›› 2009, Vol. 17 ›› Issue (4): 452-455.DOI: 10.11733/j.issn.1007-0435.2009.04.008

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Creation and Identification of ‘Chinese Spring’ wheat Elytrigia elongata Translocation Lines

Liu Feng-qi1, ZHANG Yue-xue1, TANG Feng-lan1, LI Ji-lin2, XU Xiangling2   

  1. 1. Institute of Pratacultural Sciences, Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang Province 150086, China;
    2. Biology Department, Life and Environment Science College, Harbin Normal University, Harbin, Heilongjiang Province 150080, China
  • Received:2009-02-18 Revised:2009-06-12 Online:2009-08-15 Published:2009-08-15

“中国春”小麦-偃麦草易位系的创制与鉴定

刘凤歧1, 张月学1, 唐凤兰1, 李集临2, 徐香玲2   

  1. 1. 黑龙江省农业科学院草业研究所, 黑龙江, 哈尔滨, 150086;
    2. 哈尔滨师范大学生命与环境科学学院生物系, 黑龙江, 哈尔滨, 150025
  • 作者简介:刘凤歧(1982- ),男,黑龙江克山人,硕士,实习研究员,从事牧草细胞遗传学与育种研究,E-mail:l_fq2003@163.com
  • 基金资助:
    国家自然科学基金资助项目(No.39870417)

Abstract: The ‘Chinese Spring’ wheat (Triticum aestivum L.)-Elytrigia elongata translocation lines were created by hybridizing ‘Chinese Spring’ wheat-Elytrigia elongata 2E disomic addition line with,the method of gametocidal chromosome creating translocation line,the ‘Chinese Spring’ wheat-Ae. cylindrical 2C disomic addition line.The F1 generation had setting percentage of 31.02%,and it's inbreeding rate was 31.41%, which was much lower than 92.59% of the parental lines.9 inter-genomic translocations,involving Chinese Spring and E.elongata chromosomes,were screened out from 127F2 progenies by C-banding and genomic in situ hybridization (GISH).

Key words: E.elongata,Gametocidal chromosome, C-banding,In situ hybridization,Translocation lines

摘要: 利用杀配子染色体创制易位系的方法,以"中国春"小麦(Triticum aestivum L.)-长穗偃麦草(Elytrigia repens)(2E)异二体附加系为母本与"中国春"小麦-柱穗山羊草(Ae.cylindrical)(2C)异二体附加系为父本杂交,创制"中国春"小麦-偃麦草易位系.F1的结实率为31.02%,F1套袋自交结实率为31.41%,亲本自交结实率92.59%;获得的F2利用染色体C-分带和原位杂交进行鉴定,从127株后代中筛选出9株易位系.

关键词: 长穗偃麦草, 杀配子染色体, C-分带, 原位杂交, 易位系

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