›› 2011, Vol. 19 ›› Issue (4): 689-693.DOI: 10.11733/j.issn.1007-0435.2011.04.026

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Effect of Chinese Herbal Medicine Additives on Ram Semen Quality and Freezing Tolerance

QIU Dai-yu1, ZHANG Zhao-wang1, LIN Hai-ming1, YANG Bao-tian1, LI Ying-dong2   

  1. 1. Agronomy College of Gansu Agricultural University, Lanzhou, Gansu Province 730070, China;
    2. Gansu College of Traditional Chinese Medicine, Lanzhou, Gansu Province 730050, China
  • Received:2010-10-18 Revised:2011-05-04 Online:2011-08-15 Published:2011-08-15

中草药添加剂对种公羊精液品质和耐冻性的影响

邱黛玉1, 张兆旺1, 蔺海明1, 杨保田1, 李应东2   

  1. 1. 甘肃农业大学, 兰州730070;
    2. 甘肃中医学院, 兰州730050
  • 通讯作者: 张兆旺,E-mail:Zhangzhaowang@gsau.edu.Cn
  • 作者简介:邱黛玉(1978- ),女,甘肃民勤人,博士,讲师,主要从事药用植物资源与利用研究,E-mail:qiudy@gsau.edu.cn
  • 基金资助:
    农业部“948”项目(2009-Z19);甘肃省农牧厅中药材攻关项目(gyc09-06);国家科技支撑计划项目(2007BAI37B00)资助

Abstract: To explore the effect of Chinese herbal medicine on ram semen quality,19 kinds of Chinese herbal medicines as feed additives (adding 1% daily feed quantity per ram) were fed to rams for 3 weeks.The sexual reaction and semen quality of rams were studied after feeding Chinese herbal medicine additives.Results showed that sexual reaction time was shortened significantly compared with contro.Ejaculate volume and sperm density were increased significantly in test groups compared with that in the control group.Effective sperm number was up to 61.64 × 109 every time,which was significantly higher than that in control group by about 2.36 times (P<0.01).Sperm head deformity was about 8% lower than that of control.Semen quality had significantly improved after feeding with Chinese herbal medicine feed additives for one week.The rate of sperm quality decline was delayed until one week after three weeks feeding.After freezing,both sperm motility rate and recovery rate were significantly higher than that of control (P<0.05).Sperm motility rate of tested group increased 38.7% and sperm recovery rate was increased 28.9% over the control after freezing (P<0.05).Therefore,Chinese herbal medicine as feed additives can be used to improve the quality of ram’s semen,and to improve the storage quality of semen in artificial insemination process.

Key words: Ram, Chinese herbal medicine additives, Semen quality, Freezing tolerance

摘要: 为探索中草药添加剂对种公羊精液品质的影响,用当归(Radix angelicae sinensis)、党参(Radix codonop sis)、黄芪(Radix astrag ali)、淫羊藿(Herba epimedii)、菟丝子(Semen cuscutae)等19味中草药复方配伍作饲料添加剂,按每头种公羊饲料量1%的比例饲喂3周,对种公羊的性反应和精液品质进行分析研究。结果表明:饲喂中草药添加剂后公羊性反应时间显著缩短;射精量、精子密度较对照显著和极显著提高;每次射精有效精子数达到61.64×109,比对照组增加2.36倍,差异极显著(P<0.01);精子头部畸形率较对照组降低8%(P<0.05);饲喂1周对精液质量有明显改善,饲喂3周至停药1周后可延缓精子质量下降的速度;且饲喂后种公羊的冻后精子活率和精子复苏率较对照和饲喂前显著提高(P<0.05),分别增加38.7%和28.9%。该复方中草药添加剂适用于高频度采精种公羊,可改善公羊的精液品质,提高人工授精过程中精液保存质量,有利于进一步发挥优良公羊的种用价值。

关键词: 种公羊, 中草药添加剂, 精液品质, 耐冻性

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