Analysis of Cultivable Fungal Community Structure and Spatial Distribution in Desert Steppe, Inner Mongolia, China

doi:10.11733/j.issn.1007-0435.2017.02.014

Acta Agrestia Sinica ›› 2017, Vol. 25 ›› Issue (2): 315-321.DOI: 10.11733/j.issn.1007-0435.2017.02.014

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Analysis of Cultivable Fungal Community Structure and Spatial Distribution in Desert Steppe, Inner Mongolia, China

JIA Mei-qing¹, HUANG Jing², MENG Yuan², HAN Guo-dong³, SUN Sheng³, ZHANG Guo-gang², YANG Ying-hua²

1. Tianjin Key Laboratory of Water Resource and Water Environment, Tianjin Normal University, Tianjin 300387, China;
2. College of Life Sciences, Tianjin Normal University, Tianjin 300387, China;
3. College of Grsaalang, Resources and Environment, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010019, China

Online:2017-04-15 Published:2017-06-25

内蒙古荒漠草原土壤可培养真菌的群落结构和空间分布分析

贾美清¹, 黄静², 孟元², 韩国栋³, 孙胜³, 张国刚², 杨英花²

1. 天津师范大学天津市水资源与水环境重点实验室, 天津 300387;
2. 天津师范大学生命科学学院, 天津 300387;
3. 内蒙古农业大学草原与资源环境学院, 内蒙古呼和浩特 010019

通讯作者: 张国刚,E-mail:zangguogang@163.com
作者简介:贾美清(1978-),女,山西人,硕士,实验师,E-mail:jiameiqing@mail.nankai.edu.cn
基金资助:
国家自然科学基金（31500365，31100330，31270502）；天津市自然科学基金（12JCYBJC19700）；天津市科技支撑基金项目（15ZCZDSF00410）资助

Abstract

Abstract:

In order to assess the community structure and spatial distribution of cultivable fungi in desert steppe, Inner Mongolia, China, dilution plate method and 18S rRNA molecular identification technique were used to study the community composition, distribution and diversity of cultivable fungi. Pearson's correlation was used to analyze the relationships between the quantity of fungi and soil physical and chemical factors. The quantity of cultivable fungi was 26.65×10⁴~90.69×10⁴ CFU·g^-1 in 0~30 cm soil depth. The distribution trend of cultivable fungi was 0~10 cm > 10~20 cm > 20~30 cm. Cultivable fungi isolated from desert steppe soil belonged to 14 genus including Penicillium, Fusarium, and so on. The dominant strains were Penicillium and Aspergillus, which relative abundances in 0~30 cm soil layer were 48.76% and 10%, respectively. Compared with other two soil layers, the species richness and Shannon-Weiner Index(H') of cultivable fungi were the highest in 0~10 cm soil depth. The distribution of cultivable fungi had a significant correlation with water content, NH₄⁺-N, available potassium, and soil organic matter content, while it had a significantly negative correlation with pH value. It had no significant correlation with available phosphorus and NO₃^--N content.

Key words: Desert steppe, Cultivable fungi, 18S rRNA, Community structure, Spatial distribution

摘要：

为了揭示荒漠草原土壤可培养真菌的群落结构和空间分布，以实现利用微生物对环境变化的敏感性对退化草地提前预测和有效管理，采用稀释平板涂布法和18S rRNA分子鉴定技术对内蒙古荒漠草原土壤可培养真菌的群落结构、多样性、空间分布及其与土壤环境因子的相关性进行分析。结果表明：可培养真菌在0~30 cm土层的数量为26.65×10⁴~90.69×10⁴ CFU·g^-1，各土层的真菌分布次序为：0~10 cm > 10~20 cm > 20~30 cm。分离到的真菌分别属于14个属，其中青霉菌属是优势菌属，占0~30 cm可培养真菌总数的48.76%，其次是曲霉属，占可培养真菌总数的10%。在0~10 cm土层，可培养真菌的物种丰富度和Shannon-Wiener 指数（H'）最高。可培养真菌的数量与含水量、速效钾和铵态氮呈显著正相关关系（P<0.05）；与有机质含量呈极显著正相关关系（P<0.01），与pH值呈显著负相关关系（P<0.05）；与硝态氮和速效磷无显著相关性。

关键词: 荒漠草原, 可培养真菌, 18S rRNA, 多样性, 空间分布

CLC Number:

S154.3

JIA Mei-qing, HUANG Jing, MENG Yuan, HAN Guo-dong, SUN Sheng, ZHANG Guo-gang, YANG Ying-hua. Analysis of Cultivable Fungal Community Structure and Spatial Distribution in Desert Steppe, Inner Mongolia, China[J]. Acta Agrestia Sinica, 2017, 25(2): 315-321.

贾美清, 黄静, 孟元, 韩国栋, 孙胜, 张国刚, 杨英花. 内蒙古荒漠草原土壤可培养真菌的群落结构和空间分布分析[J]. 草地学报, 2017, 25(2): 315-321.

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References

[1] 谭红妍,陈宝瑞,闫瑞瑞,等. 草地土壤微生物特性及其对人为干扰响应的研究进展[J]. 草地学报,2014,22(6):1163-1170
[2] Rosa M, Melanie J, Dagmar T, et al. Microbial communities and activities in alpine and subalpine soils[J]. Microbiological Ecology,2009,67:208-218
[3] Zhang J Q, Han S J, Zhou Y M. Microbial activity in a temperate forest soil as affected by elevated atmospheric CO₂[J]. Pedosphere,2010,20:427-435
[4] 张瑞娟,李华,林勤保,等. 土壤微生物群落表征中磷脂脂肪酸(PLFA)方法研究进展[J]. 山西农业科学,2011,39(9):1020-1024
[5] Bissett A, Burke C. Bacterial community shifts in organically perturbed sediments[J].Environmental Microbiology,2007,9(1):46-60
[6] Griffiths B S, Ritz K, Bardgett R D, et al. Ecosystem response of pasture soil communities to fumigation-induced microbial diversity reductions:An examination of the biodiversity-ecosystem function relationship[J]. Oikos-A Journal of Ecology,2000,90(2):279-294
[7] Hawksworth D L. The magnitude of fungal diversity:The 1.5 million species estimate revisited[J]. Mycological Research, 2001,105:1422-1432
[8] 韦红群,邓建珍,曹建华,等. 柱花草根系与根际微生物类群的研究[J]. 草业科学,2009,26(1):69-73
[9] 南丽丽,师尚礼,郁继华. 荒漠灌区不同种植年限苜蓿草地土壤微生物特性[J]. 草地学报,2016,24(5):975-980
[10] Kjoller A, Struwe S. Microfungi in ecosystems:Fungal occurrence and activity in litter and soil[J]. Oikos,1982,39(3):389-422
[11] 中国科学院内蒙古宁夏综合考察队. 内蒙古植被[M]. 北京:科学出版社,1985:405-408
[12] 李德新. 短花针茅荒漠草原动态规律及其生态稳定性[J]. 中国草地,1994,(4):1-5
[13] 韩芳,牛建明,刘朋涛,等. 气候变化对内蒙古荒漠草原牧草气候生产力的影响[J]. 中国草地学报,2010,32(5):57-65
[14] Yu M, Ellis J E, Epstein H E. Regional analysis of climate, primary production, and livestock density in Inner Mongolia[J]. Journal of Environmental Quality,2004,33(5):1675-1681
[15] 韩芳. 气候变化对内蒙古荒漠草原生态系统的影响[D]. 呼和浩特:内蒙古大学,2013:12-13
[16] 李元恒. 内蒙古荒漠草原植物群落结构和功能对增温和氮素添加的响应[D]. 呼和浩特:内蒙古农业大学,2014:9
[17] 刘涛,张永贤,许振柱,等. 短期增温和增加降水对内蒙古荒漠草原土壤呼吸的影响[J]. 植物生态学报,2012,36(10):1043-1053
[18] 吴永胜,马万里,李浩,等. 内蒙古退化荒漠草原土壤有机碳和微生物生物量碳含量的季节变化[J]. 应用生态学报,2010,21(2):312-316
[19] 格根宝乐尔. 放牧制度与放牧强度对内蒙古短花针茅荒漠草原AM真菌多样性的影响[D]. 呼和浩特:内蒙古大学,2013:8
[20] 荣丽,李贤伟,朱天辉,等. 光皮桦细根与扁穗牛鞭草草根分解的土壤微生物数量及优势类群[J]. 草业学报,2009,18(4):117-124
[21] 王晨晨,王珍,张新杰,等. 增温对荒漠草原植物群落组成及物种多样性的影响[J]. 生态环境学报,2014,23(1):43-49
[22] Lu R K. Methods of Soil Analysis[M]. Beijing:Chinese Agricultural Press,2000:30-72
[23] 钱存柔,黄仪秀. 微生物学实验教程[M]. 北京:北京大学出版社,1999:98
[24] 朱英波,史凤玉,王牛牛,等. 黑龙江大豆胞囊线虫胞囊可培养细菌多样性[J]. 微生物学报,2012,52(7):902-909
[25] Brookes P C, Powlson D S, Jenkinson D S. Measurement of microbial biomass phosphorus in soil[J]. Soil Biology and Biochemistry,1984,16(2):169-175
[26] 张俊忠,陈秀蓉,杨成德,等. 东祁连山高寒草地土壤可培养真菌多样性分析[J]. 草业学报,2010,19(2):124-132
[27] 刘增文,段而军,高文俊,等. 秦岭山区人工林地枯落叶客置对土壤生物化学性质的影响[J]. 应用生态学报,2008,19(4):704-710
[28] 潘好芹,张天宇,黄悦华,等. 太白山土壤淡色丝孢真菌群落多样性及生态位[J]. 应用生态学报,2009,20(2):363-369
[29] 丁玲玲. 东祁连山不同高寒草地土壤微生物数量分布特征研究[J]. 农业环境科学学报,2007,26(6):2104-2111
[30] 赵吉,廖仰南,张桂枝,等. 草原生态系统的土壤微生物生态[J]. 中国草地,1999,21(3):57-67
[31] 朱瑞芬,唐凤兰,张月学,等. 不同利用方式对东北羊草草地土壤微生物数量的影响[J]. 草地学报,2012,20(5):842-847
[32] 姚贤民,吕国忠,杨红,等. 长白山森林土壤真菌区系研究[J]. 菌物研究,2007,5(1):43-46
[33] Pan H Q, Yu J F, Wu Y M, et al. Diversity analysis of soil dematiaceous hyphomycetes from the Yellow River source area[J]. Journal of Zhejiang University Science B,2008,9:829-834
[34] 刘峰. 红树林可培养微生物活性评价和土壤宏基因组文库构建及生物活性蹄选[D]. 儋州:华南热带农业大学,2006:13
[35] 陆俊锟. 华南三地红树林土壤微生物区系及PGPR多样性研究[D]. 南宁:广西大学,2008:7

Analysis of Cultivable Fungal Community Structure and Spatial Distribution in Desert Steppe, Inner Mongolia, China

内蒙古荒漠草原土壤可培养真菌的群落结构和空间分布分析

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