Effects of Cold Stress on the Growths and Physiological Characteristics of Two Elymus nutans Varieties

doi:10.11733/j.issn.1007-0435.2014.04.017

Acta Agrestia Sinica ›› 2014, Vol. 22 ›› Issue (4): 789-795.DOI: 10.11733/j.issn.1007-0435.2014.04.017

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Effects of Cold Stress on the Growths and Physiological Characteristics of Two Elymus nutans Varieties

FU Juan-juan¹, LIU Jian¹, SUN Yong-fang¹, CHU Xi-tong¹, MIAO Yan-jun², HU Tian-ming¹, XU Yue-fei¹

1. Department of Grassland Science, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi Province 712100, China;
2. College of Plant Science, Tibet Agriculture and Animal Husbandry College, Linzhi, Tibet 860000, China

Received:2013-10-21 Revised:2013-12-16 Online:2014-08-15 Published:2014-08-04

冷胁迫对2种垂穗披碱草生长和生理特性的影响

付娟娟¹, 刘建¹, 孙永芳¹, 褚希彤¹, 苗彦军², 呼天明¹, 许岳飞¹

1. 西北农林科技大学动物科技学院, 陕西杨凌 712100;
2. 西藏农牧学院植物科学学院, 西藏林芝 860000

通讯作者: 呼天明, 许岳飞
作者简介:付娟娟（1987-），女，蒙古族，内蒙古赤峰人，硕士研究生，研究方向为植物逆境生理生态，E-mail：fujuanjuan1234@126.com
基金资助:
西北农林科技大学基本科研业务费专项（QN2011100）；国家科技支撑计划（2011BAD17B05）（2011BAC09B03）资助

Abstract

Abstract:

Low temperature is one of the limiting factors affecting plant growth. The growths and physiological characteristics of Tibetan wildElymus nutans and Gannan E. nutans were studied under cold stress with sand culture in this paper. The results showed that cold stress significantly inhibited the seedling growths and chlorophyll syntheses of two E. nutans varieties. Gannan E. nutans leaves were more liable to suffer oxidative damage than the wild E. nutans leaves. The electrolyte leakages and MDA contents of tested varieties increased by 114.7%, 124.6% and 18.2%, 10.8% comparing to control, respectively. During the period of cold stress, the accumulations of hydrogen peroxide (H₂O₂) and superoxide radical in E. nutans increased compared with the control. Damxung E. nutans maintained significantly higher carotenoids (Car) content and the activities of antioxidant enzyme, superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) were 80.3%, 27.3% and 65.9% higher than the control. Correlation analyses indicated there were significant correlations between growth and physiology charaterisitics, as well as lipid peroxidation and antioxidant enzyme activities in E. nutans leaves.It can be concluded that Dangxiong specices exhibits a better tolerance to cold stress than Gannan.

Key words: Cold stress, Lipid peroxidation, ROS accumulation, Antioxidant enzyme, Elymus nutans

摘要：

低温是影响植物生长的外界限制因子之一。采用沙培法研究了冷胁迫对2种垂穗披碱草（Elymus nutans）（当雄垂穗披碱草（西藏野生）和甘南垂穗披碱草）生长与生理特性的影响。结果表明：冷胁迫处理5d显著抑制2种垂穗披碱草的生长和叶绿素的合成，特别是甘南垂穗披碱草发生严重氧化损伤，电解质渗透率和丙二醛（MDA）含量分别比对照增加114.7%和124.6%，而当雄垂穗披碱草受伤害较轻，分别比对照增加18.2%和10.8%。冷胁迫也显著增加了甘南垂穗披碱草中H₂O₂和自由基的积累，而当雄垂穗披碱草与对照相比差异不显著。冷胁迫处理5d后，当雄垂穗披碱草的类胡萝卜素含量较甘南增加72.3%，超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和过氧化物酶（POD）的活性分别较对照增加80.3%，27.3%和65.9%；甘南垂穗披碱草的SOD和POD活性分别较对照增加11.4%和28.9%，而其CAT活性下降25.5%。相关分析表明，垂穗披碱草在冷胁迫下细胞膜受损伤程度与抗氧化酶活性负相关。当雄垂穗披碱草通过提高抗氧化酶活性，可有效清除体内积累的活性氧，减轻细胞膜系统受损伤的程度从而提高其抗寒性。

关键词: 冷胁迫, 膜脂过氧化, 活性氧, 抗氧化酶, 垂穗披碱草

CLC Number:

FU Juan-juan, LIU Jian, SUN Yong-fang, CHU Xi-tong, MIAO Yan-jun, HU Tian-ming, XU Yue-fei. Effects of Cold Stress on the Growths and Physiological Characteristics of Two Elymus nutans Varieties[J]. Acta Agrestia Sinica, 2014, 22(4): 789-795.

付娟娟, 刘建, 孙永芳, 褚希彤, 苗彦军, 呼天明, 许岳飞. 冷胁迫对2种垂穗披碱草生长和生理特性的影响[J]. 草地学报, 2014, 22(4): 789-795.

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References

[1] 董玉琛,刘旭. 中国作物及其野生近缘植物[M]. 北京:中国农业出版社,2007:142-144
[2] 陈默君,贾慎修.中国饲用植物[M]. 北京:中国农业出版社,2002:119-120
[3] 吴雪霞,查丁石,邰翔. 低温胁迫对茄子幼苗生长、抗氧化酶活性和渗透调节物质的影响[J]. 江苏农业学报,2008,24(4):471-475
[4] Sekmen A H, Turkan I, Tanyolac Z O, et al. Different antioxidant defense responses to salt stress during germination and vegetative stages of endemic halophyte Gypsophila oblanceolata Bark [J]. Environmental and Experimental Botany,2012,77:63-76
[5] 任华中,黄伟,张福墁. 低温弱光对番茄幼苗光合特性的影响[J]. 中国农业大学学报,2002,7(1):95-101
[6] 乌凤章,王柏臣,刘桂丰,等. 低温胁迫对白桦幼苗生长与生理的影响[J]. 东北林业大学学报,2008,36(9):8-10
[7] 严青,马玉寿,施建军,等. 低温胁迫对3种牧草幼苗抗性生理指标的影响[J]. 青海大学学报:自然科学版,2007,25(1):54-57
[8] Aroca R, Irigoyen J J, Sánchez-Díaz M. Photosynthetic characteristics and protective mechanisms against oxidative stress during chilling and subsequent recovery in two maize varieties differing in chilling sensitivity [J]. Plant Science,2001,161(4):719-726
[9] Korkmaz A, Korkmaz Y, Demirkíran A R. Enhancing chilling stress tolerance of pepper seedlings by exogenous application of 5-aminolevulinic acid [J]. Environmental and Experimental Botany,2010,67(3):495-501
[10] 许大全. 叶绿素含量测定及其应用中的几个问题[J].植物生理学通讯,2009,45(9):896-898
[11] Song L L, Ding W, Zhao M G, et al. Nitric oxide protects against oxidative stress under heat stress in the calluses from two ecotypes of reed [J]. Plant Science,2006,171(4):449-458
[12] Li Y, Zhao H, Duan B, et al. Effect of drought and ABA on growth, photosynthesis and antioxidant system of Cotinus coggygriaseedlings under two different light conditions [J]. Environmental and Experimental Botany,2011,71(1):107-113
[13] Veljovic-Jovanovic S, Noctor G, Foyer C H. Are leaf hydrogen peroxide concentrations commonly overestimated? The potential influence of artefactual interference by tissue phenolics and ascorbate [J]. Plant Physiology and Biochemistry,2002,40(6/8):501-507
[14] Elstner E F, Heupel A. Inhibition of nitrite formation from hydroxylammo-niumchloride: A simple assay for superoxide dismutase [J]. Analytical Biochemistry,1976,70(2):616-620
[15] Beauchamp C, Fridovich I. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels [J]. Analytical Biochemistry,1971,44(1):276-287
[16] Upadhyaya A, Sankhla D, Davis T D, et al. Effect of paclobutrazol on the activities of some enzymes of activated oxygen metabolism and lipid peroxidation in senescing soybean leaves [J]. Journal of Plant Physiology,1985,121(5):433-461
[17] Cakmak I, Marschner H. Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bean leaves [J].Journal of Plant Physiology,1992,98(4):1222-1227
[18] Bradford M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding [J]. Analytical Biochemistry,1976,72(1/2):248-254
[19] 许岳飞,金晶炜,孙小玲,等. 一氧化氮对强光胁迫下高羊茅抗氧化系统的调节效应[J].生态学杂志,2009,28(12):2471-2476
[20] Bertamini M, Muthuchelian K, Rubinigg M, et al. Photoinhibition of photosynthesis in leaves of grapevine (Vitis viniferaL.cv.Riesling). Effect of chilling nights [J]. Photosynthetica,2005,43(4):551-557
[21] 许楠,孙广玉. 低温锻炼后桑树幼苗光合作用和抗氧化酶对冷胁迫的响应[J]. 应用生态学报,2009,20(4):761-766
[22] 叶晓青,王松凤,汤日圣,等. 低温处理对红粉佳人生理生化特性的影响[J]. 江苏农业学报,2008,24(5):612-615
[23] 蔡仕珍,潘远智,陈其兵,等. 低温胁迫对花叶细辛生理生化和生长的影响[J]. 草业学报,2010,19(1):95-102
[24] Balestrasse K B, Tomaro M L, Batlle A, et al. The role of 5-aminolevulinic acid in the response to cold stress in soybean plants [J]. Phytochemistry,2010,71(17/18):2038-2045
[25] Xie Z X, Duan L S, Tian X L, et al. Coronatine alleviates salinity stress in cotton by improving the antioxidative defense system and radical-scavenging activity [J]. Journal of Plant Physiology,2008,165(4):375-384
[26] 刘慧英,朱祝军,吕国华.低温胁迫对嫁接西瓜耐冷性和活性氧清除系统的影响[J]. 应用生态学报,2004,15(4):659-662
[27] Mittler R, Vanderauwera S, Gollery M, et al. Reactive oxygen gene network of plants [J]. Trends in Plant Science,2004,9(10):490-498

Effects of Cold Stress on the Growths and Physiological Characteristics of Two Elymus nutans Varieties

冷胁迫对2种垂穗披碱草生长和生理特性的影响

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