Acta Agrestia Sinica

Acta Agrestia Sinica ›› 2016, Vol. 24 ›› Issue (1): 93-100.DOI: 10.11733/j.issn.1007-0435.2016.01.013

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Responses of Growth and Physiology Metabolism of Ophiopogon japonicus to NaCl Stress

LIU Ai-rong1, ZHANG Yuan-bing2, ZHANG Xue-ping1, YE Mei-rong1, ZHAN Qiu-wen3   

  1. 1. Life Science College, Anhui Science and Technology University, Fengyang, Anhui Province 233100, China;
    2. Urban Construction and Environment College, Anhui Science and Technology University, Fengyang, Anhui Province 233100, China;
    3. College of Agricultural, Anhui Science and Technology University, Fengyang, Anhui Province 233100, China
  • Received:2015-01-12 Revised:2015-06-23 Online:2016-02-15 Published:2016-04-26

麦冬生长及相关生理代谢对NaCl胁迫的响应

刘爱荣1, 张远兵2, 张雪平1, 叶梅荣1, 詹秋文3   

  1. 1. 安徽科技学院生命科学院, 安徽 凤阳 233100;
    2. 安徽科技学院城建与环境学院, 安徽 凤阳 233100;
    3. 安徽科技学院农学院, 安徽 凤阳 233100
  • 作者简介:刘爱荣(1966-),女,安徽怀宁人,教授,主要从事植物环境生理方面研究,E-mail:liuar@ahstu.edu.cn
  • 基金资助:

    安徽省科技厅农业科技成果转化资金项目(1404032007);安徽省科技厅攻关计划项目(1301031030);安徽省科技厅2007年度重点科研项目(07020304093);安徽省省级学科建设重大项目-草学(皖教秘科[2014]28号);安徽科技学院重点学科风景园林学(AKZDXK2015B01)资助

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Abstract:

The responses of growth and physiology metabolisms of O. japonicus to NaCl stress were studied using potted plants treated with the NaCl solutions of different concentrations (0, 50, 100, 150, and 200 mmol·L-1). As NaCl concentration increased, the fresh and dry weight, water content, nitrate reductase activity, the contents of K+, Ca2+, Mg2+, soluble protein of root and leaf, soluble sugar content of leaf, and root activity decreased compared with the control, whereas membrane permeability, the contents of NO3-1, Na+, MDA, free amino acids, proline of root and leaf, and soluble sugar content of root increased. And root/shoot ratio initially showed a slightly increased tendency then decreased. Therefore, the decreases of different degrees of the water deficit, NO3-1 reduction ability, main species and accumulation amounts of osmotica, and membrane stability of O. japonicus root and leaf under NaCl stress caused the different sensitivities of root and leaf growth to NaCl stress. The inhibition effect of NaCl stress on root growth was smaller than on leaves under the NaCl stress of 50~150 mmol·L-1, while that was greater than the leaves under the NaCl stress of 150~200 mmol·L-1. Comprehensive analysis showed that O. japonicus could tolerate the NaCl stress of 150 mmol·L-1, and the damage would increase and aggravate with the increase of the NaCl concentration.

Key words: Ophiopogon japonicus, NaCl stress, Growth, Root/shoot ratio, Osmotica, Plasma membrane stability

摘要：

以不同浓度的NaCl(0,50,100,150和200mmol·L-1)处理盆栽麦冬,研究麦冬根、叶的生长和相关生理代谢对NaCl胁迫的响应。结果表明:与对照相比,随着NaCl浓度增加,麦冬根和叶的鲜重、干重、含水量、硝酸还原酶(nitrate reductase,NR)活性、K+,Ca2+,Mg2+、可溶性蛋白、叶的可溶性糖含量及根系活力均呈下降趋势;根冠比呈先略上升后下降趋势;根和叶的质膜透性、NO3-、Na+、游离氨基酸、脯氨酸(proline,Pro)、丙二醛(malonaldehyde,MDA)以及根的可溶性糖含量均呈上升趋势。因此,盐胁迫下麦冬根和叶的水分亏缺程度、还原NO-3能力、主要渗透调节物质的种类和积累量、以及质膜稳定性下降程度均不尽相同,使其根和叶生长对NaCl胁迫敏感程度也不同;以50~100mmol·L-1的NaCl胁迫对根生长的抑制作用小于叶,而150~200mmol·L-1的NaCl胁迫对根生长的抑制作用大于叶。综合分析显示,麦冬能耐150mmol·L-1的NaCl胁迫,大于此浓度,盐伤害加重。

关键词: 麦冬, NaCl胁迫, 生长, 根冠比, 渗透调节物质, 质膜稳定性

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