Acute Toxicity Analyses of Stellera chamaejasme Root in Mongolian Gerbils(Meriones unguiculatus)

doi:10.11733/j.issn.1007-0435.2010.05.022

›› 2010, Vol. 18 ›› Issue (5): 731-734.DOI: 10.11733/j.issn.1007-0435.2010.05.022

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Acute Toxicity Analyses of Stellera chamaejasme Root in Mongolian Gerbils(Meriones unguiculatus)

SHI Kun¹, ZHOU Da-sen¹, GUO Yong-wang², SHI Da-zhao¹, WANG Deng¹

1. College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China;
2. National Agro-tech Extensions and Service Center, Beijing 100026, China

Received:2010-06-03 Revised:2010-09-20 Online:2010-10-15 Published:2010-10-15

瑞香狼毒根对长爪沙鼠的急性毒力测试

史琨¹, 周大森¹, 郭永旺², 施大钊¹, 王登¹

1. 中国农业大学农学与生物技术学院, 北京, 100193;
2. 全国农业技术推广中心, 北京, 100026

通讯作者: 王登,E-mail:wangdeng@cau.edu.cn
作者简介:史琨(1989- ),女,山西平遥人,学士,E-mail:kellyfour@yeah.net
基金资助:
国家自然科学基金项目(30800727);全国农区统一灭鼠项目资助

Abstract

Abstract: Both whole Stellera chamaejasme and its extract are demonstrated to have powerful toxicity to some pest insects.The poison bait containing 17% roots of Stellera chamaejasme was used to test the acute toxicity of Stellera chamaejasme in Mongolian gerbils according to Sun′s improved Kou′s Integrated Way.Mongolian gerbils were treated with 5 treatments of toxicant bait according to pretested results: the lowest dose was 7.0 g/kg and the optimal gradient dose radio was 0.65.Results indicate that the body weight of treatment group was significantly decreased as compared to control(P=0.029<0.05).The rate of liver to body weight was significantly decreased in groupⅤ(P=0.014<0.05,whereas,the percent of kidney to body weight was slightly increased in groupⅤ(P=0.102>0.05) compared with controls.The LD₅₀ of Stellera chamaejasme in Mongolian gerbils is 20.37±0.086 g/kg.The confidence limit on 95% is(20.37±7.84) g/kg.The acute virulence is slight as relates to the graded standard of toxicant against mice.

Key words: Stellera chamaejasme, Mongolian gerbils, Botanical pesticide, Median lethal dose(LD₅₀)

摘要： 本文采用含17%瑞香狼毒(Stellera chamaejasme)根粉末的毒饵,依据孙氏改良寇氏综合计算法,实验室内测定了其对长爪沙鼠(Meriones unguiculatus)的急性毒力。根据预试验结果,采用最低剂量为7.0 g·kg^-1,最适剂量梯度比值K=0.65的5个剂量梯度的瑞香狼毒根毒饵饲喂长爪沙鼠。结果显示:试验组试鼠体重明显下降(P=0.029<0.05),完全致死组肝脏重/体重比对照组明显减小(P=0.014<0.05),但肾脏略增大(P=0.102>0.05)。其对长爪沙鼠的LD50为(20.37±0.086)g·kg^-1,95%平均可信限为(20.37±7.84)g·kg^-1,属弱毒水平。

关键词: 瑞香狼毒, 长爪沙鼠, 生物农药, 致死中量

CLC Number:

Q95-33
S326

SHI Kun, ZHOU Da-sen, GUO Yong-wang, SHI Da-zhao, WANG Deng. Acute Toxicity Analyses of Stellera chamaejasme Root in Mongolian Gerbils(Meriones unguiculatus)[J]. , 2010, 18(5): 731-734.

史琨, 周大森, 郭永旺, 施大钊, 王登. 瑞香狼毒根对长爪沙鼠的急性毒力测试[J]. , 2010, 18(5): 731-734.

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Acute Toxicity Analyses of Stellera chamaejasme Root in Mongolian Gerbils(Meriones unguiculatus)

瑞香狼毒根对长爪沙鼠的急性毒力测试

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