Isolation and Identification of Hemerocallin-Producing Fungal Endophyte

doi:10.11733/j.issn.1007-0435.2012.05.022

›› 2012, Vol. 20 ›› Issue (5): 935-939.DOI: 10.11733/j.issn.1007-0435.2012.05.022

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Isolation and Identification of Hemerocallin-Producing Fungal Endophyte

LIANG Jie¹, YANG Kai¹, LI Qin-fan¹, JIANG Zhen-guo^1,2, KONG Xiang-ya¹, YANG Guo-dong¹, JIN Yi-min¹

1. Collage of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province 712100, China;
2. Chang'an Centre for Animal Disease Control & Prevention, Chang'an, Shaanxi Province 710100

Received:2012-03-20 Revised:2012-05-18 Online:2012-10-15 Published:2012-11-01

产萱草根素内生真菌的分离与鉴定

梁洁¹, 杨凯¹, 李勤凡¹, 姜振国^1,2, 孔祥雅¹, 杨国栋¹, 金意敏¹

1. 西北农林科技大学动物医学院, 陕西杨凌 712100;
2. 西安市长安区动物疾病预防控制中心, 陕西长安 710100

通讯作者: 李勤凡,E-mail:liqinfan@yahoo.com.cn
作者简介:梁洁(1987-),女,陕西西安人,硕士,主要从事动物中毒性疾病的研究,E-mail:liangjie1963@163.com
基金资助:
国家自然科学基金项目(30871901);陕西省科技攻关项目(2008k02-06-2)资助

Abstract

Abstract: The study was to explore whether the hemerocallin-secret fungal endophyte exist in roots of Hemerocallis. Four strains of endophytic fungi were isolated and obtained from the H. esculenta. Four isolates were detected by thin-layer chromatography and UV spectrophotometry after culture in potato dextrose agar (PDA) for 20 days. Results showed that XC-1A, a strain of endophytic fungus producing hemerocallin was isolated and obtained from H. esculenta, and hemerocallin yield is 359.88 μg·g^-1. Morphological evidence and rDNA-ITS sequence analsis indicated that the endophyte was closely related to Zalerion varium. The hemerocallin-producing fungal endophytes exist in the root of H. esculenta.

Key words: H. esculenta, Hemerocallin, Fungal endophyte

摘要： 为探讨萱草(Hemerocallis)根中是否存在产生萱草根素的内生真菌,从北萱草(H.esculenta)根中分离得到4株内生真菌,在马铃薯(Solanum tuberosum)葡萄糖琼脂培养基(PDA)上培养20 d后收集菌丝,应用薄层色谱法和紫外分光光度法分别检测菌丝提取液中的萱草根素,筛选可产生萱草根素的内生真菌。结果表明:菌株XC-1A为产萱草根素内生真菌,含量达359.88 μg·g^-1,根据形态学观察和5.8S rDNA-ITS序列分析结果,确定XC-1A为Zalerion varium。北萱草中存在可产生萱草根素的内生真菌。

关键词: 北萱草, 萱草根素, 内生真菌

CLC Number:

LIANG Jie, YANG Kai, LI Qin-fan, JIANG Zhen-guo, KONG Xiang-ya, YANG Guo-dong, JIN Yi-min. Isolation and Identification of Hemerocallin-Producing Fungal Endophyte[J]. , 2012, 20(5): 935-939.

梁洁, 杨凯, 李勤凡, 姜振国, 孔祥雅, 杨国栋, 金意敏. 产萱草根素内生真菌的分离与鉴定[J]. , 2012, 20(5): 935-939.

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Isolation and Identification of Hemerocallin-Producing Fungal Endophyte

产萱草根素内生真菌的分离与鉴定

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