with 8 rhizobial strains on Medicago sativa L.cv.Zhongmu No.1 were studied.The results show that there was significant difference in different strains for alfalfa growth improvement.The shoot dry weight of Zhongmu No.1 inoculated with rhizobia increased 3.8%～26.12%,the number of nodules increased 2.9%～41.43%,the root dry weight increased 5.8%～41.38%,and the content of crude protein increase 2.39%～8.5% in comparison with the control.Zhongmu No.1 grew well by inoculation with the strain ACCC17544 which enhanced the symbiotic nitrogen fixation,forage yield,and protein content in alfalfa.

Key words: Rhizobium, Alfalfa(Medicago sativa L.), Nitrogen fixation

摘要： 研究8株根瘤菌对"中苜1号"紫花苜蓿(Medicago sativa L.cv.Zhongmu No.1)的接种效果。结果表明:8株根瘤菌接种效果差异明显;接种后干草重、根瘤数、干根重、粗蛋白质含量比对照分别增加了3.8%~26.12%、2.9%~41.43%、5.8%~41.38%和2.39%~8.5%;筛选出与中苜1号最佳共生匹配的根瘤菌ACCC17544,该菌株能增强其共生固氮效果,既提高干草产量,又增加粗蛋白质含量,具有一定的生产应用价值。

关键词: 根瘤菌, 紫花苜蓿, 固氮

CLC Number:

S541

KANG Jun-mei, ZHANG Li-juan, GUO Wen-shan, YANG Qing-chuan, WEI Dou-xing. Screening of High Efficient Symbiotic Rhizobium for Zhongmu No.1 Alfalfa[J]. , 2008, 16(5): 497-500.

康俊梅, 张丽娟, 郭文山, 杨青川, 魏窦兴. 中苜1号紫花苜蓿高效共生根瘤菌的筛选[J]. , 2008, 16(5): 497-500.

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References

[1] 沈世华,荆玉祥.中国生物固氮研究现状和展望[J].科学通报,2003,48(6):532-540
[2] 林稚兰,黄秀梨.现代微生物学与实验技术[M].北京:科学出版社,2000.9-13
[3] Zabran H H.Rhizobium-legume symbiosis and nitrogen fixation under severe condition and in arid climate[J].Mierobiol Mol.Biol.Rev.,1999,63:968-989
[4] Denarie J,Roche P.Rhizobium nodulation signals[A].In:Verma D P S,ed.Molecular signals in plant-microbe communications[C].London:CR(" Press,1991.296-324
[5] 曾昭海.紫花苜蓿高效根瘤菌筛选及田间鉴定方法研究[D].中国农业大学博十学位论文,2003
[6宁国赞,刘慧琴,马晓彤.中国豆科牧草根瘤菌资源的采集宝藏及利用[J].草地学撤,1999,7(2):165-172
[7] 戴小密,刘彦杰.叶小梅,等.接种大豆根瘤菌(Sinorhizobiumfredii)遗传丁程菌株LMG101对大豆的增产效应[J].中国农业科学,2003,36(1):66-70
[8] Gibson A H,Curnow B Co Bergersen F J,et al.Studies of strains of Rhizobium tri folii associated with Tri folium subterraneum L.pastures in south-easatern Australia[J].Soil Biology and Biochemistry,1975,7:95-102
[9] 陈丹明,曾昭海,隋新华,等.紫花苜蓿高效共生根瘤菌的筛选[J].草业科学,2002,19(6)27-31
[10] Hardarson G,Heichel G H,Barnes D K.Rhizobiat strain preference of alfalfa populations selected for characteristics associated with N2 fixation[J].Crop.Sci.,1982,22:55-58
[11] Brockwell J,Bottomley P J.Recent advances in inoeulant technology and prospects for the future[J] ,Soil.Biol.Biochem.,1995,27:683-697
[12] Kosslak R M,Bohlool B B.Influence of environmental factors on interstrain competition in Rhizobium japonicm[J].Appl.Environ.Microbiol.,1985,49:1128-1133
[13] 陈文新,李阜棣,阎章才.我国土壤微生物学和生物同氮研究的回顾与展望[J].院士论坛.世界科技研究与发展,2002,4:6-12

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[15]	NIU Jian-zhong, ZHOU He, SHI De-kuan. Effect of Moisture Content,Degree of Bale Compactness, and Urea Preservation on Baled Alfalfa Hay Quality [J]. , 2006, 14(1): 34-38.

Screening of High Efficient Symbiotic Rhizobium for Zhongmu No.1 Alfalfa

中苜1号紫花苜蓿高效共生根瘤菌的筛选

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