Soil Microbial Properties in Alfalfa Field with Different Growing Years in Arid Desert Oasis

doi:10.11733/j.issn.1007-0435.2016.05.007

Acta Agrestia Sinica ›› 2016, Vol. 24 ›› Issue (5): 975-980.DOI: 10.11733/j.issn.1007-0435.2016.05.007

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Soil Microbial Properties in Alfalfa Field with Different Growing Years in Arid Desert Oasis

NAN Li-li¹, SHI Shang-li¹, YU Ji-hua²

1. College of Pratacultural Science, Gansu Agricultural University, Ministry of Education Key Laboratory of Ecosystem/Gansu Province Laboratory of Pratacultural Engineering;Sino-US Center for Grazingland Ecosystem Sustainability, Lanzhou, Gansu Province 730070, China;
2. College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China

Received:2015-11-05 Revised:2016-07-19 Online:2016-10-15 Published:2017-01-13

荒漠灌区不同种植年限苜蓿草地土壤微生物特性

南丽丽¹, 师尚礼¹, 郁继华²

1. 甘肃农业大学草业学院/草业生态系统教育部重点实验室/甘肃省草业工程实验室/中-美草地畜牧业可持续研究中心, 甘肃兰州 730070;
2. 甘肃农业大学农学院, 甘肃兰州 730070

通讯作者: 师尚礼, 郁继华
作者简介:南丽丽(1979-),女,甘肃天水人,博士,副教授,主要从事牧草栽培与育种研究,E-mail:nanll@gsau.edu.cn
基金资助:
农业部行业专项子课题（201403048-8）；甘肃省高校基本科研业务费；国家自然基金（31460630，41163002）专项资助

Abstract

Abstract:

Using fumigation extraction method and microbial culture method, this paper investigated the microbial biomass and soil microbial quantities of alfalfa field in 0~60 cm soil layer with different growing years (0,3,4,5,7,10 years) in desert irrigation area. Meanwhile, the relationships between soil microbial biomass and soil microbial quantities were studied by correlation analysis, and alfalfa with different planting years was evaluated from the aspect of soil microbes. The results indicated that soil microbial biomass carbon, soil microbial biomass nitrogen, and 3 main categories of soil microorganisms (fungi, bacteria and actinomycetes) decreased significantly with the increase of soil depth; soil microbial biomass carbon, soil microbial biomass nitrogen, bacteria and actinomycetes increased firstly, and then decreased, and then increased with the increase of cropping years; fungi increased, and then decreased with the increase of cropping years. The number of bacteria was the highest (70.72%) and fungi was lowest (0.18%) among the microorganisms. Contrasted with abandoned land, the total microbial count and microbial biomass were all increased in various cropping-year alfalfa field, and were the highest in five-year alfalfa field. The seasonal dynamic of soil microbial biomass and soil microbial quantities was apparent. The maximum number of soil microbial biomass and soil microbial quantities was observed in July, and the minimum in April, except for fungi, which the maximum number was observed in September.

Key words: Desert irrigation, Alfalfa grassland, Soil microbial biomass, Soil microbial quantities, Seasonal change

摘要：

采用熏蒸-提取法、微生物培养法，研究了荒漠灌区不同种植年限紫花苜蓿（Medicago sativa L. ‘Gannong No.3’）草地0～60 cm土层土壤微生物量和数量，从土壤微生物的角度对荒漠灌区苜蓿的退化机理、人工草地管理做出了评价。结果表明，不同种植年限苜蓿草地土壤微生物量碳、氮及微生物（细菌、真菌、放线菌）数量均呈现随土层深度的增加而减小的趋势；随苜蓿种植年限的增加，土壤微生物量碳、氮，细菌和放线菌数量均呈增加-降低-增加的变化趋势，真菌数量呈先增加后降低的变化趋势；土壤微生物群落以细菌占绝对优势（70.72%），真菌最少（0.18%），微生物总数量和微生物生物量均大于撂荒地，且5年生苜蓿地微生物总数最多，是其他各种植年限的1.58～6.17倍，且微生物生物量碳、氮与细菌、放线菌数量呈极显著正相关。微生物生物量及数量表现出明显的季节动态，除土壤真菌数量最大值出现在9月份之外，其余指标最大值均出现在7月份，最小值在4月份。

关键词: 荒漠灌区, 苜蓿草地, 微生物生物量, 微生物数量, 季节变化

CLC Number:

S154.3

NAN Li-li, SHI Shang-li, YU Ji-hua. Soil Microbial Properties in Alfalfa Field with Different Growing Years in Arid Desert Oasis[J]. Acta Agrestia Sinica, 2016, 24(5): 975-980.

南丽丽, 师尚礼, 郁继华. 荒漠灌区不同种植年限苜蓿草地土壤微生物特性[J]. 草地学报, 2016, 24(5): 975-980.

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References

[1] Taylor T P, Wilson B, Mills M S, et al. Comparison of microbial numbers and enzymatic activities in surface soils and sub-soils using various techniques[J]. Soil Biology and Biochemistry,2002,34:387-401
[2] 付为国,王凡坤,李萍萍,等. 铅对小白菜根际微生物群落与其生理功能的影响[J]. 农业机械学报,2014,45(12):203-208
[3] 张宝泉,李红红,马虎,等. 渭北旱塬区不同年限苜蓿对土壤主要养分及微生物量的影响[J]. 草地学报,2015,23(6):1190-1196
[4] 徐丽君,王波,辛晓平. 紫花苜蓿人工草地土壤养分及土壤微生物特性[J]. 草地学报,2011,19(3):406-411
[5] 张萌萌,敖红,张景云,等. 建植年限对紫花苜蓿根际土壤微生物群落功能多样性的影响[J]. 草业科学,2014,31(5):787-796
[6] 麻冬梅,金风霞,蒙静,等. 不同种植年限苜蓿对土壤理化性质、微生物群落和苜蓿品质的影响[J]. 水土保持研究,2013,20(5):29-32
[7] 金风霞,麻冬梅,刘昊焱,等. 不同种植年限苜蓿地土壤环境效应的研究[J]. 干旱地区农业研究,2014,32(2):73-77
[8] 杨恒山,张庆国,刘晶,等. 不同生长年限紫花苜蓿根系及其土壤微生物的分布[J]. 草业科学,2007,24(11):38-41
[9] Vance E D, Brookes P C, Jenkinson D S. An extraction method for measuring soil microbial biomass[J]. Soil Biology and Biochemistry,1987,19(6):703-707
[10] Brookes P C, Andrea L, Pruden G, et al.Chloroformfumigation and the release of soil nitrogen:A rapid direct extraction method to measure microbial biomass nitrogen in soil[J]. Soil Biology and Biochemistry,1985,17(6):837-842
[11] 许光辉,郑洪元. 土壤微生物分析方法手册[M]. 北京:农业出版社,1986:91-110
[12] 中国科学院南京土壤研究所微生物室. 土壤微生物研究法[M]. 北京:科学出版社,1985:40-273
[13] 蒋金平. 黄土高原半干旱丘陵区生态恢复中植被与土壤质量演变关系[D]. 兰州:兰州大学,2008:52-53
[14] 张成娥,陈小丽,郑粉莉. 子午岭林区不同环境土壤微生物生物量与肥力关系研究[J]. 生态学报,1998,18(2):218-222
[15] 杨玉海,蒋平安,翟军,等. 干旱区绿洲苜蓿地土壤微生物特性及其影响因子[J]. 水土保持学报,2008,22(6):153-157
[16] 李延茂,胡江春,汪思龙,等. 森林生态系统中土壤微生物的作用与应用[J]. 应用生态学报,2004,15(10):1943-1946
[17] Humphries J A, Ashe A M H, Smiley J A, et al. Microbial community structure and trichloroethylene degradation in ground water[J]. Canadian Journal of Microbiology,2005,51(6):433-440
[18] 姚槐应,黄昌勇. 土壤微生物生态学及其实验技术[M]. 北京:科学出版社,2006:30-45
[19] 龙章富,刘世贵. 退化草地土壤农化性状与微生物区系研究[J]. 土壤学报,1996,33(2):192-200
[20] 卢虎,李显刚,姚拓,等. 高寒生态脆弱区"黑土滩"草地植被与土壤微生物数量特征研究[J]. 草业学报,2014,23(5):214-222

Soil Microbial Properties in Alfalfa Field with Different Growing Years in Arid Desert Oasis

荒漠灌区不同种植年限苜蓿草地土壤微生物特性

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