Impact of Cytokinin on the Antioxidative Physiology of Creeping Bentgrass under Drought Stress

doi:10.11733/j.issn.1007-0435.2015.06.011

Acta Agrestia Sinica ›› 2015, Vol. 23 ›› Issue (6): 1206-1213.DOI: 10.11733/j.issn.1007-0435.2015.06.011

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Impact of Cytokinin on the Antioxidative Physiology of Creeping Bentgrass under Drought Stress

LIU Yang¹, LI Rong-quan², ZHANG Xun-zhong³, CHANG Zhi-hui¹

1. Turf Institute of Beijing Forestry University, Beijing 100083, China;
2. Shenyang Lanxi Green Technology Development Co., Ltd., Shenyang, Liaoning Province 110000, China;
3. Department of Crop and Soil Environmental Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA

Received:2014-05-13 Revised:2014-07-22 Online:2015-12-15 Published:2016-02-01

干旱胁迫下细胞分裂素对匍匐翦股颖抗氧化生理的影响

刘洋¹, 李荣全², 张训忠³, 常智慧¹

1. 北京林业大学草坪研究所, 北京 100083;
2. 沈阳兰溪绿化技术开发有限责任公司, 辽宁沈阳 110000;
3. 美国弗吉尼亚理工大学作物与土壤环境科学系, 弗吉尼亚州 24061

通讯作者: 常智慧
作者简介:刘洋(1988-),男,河北邢台人,硕士,主要从事草坪建植与管理研究,E-mail:gnliuyang@163.com
基金资助:
公益性行业科研专项经费项目(201310289-2);奥林高尔夫教育与研究专项基金(AL2013007)资助

Abstract

Abstract:

In this experiment, the effect of cytokinin on Creeping bentgrass(Agrostis stolonifera) oxidize-resistant physiology by spraying different concentrations of exogenous cytokinin was studied. Treatments included two moisture conditions: well-watered and drought stress; and 3 concentrations of cytokinin: 0, 10 and 100 μM. Leaf relative water contents, levels of reactive oxygen species (O₂^- and H₂O₂), superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) and ascorbate perxidase (APX) activities, and malondialdehyde (MDA) contents were determined. The results indicated that cytokinin reduced the generation rate of O₂^- and H₂O₂, MDA contents of creeping bentgrass compared to control under both water regimes (P <0.05); and increased the antioxidant enzymes activities and leaf relative water contents of the bentgrass under drought stress (P <0.05). These results indicated that cytokinin improved the drought tolerance of creeping bentgrass. In the late stages of drought stress, the indicators of creeping bentgrass with high concentration (100 μM) cytokinin treatment showed significant advantages (P <0.05). These results indicated that the high concentration of cytokinin had a better promotion to creeping bentgrass under drought stress.

Key words: Cytokinin, Drought, Creeping bentgrass, Antioxidative physiology

摘要：

为探索干旱胁迫下细胞分裂素对匍匐翦股颖(Agrostis stolonifera)活性氧水平变化及生理应答的影响,试验设计2种水分条件:干旱胁迫和充分浇水;3种浓度细胞分裂素类物质(TZR)处理:0,10,100 μM。研究匍匐翦股颖叶片相对含水量、活性氧水平(O₂^-和H₂O₂)以及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)活性和丙二醛(MDA)含量的变化。结果表明:与对照相比,在2种水分条件下,2种浓度的TZR均能显著降低匍匐翦股颖的O₂^-产生速率、H₂O₂含量和MDA含量(P <0.05),显著提高4种抗氧化酶的活性(P <0.05);同时干旱条件下,TZR对提高匍匐翦股颖叶片相对含水量效果明显,表明TZR能够增强匍匐翦股颖的耐旱性;在干旱胁迫后期,高浓度(100 μM)TZR处理的匍匐翦股颖各项指标均表现出显著的优势(P <0.05),表明高浓度TZR对匍匐翦股颖抵御干旱胁迫的作用更大。

关键词: 细胞分裂素, 干旱, 匍匐翦股颖, 抗氧化生理

CLC Number:

LIU Yang, LI Rong-quan, ZHANG Xun-zhong, CHANG Zhi-hui. Impact of Cytokinin on the Antioxidative Physiology of Creeping Bentgrass under Drought Stress[J]. Acta Agrestia Sinica, 2015, 23(6): 1206-1213.

刘洋, 李荣全, 张训忠, 常智慧. 干旱胁迫下细胞分裂素对匍匐翦股颖抗氧化生理的影响[J]. 草地学报, 2015, 23(6): 1206-1213.

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Impact of Cytokinin on the Antioxidative Physiology of Creeping Bentgrass under Drought Stress

干旱胁迫下细胞分裂素对匍匐翦股颖抗氧化生理的影响

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