Effects of Grazing Intensity on the Bacterial Number and Community Characteristics in Bulk and Rhizosphere Soil of Stipagrandis

doi:10.11733/j.issn.1007-0435.2016.05.006

Acta Agrestia Sinica ›› 2016, Vol. 24 ›› Issue (5): 967-974.DOI: 10.11733/j.issn.1007-0435.2016.05.006

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Effects of Grazing Intensity on the Bacterial Number and Community Characteristics in Bulk and Rhizosphere Soil of Stipagrandis

LI Ting-ting¹, HAI Li-li¹, HAN Bing¹, ZHAO Meng-li², ZHAO Hong-bin¹

1. The Functional Laboratory of Plant Biotechnology, College of Life Science, Inner Mongolia Agricultural University, Huhhot, Inner Mongolia 010018, China;
2. College of Ecology and Environmental Science, Inner Mongolia Agricultural University, Huhhot, Inner Mongolia 010018, China

Received:2015-11-18 Revised:2016-03-15 Online:2016-10-15 Published:2017-01-13

放牧强度对大针茅根际和非根际土壤中细菌数量和群落特征的影响

李婷婷¹, 海丽丽¹, 韩冰¹, 赵萌莉², 赵鸿彬¹

1. 内蒙古农业大学生命科学学院植物生物技术功能实验室, 内蒙古呼和浩特 010018;
2. 内蒙古农业大学生态环境学院, 内蒙古呼和浩特 010018

通讯作者: 韩冰
作者简介:李婷婷(1986-),女,河南郑州人,硕士研究生,主要从事分子生物学研究,E-mail:ting_cn@tom.com
基金资助:
国家自然科学基金（31360113）；内蒙古自然科学基金（2015MS0305）资助

Abstract

Abstract:

In order to explore the effects of grazing intensity on the bacterial number and community characteristics in bulk and rhizosphere soil of Stipagrandis, we investigated the sizes and community structures of bacteria in the rhizosphere and bulk soils of Stipagrandis under four grazing intensities using spread plate method and denatured gradient gel electrophoresis (DGGE). Four grazing intensities were non-grazing (CK), lightgrazing(LG), moderate grazing(MG) and heavy grazing(HG), respectively. The results showed that grazing intensity had significant effect on bacterial community diversity and structure, but bacterial number was not significantly impacted by grazing intensity. Bacterial number and community characteristic in rhizosphere soil or bulk soil showed different patterns along with grazing intensity. In rhizosphere soil, bacterial number was the highest in MG plot and bacterial community diversity was the highest in LG plot. However, in the bulk soil, bacterial number and community diversity gradually decreased with increasing the grazing intensity. This meaned rhizosphere bacteria were more resistant against grazing than non-rhizosphere bacteria. Rhizosphere bacteria might be a pool of bacteria in the process of ecological restoration.

Key words: Grazing intensity, Rhizosphere, Bulk soil, Stipagrandis, DGGE, Bacterial community

摘要：

利用涂布平板法和变性梯度凝胶电泳（denatured gradient gel electrophoresis，DGGE）技术，检测4个放牧强度（对照、轻度放牧、中度放牧和重度放牧）下，大针茅（Stipagrandis）植物根际和非根际土壤中细菌数量和群落特征的变化。结果表明，放牧对细菌群落多样性及组成有显著影响，但对细菌数量没有明显影响。随着放牧强度的增加，根际和非根际土壤细菌数量及群落多样性的变化趋势不同。根际土壤中，在中度放牧强度下，细菌数量最高，轻度放牧强度下，细菌多样性最高。说明细菌群落多样性比细菌数量敏感。而在非根际土壤，细菌数量和群落多样性随着放牧强度的增加而逐渐降低。说明根际细菌较非根际细菌群落有更好的抗干扰能力。同一放牧强度下，根际土壤细菌数量及群落多样性高于非根际土壤，但根际和非根际土壤细菌群落结构差异较小。总之，与非根际细菌相比，根际细菌群落具有更好的抗干扰能力，及其与非根际土壤细菌群落组成的相似性说明在生态恢复过程中，根际细菌群落可能作为一个菌种库存在。

关键词: 放牧强度, 根际, 非根际, 大针茅, DGGE, 细菌群落

CLC Number:

S154.381

LI Ting-ting, HAI Li-li, HAN Bing, ZHAO Meng-li, ZHAO Hong-bin. Effects of Grazing Intensity on the Bacterial Number and Community Characteristics in Bulk and Rhizosphere Soil of Stipagrandis[J]. Acta Agrestia Sinica, 2016, 24(5): 967-974.

李婷婷, 海丽丽, 韩冰, 赵萌莉, 赵鸿彬. 放牧强度对大针茅根际和非根际土壤中细菌数量和群落特征的影响[J]. 草地学报, 2016, 24(5): 967-974.

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References

[1] Dey R, Pal K K, Tilak K V B R. Influence of soil and plant types on diversity of rhizobacteria[J]. Proceedings of the National Academy of Sciences India Section B:Biological Science,2012,82(3):341-352
[2] Abawi G S, Widmer T L. Impact of soil health management practices on soilborne pathogens and root diseases of vegetables[J]. Applied Soil Ecology,2000,15(1):37-47
[3] García-Salamanca A, Molina-Henares M A, van Dillewijn P, et al. Bacterial diversity in the rhizosphere of maize and the surrounding carbonate-rich bulk soil[J]. Microbial Biotechnology,2013,6(1):36-44
[4] Kowalchuk G A, Buma D S, de Boer W, et al. Effects of above-ground plant species composition and diversity on the diversity of soil-borne microorganisms[J]. Antonie Van Leeuwenhoek,2002,81(1):509-520
[5] Xiaoqi Zhou, Jinzhi Wang, Yanbin Hao, et al. Intermediate grazing intensities by sheep increase soil bacterial diversities in an Inner Mongolian steppe[J]. Biology and Fertility of Soils,2010,46(8):817-824
[6] K Klumpp, S Fontaine, E Attard, et al. Grazing triggers soil carbon lossby altering plant roots and their control on soil microbial community[J]. Journal of Eology,2009,97(5):876-885
[7] 张永生,赖欣,张静妮,等. 放牧对贝加尔针茅草原土壤细菌群落结构的影响[J]. 生态学杂志,2010,29(12):2457-2463
[8] Sørensen J. The rhizosphere as a habitat for soil microorganisms[C]//Elsas J D van, Trevors J T, Wellington E M H. Modern soil microbiology.New York:Marcel Dekker,Inc.,1997:21-45
[9] Jin J, Wang G H, Liu X B, et al. Temporal and spatial dynamics of bacterial community inthe rhizosphere of soybean genotypes grown in a blacksoil[J]. Pedosphere, 2009,19(6):808-816
[10] Lambers H, Mougel C, Jaillard B. Plant-microbe-soil interactions in the rhizosphere:an evolutionaryperspective[J]. Plant and Soil,2009,321(1-2):83-115
[11] 王纳纳,陈颖,应娇妍,等. 内蒙古草原典型植物对土壤微生物群落的影响[J]. 植物生态学报,2014,38(2):201-208
[12] 胡静,侯向阳,王珍,等. 割草和放牧对大针茅根际与非根际土壤养分和微生物数量的影响[J]. 应用生态学报,2015,26(11):3482-3488
[13] 中国科学院内蒙古宁夏综合考察队. 内蒙古植被[M]. 北京:科学出版社,1985:495-515
[14] 珊丹,赵萌莉,韩冰,等. 不同放牧压力下大针茅种群的遗传多样性[J]. 生态学报,2006,26(10):3175-3183
[15] 李永宏.内蒙古锡林河流域羊草草原和大针茅草原在放牧影响下的分异和趋同[J]. 植物生态学与地植物学报,1988,12(3):189-196
[16] 李博. 中国北方草地退化及其防治对策[J]. 中国农业科学,1997,30(6):1-9
[17] 王仁忠,李建东. 采用系统聚类分析法对羊草草地放牧演替阶段的划分[J]. 生态学报,1991,11(4):367-371
[18] Smalla K, Wieland G, Buchner A, et al. Bulk and rhizosphere soil bacterial communities studied by denaturing gradient gel electrophoresis:plant-dependent enrichment and seasonal shifts revealed[J]. Applied and Environmental Microbiology,2001,67(10):4742-4751
[19] Yang A, Liu N, Tian Q, et al. Rhizosphere bacterial communities of dominant steppe plants shift in response to a gradient of simulated nitrogen deposition[J]. Frontiers in Microbiology,2015,6:789
[20] 许光辉,郑洪元. 土壤微生物分析手册[M]. 北京:农业出版社,1986:287-289
[21] Cullen D W, Hirsch P R. Simple and rapid method for direct extraction of microbial DNA from soil for PCR[J]. Soil Biology and Biochemistry,1998,30(8/9):983-993
[22] James D M Speed, Gunnar A, Atle M. The response of plant diversity to grazing varies along an elevational gradient[J]. Journal of Ecology,2013,101(5):1225-1236
[23] Yan L, Zhou G, Zhang F. Effects of different grazing intensities on grassland production in China:a meta-analysis[J]. PLoS One,2013,8(12):e81466.
[24] Megan E M, Mark E R. Effects of grazing on grassland soil carbon:a global review[J]. Global Change Biology,2013,19(5):1347-1357
[25] 萨茹拉,侯向阳,陈海军,等. 放牧强度对典型草原土壤微生物特征的影响[J]. 中国草地学报,2013,35(5):86-91
[26] 李梓正,朱立博,林叶春,等. 呼伦贝尔草原不同退化梯度土壤细菌多样性季节变化[J]. 生态学报,2010,30(11):2883-2889
[27] 安渊,李博,杨持,等. 不同放牧率对大针茅种群结构的影响[J]. 植物生态学报,2002,26:163-169
[28] 胡静. 放牧对典型草原优势植物大针茅根际土壤养分和根际微生物的影响[D]. 呼和浩特:中国农业科学院草原研究所,2015:72
[29] 陆雅海,张福锁. 根际微生物研究进展[J]. 土壤,2006,38(2):113-121
[30] He N P,Zhang Y H, Yu Q, et al. Grazing intensity impacts soil carbon and nitrogen storage of continental steppe[J]. Ecosphere,2011,2(1):304-316
[31] Holt J A, Bristow K L, Mclvor J G. The effects of grazing pressure on soil animals and hydraulic properties of two soils in semi-arid tropical Queensland[J]. Australia Journal of Soil Research,1996,34(1):69-79
[32] 史印涛,关宇,张丞宇,等. 放牧强度对小叶章草甸土壤理化性状的影响[J]. 中国草地学报,2013,35(2):83-88
[33] Philipp Sch nbach, Hongwei Wan, Martin Gierus, et al. Grassland responses to grazing:effects of grazing intensity and management system in an Inner Mongolian steppe ecosystem[J]. Plant Soil,2011,340(1-2):103-115
[34] Hamilton E W, Frank D A. Can plants stimulate soil microbes and their own nutrient supply? Evidence from a grazing tolerant grass[J]. Ecology,2001,82(9):2397-2402
[35] 陈万杰,董亭,古琛,等. 不同放牧强度对大针茅根系特征的影响[J]. 中国草地学报,2015,37(4):86-91
[36] David R A, Robert R. Fitak A M V, et al. Abiotic factors shape microbial diversity in sonoran desert soils[J]. Applied and Environmental Microbiology,2012,78(21):7527-7537
[37] Duineveld B M, Kowalchuk G A, Keijzer A, et al. Analysis of bacterial communities in the rhizosphere of chrysanthemum via denaturing gradient gel electrophoresis of PCR-amplified 16S rRNA as well as DNA fragments coding for 16S rRNA[J]. Applied and Environmental Microbiology,2001,67(1):172-178
[38] Muyzer G, Waal E C, Uitterlinden A G. Profling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction amplified genes coding for 16S rRNA[J]. Applied and Environmental Microbiology,1993,59(3):695-700

Effects of Grazing Intensity on the Bacterial Number and Community Characteristics in Bulk and Rhizosphere Soil of Stipagrandis

放牧强度对大针茅根际和非根际土壤中细菌数量和群落特征的影响

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