Effects of Different Concentrations of Hormones on the Regeneration System of Tall Fescue

doi:10.11733/j.issn.1007-0435.2015.03.013

Acta Agrestia Sinica ›› 2015, Vol. 23 ›› Issue (3): 517-525.DOI: 10.11733/j.issn.1007-0435.2015.03.013

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Effects of Different Concentrations of Hormones on the Regeneration System of Tall Fescue

GUO Ling-na¹, LIU Jian-wen², MA Dong-mei², XU Xing²

1. Ningxia University, School of life science, Yinchuan, Ningxia 750021, China;
2. Ningxia University, School of agriculture, Yinchuan, Ningxia 750021, China

Received:2014-05-20 Revised:2014-08-31 Online:2015-06-15 Published:2015-07-02

不同浓度激素配比对高羊茅再生体系建立的影响

郭伶娜¹, 刘建文², 麻冬梅², 许兴²

1. 宁夏大学生命科学学院, 宁夏银川 750021;
2. 宁夏大学农学院, 宁夏银川 750021

通讯作者: 许兴
作者简介:郭伶娜(1989-),女,宁夏中卫人,硕士研究生,研究方向为植物基因工程,E-mail:guolingna2008@163.com
基金资助:
国家“973” 项目(2012CB114204);自治区牧草育种专项(NYYZ-2014-Z12)资助

Abstract

Abstract:

The impacts of manifold factors on callus induction from mature seeds, subculture production and embryogenic calli differentiation were studied here. Plant regeneration system of tall fescue (Festuca arundinacea Schreb.) embryogenic calli was established using the sterile seeds of Arid 3 variety as explants. The results showed that the best callus induction medium was MS+5.5 mg·L^-1 2,4-D+500 mg·L^-1 CH; and MS+2.0 mg·L^-1 6-BA+1.0 mg·L^-1 KT medium performed the best function in the differentiation of tall fescue embryogenic calli. The best rooting medium was 1/2MS+0.3 mg·L^-1IBA, and the content of sucrose was 20 g·L^-1.

Key words: Turfgrass, Tissue culture, Regeneration system

摘要：

以‘爱瑞3号’为试材,以无菌种子为外植体,对成熟种子愈伤组织诱导、胚性愈伤组织继代和分化的影响因素进行了研究,构建了高频的高羊茅胚性愈伤组织植株再生体系。结果表明:最佳愈伤诱导培养基配方为MS+5.5 mg·L^-1 2,4-D+500 mg·L^-1 CH;最佳分化培养基配方为MS+2.0 mg·L^-16-BA+1.0 mg·L^-1 KT;最佳生根培养基配方为1/2MS+0.3 mg·L^-1 IBA, 其蔗糖含量为20 g·L^-1。

关键词: 草坪草, 组织培养, 再生体系

CLC Number:

S543.9

GUO Ling-na, LIU Jian-wen, MA Dong-mei, XU Xing. Effects of Different Concentrations of Hormones on the Regeneration System of Tall Fescue[J]. Acta Agrestia Sinica, 2015, 23(3): 517-525.

郭伶娜, 刘建文, 麻冬梅, 许兴. 不同浓度激素配比对高羊茅再生体系建立的影响[J]. 草地学报, 2015, 23(3): 517-525.

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Effects of Different Concentrations of Hormones on the Regeneration System of Tall Fescue

不同浓度激素配比对高羊茅再生体系建立的影响

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