Creation and Identification of ‘Chinese Spring’ wheat Elytrigia elongata Translocation Lines

doi:10.11733/j.issn.1007-0435.2009.04.008

›› 2009, Vol. 17 ›› Issue (4): 452-455.DOI: 10.11733/j.issn.1007-0435.2009.04.008

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Creation and Identification of ‘Chinese Spring’ wheat Elytrigia elongata Translocation Lines

Liu Feng-qi¹, ZHANG Yue-xue¹, TANG Feng-lan¹, LI Ji-lin², XU Xiangling²

1. Institute of Pratacultural Sciences, Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang Province 150086, China;
2. Biology Department, Life and Environment Science College, Harbin Normal University, Harbin, Heilongjiang Province 150080, China

Received:2009-02-18 Revised:2009-06-12 Online:2009-08-15 Published:2009-08-15

“中国春”小麦-偃麦草易位系的创制与鉴定

刘凤歧¹, 张月学¹, 唐凤兰¹, 李集临², 徐香玲²

1. 黑龙江省农业科学院草业研究所, 黑龙江, 哈尔滨, 150086;
2. 哈尔滨师范大学生命与环境科学学院生物系, 黑龙江, 哈尔滨, 150025

作者简介:刘凤歧(1982- ),男,黑龙江克山人,硕士,实习研究员,从事牧草细胞遗传学与育种研究,E-mail:l_fq2003@163.com
基金资助:
国家自然科学基金资助项目(No.39870417)

Abstract

Abstract: The ‘Chinese Spring’ wheat (Triticum aestivum L.)-Elytrigia elongata translocation lines were created by hybridizing ‘Chinese Spring’ wheat-Elytrigia elongata 2E disomic addition line with,the method of gametocidal chromosome creating translocation line,the ‘Chinese Spring’ wheat-Ae. cylindrical 2C disomic addition line.The F₁ generation had setting percentage of 31.02%,and it's inbreeding rate was 31.41%, which was much lower than 92.59% of the parental lines.9 inter-genomic translocations,involving Chinese Spring and E.elongata chromosomes,were screened out from 127F₂ progenies by C-banding and genomic in situ hybridization (GISH).

Key words: E.elongata,Gametocidal chromosome, C-banding,In situ hybridization,Translocation lines

摘要： 利用杀配子染色体创制易位系的方法,以"中国春"小麦(Triticum aestivum L.)-长穗偃麦草(Elytrigia repens)(2E)异二体附加系为母本与"中国春"小麦-柱穗山羊草(Ae.cylindrical)(2C)异二体附加系为父本杂交,创制"中国春"小麦-偃麦草易位系.F1的结实率为31.02%,F1套袋自交结实率为31.41%,亲本自交结实率92.59%;获得的F2利用染色体C-分带和原位杂交进行鉴定,从127株后代中筛选出9株易位系.

关键词: 长穗偃麦草, 杀配子染色体, C-分带, 原位杂交, 易位系

CLC Number:

S330.25

Liu Feng-qi, ZHANG Yue-xue, TANG Feng-lan, LI Ji-lin, XU Xiangling. Creation and Identification of ‘Chinese Spring’ wheat Elytrigia elongata Translocation Lines[J]. , 2009, 17(4): 452-455.

刘凤歧, 张月学, 唐凤兰, 李集临, 徐香玲. “中国春”小麦-偃麦草易位系的创制与鉴定[J]. , 2009, 17(4): 452-455.

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References

[1] 董玉琛,郑殿生.中国小麦遗传资源[M].北京:中国农业出版社,2000,183-185
[2] 薛秀庄,许喜堂,王样正.用染色体工程法培育小麦新品种[J].遗传学报,1992,19 (4)316-32
[3] 刘文轩,陈佩度,刘大钧.利用荧光原位杂交技术检测导入普通小麦的大赖草染色质.[J].遗传学报,1999,26:546-551
[4] Sears E R and Okamoto M.Intergenomic chromosome relationships in wheat[J].Proc.X Internat.Congr,Genet,Montreal.1958,2:258-259
[5] Sears E R.An induced mutant with homoeologous pairing in common wheat[J].Can J.Genet.Cytol.1977,19:585-593
[6] Sears E R.Sears L M S.The telocentric chromosomes of common wheat[J].In:Proc.of the 5th Int.Wheat Gent.Symp.New Delhi.1978,389-407
[7] 徐惠君,辛志勇,刘四新,等.组织培养与普通小麦异源易位系选育[J].遗传学报,1996,23 (5),376-381
[8] A Masoudi-Nejad,S Nasuda,T R Endo.Transfer of rye chromosome segments to wheat by a gametocidal system[J].Chromosome Research.2002,10:349-357
[9] 李集临,徐香铃,徐萍,等.利用中国春-山羊草2C二体附加系与中国春-偃麦草5E二体附加系杂交诱发染色体易位和缺失[J].遗传学报,2003,30:345-349
[10] 郭长虹,王同昌,李集临.杀配子染色体及其在小麦遗传和育种研究中的应用[J].生物工程进展,2000.20(1):67-69
[11] 李集临,王宁,郭东林,郭长虹等.小麦-黑麦染色体代换的研究[J].植物研究,2002,22(2):121-125
[12] 苑泽宁,徐香玲,李集临.小麦-黑麦代换系间杂交后代染色体易位的研究[J].植物研究,2004,24 (2):223-225
[13] ENDO T R.Complete identification of wheat chromosomes by means of a C-banding technique[J].J pn.J.Genet.1986,61:89-93
[14] 孙仲平,徐香玲,李集临,等.利用杀配子染色体2C诱导中国春-黑麦二体附加系染色体畸变的研究[J].遗传学报,2004,31 (11):1268-1274
[15] 吉万全,薛秀庄,王秋英,等.中间偃麦草的GISH分析.西北植物学报,2001,21:401-405
[16] 童一中,褚圻编译.小麦细胞遗传学[M].北京:农业出版社,1983,126-127
[17] Davies,P.A et al.Centric fusion between nonhomologous rye chromosomes in wheat[J].Can Genet.Cytol,1985,27:627-632
[18] Endo,T.R Structural changes of rye chromosome 1R induced by a gametocidal chromosome[J].Jpn.Genet,1994,69:13-19
[19] 曲敏,张延明,李集临,等.利用杀配子染色体2C诱导中国春-长穗偃麦草1E二体附加系染色体畸变的研究[J].食品科学,2007,28(10):342-348
[20] Endo T R,Tsunewaki K.Sterility of common wheatwith Ae -gilops triuncialis cytop lasm[J].Hered,1975,66:13-18
[21] 韩方普,李集临.小偃麦细胞遗传学[M].北京:中国农业科技出版社,1994:1-3
[22] 陈穗云,夏光敏,陈惠民,等.小麦与高冰草(长穗偃麦草)体细胞杂种株系[J].西北植物学报,2000,20:327-332
[23] 李玉京,刘建中,李振声,等.长穗偃麦草基因组中与耐低磷营养胁迫有关的基因的染色体定位[J].遗传学报,1999,26:703-710
[24] Endo TR.The gametocidal chromosome as a tool for chromosome manipulation in wheat[J].Chromosome Res,2007,15:67-75

Creation and Identification of ‘Chinese Spring’ wheat Elytrigia elongata Translocation Lines

“中国春”小麦-偃麦草易位系的创制与鉴定

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