Abstract: Through the analysis of isozyme zymograms of peroxidase(POD),esterase(EST),superoxide dismutase(SOD),and polyphenol oxidase(PPO),the zymogram characteristics and relationship of Elytrigia hybrids,E. elongate×E. intermedia,and their parents were studied.The results are as follows: the band number,migration rate,and pigmentation degree of isoenzymes between parents had slight differences and their genetic relationship could be close.F₁ hybrids lacked of some bands of POD,SOD,and PPO isoenzyme as compared to their parents,but had some new bands in descendant of reciprocal cross;EST bands of hybrid F₁ were almost the same as the parents except the pigmentation degree.The characteristic of isozyme bands of POD and PPO of hybrid F₁ had the tendency to E.elongata,and the SOD isozyme band to E.intermedia.Isozyme Polymorphisms existed in hybrid F₁ which could be used as the genetic marker to identify hybrids and determine the plants with target characteristics in descendant strains.

Key words: Elytrigia elongata(Host) Nevski, E.intermedia(Host) Nevski, Isoenzyme

摘要： 通过对过氧化物酶(POD)、酯酶(EST)、超氧化物歧化酶(SOD)和多酚氧化酶(PPO)同工酶谱的分析,探讨中间偃麦草(Elytrigia elongata(Host)Nevski)、长穗偃麦草(E.intermedia(Host)Nevski)及其杂种F₁代的酶谱特征和亲缘关系。结果表明:亲本同工酶酶带数目、迁移率、着色程度等差异不大,二者亲缘关系较近。与亲本相比,杂种F₁代存在POD、SOD、PPO酶带丢失现象,但正、反交植株中均产生了一些亲本没有的新生带。杂种F₁代EST酶谱与亲本基本相同,仅着色程度略有差异。杂种F₁代植株的POD、PPO酶谱特征有偏向中间偃麦草的倾向,而SOD酶谱特征倾向于长穗偃麦草。杂种F₁代同工酶具有多态性,可作为遗传标记用于杂种鉴定和后代株系目标性状的选择。

关键词: 中间偃麦草, 长穗偃麦草, 同工酶