Effects of Salicylic Acid on Physiological Characteristics and Growth of Alfalfa Seedling under NaCl Stress

doi:10.11733/j.issn.1007-0435.2017.06.016

Acta Agrestia Sinica ›› 2017, Vol. 25 ›› Issue (6): 1265-1273.DOI: 10.11733/j.issn.1007-0435.2017.06.016

Previous Articles Next Articles

Effects of Salicylic Acid on Physiological Characteristics and Growth of Alfalfa Seedling under NaCl Stress

LU Ya-ling, WANG Ling-jing, WANG Ning, SHAO Chun-lai, LI Zhi-hua

College of Prataculture Science, Nanjing Agricultural University, Nanjing, Jiangsu Province 210095, China

Received:2016-11-24 Revised:2018-01-08 Online:2017-12-15 Published:2018-03-30

外源水杨酸对NaCl胁迫下紫花苜蓿幼苗生长和生理特性的影响

逯亚玲, 王灵婧, 王宁, 邵春来, 李志华

南京农业大学草业学院, 江苏南京 210095

通讯作者: 李志华,E-mail:lizhihua@njau.edu.cn
作者简介:逯亚玲(1994-),女,青海海东人,硕士,E-mail:2015120003@njau.edu.cn
基金资助:
国家大学生创新创业训练计划项目（201610307038）资助

Abstract

Abstract:

In order to ascertain the effects of salicylic acid on physiological characteristics and growth of Alfalfa (Medicago sativa) seedling under NaCl stress, a pot experiment of ‘Weston’ alfalfa cultivars was established. In the study, the effects of different concentrations of exogenous salicylic acid (control 0, 0.5, 1.5, and 2.5 mmol·L) with foliar spray on alfalfa seedling growth and physiological under the condition of different NaCl stress (0, 0.3%, 0.6%, 0.9%, 1.2%) were studied. The results indicated that as the aggravation of the salt stress alfalfa seedling growth was significantly inhibited, while adding exogenous 0.5 and 1.5 mmol·L^-1 salicylic acid significantly improved plant height, stem diameter, number of primary branches, and leaf relative water content under 0.6%, 0.9% and 1.2% salt stress. Moreover salicylic acid applications markedly increased the activities of protected enzymes such as superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), and decreased leaf relative conductivity and the content of malondialdehyde (MDA). Thus it can be seen that treatments with an appropriate concentration of salicylic acid can improve alfalfa growth and enhance its salt tolerance.

Key words: Alfalfa, Salt stress, Salicylic acid

摘要：

为了探明水杨酸对NaCl胁迫下紫花苜蓿（Medicago sativa）幼苗生长和生理特性的影响，以“威斯顿”紫花苜蓿品种为材料，采用盆栽试验方法，研究叶面喷施不同浓度水杨酸（0，0.5，1.5，2.5 mmol·L^-1）对不同NaCl处理（0、0.3%、0.6%、0.9%、1.2%）下紫花苜蓿苗期生长及抗性生理的影响。结果表明，随着NaCl浓度的增加，紫花苜蓿幼苗生长受到显著抑制，在0.6%、0.9%和1.2%盐浓度胁迫下，添加0.5、1.5 mmol·L^-1外源水杨酸后，紫花苜蓿的株高、茎粗、一级分枝数、叶片相对含水量均显著提高（P<0.05）；同时，叶片中的超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）活性显著升高；相对电导率、丙二醛（MDA）含量显著降低（P<0.05）。可见，适宜浓度水杨酸可以改善不同浓度NaCl胁迫下紫花苜蓿的生长状况，提高其耐盐能力。

关键词: 紫花苜蓿, 盐胁迫, 水杨酸

CLC Number:

S541

LU Ya-ling, WANG Ling-jing, WANG Ning, SHAO Chun-lai, LI Zhi-hua. Effects of Salicylic Acid on Physiological Characteristics and Growth of Alfalfa Seedling under NaCl Stress[J]. Acta Agrestia Sinica, 2017, 25(6): 1265-1273.

逯亚玲, 王灵婧, 王宁, 邵春来, 李志华. 外源水杨酸对NaCl胁迫下紫花苜蓿幼苗生长和生理特性的影响[J]. 草地学报, 2017, 25(6): 1265-1273.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2017.06.016

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2017/V25/I6/1265

References

[1] 张永锋, 梁正伟, 隋丽, 等. 盐碱胁迫对苗期紫花苜蓿生理特性的影响[J]. 草业学报, 2009, 18(4):230-235
[2] 马献发, 张继舟, 宋凤斌. 植物耐盐的生理生态适应性研究进展[J]. 科技导报, 2011, 29(14):76-79
[3] 景艳霞, 袁庆华. NaCl胁迫对苜蓿幼苗生长及不同器官中盐离子分布的影响[J]. 草业学报, 2011, 20(2):134-139
[4] 周和平, 张立新, 禹锋, 等. 我国盐碱地改良技术综述及展望[J]. 现代农业科技, 2007(11):159-161
[5] 王占升, 朱汉, 一前宣正. 牧草耐盐力及盐碱地引种试验[J]. 中国草地学报, 1995(2):38-42
[6] 谢振宇, 杨光穗. 牧草耐盐性研究进展[J]. 草业科学, 2003, 20(8):11-17
[7] 曹宏, 章会玲, 盖琼辉, 等. 22个紫花苜蓿品种的引种试验和生产性能综合评价[J]. 草业学报, 2011, 20(6):219-229
[8] 王珺, 柳小妮. 3个紫花苜蓿品种耐盐突变材料的耐盐性评价[J]. 草业科学, 2011, 28(1):79-84
[9] 吴欣明, 王运琦, 刘建宁, 等. 羊茅属植物耐盐性评价及其对盐胁迫的生理反应[J]. 草业学报, 2007, 16(6):67-73
[10] 王鑫, 马永祥, 李娟. 紫花苜蓿营养成分及主要生物学特性[J]. 草业科学, 2003, 20(10):39-41
[11] 杨跃霞. 盐胁迫下紫花苜蓿的生理机制研究[D]. 扬州:扬州大学, 2010
[12] Raskin I. Role of Salicylic Acid in Plants[J]. Annual Review of Plant Biology, 2003, 43(43):439-463
[13] Fragnière C, Serrano M, Abou-Mansour E, et al. Salicylic Acid and Its Location in Response to Biotic and Abiotic Stress[J]. Febs Letters, 2011, 585(12):1847-1852
[14] Ayyub C M, Ali M, Shaheen M R, et al. Enhancing the Salt Tolerance Potential of Watermelon (Citrullus lanatus) by Exogenous Application of Salicylic Acid[J]. American Journal of Plant Sciences, 2015, 6(19):3267-3271
[15] Yuan S, Lin H H. Role of Salicylic Acid in Plant Abiotic Stress[J]. Zeitschrift Für Naturforschung C Journal of Biosciences, 2008, 63(5-6):313-320
[16] Doulatabadian A, Modares S, Etemadi F. Effect of Pretreatment of Salicylic Acid on Wheat (Triticum aestivum L.) Seed Germination Under Salt Stress[J]. Jranian Journal of Biology, 2008, 21(4):692-702
[17] 常云霞, 李青芝, 杜红阳, 等. 水杨酸对盐胁迫下小麦幼苗生长抑制的缓解效应[J]. 安徽农业科学, 2009, 37(4):1428-1429
[18] Hausrath A C. Effect of Growth Regulators on Growth, Yield and Ions Accumulation of Rice (Oryza sativa L.) Under Salt Stress[J]. Pakistan Journal of Botany, 2006, 15(9):2051-2061
[19] Esmaeili M, Heidarzade A. Enhance the Allelopathic Potential of Two Rice Cultivar (Oryza sativa L.) by Foliar Application of Salicylic Acid Under Salinity Stress[J]. International Journal of Biosciences, 2015, 6(4):177-183
[20] Xin h h, Li Z, Li F, et al. effects of exogenous regulating substances on physiological traits of cold tolerance in cotton seedling[J]. Cotton Science, 2015, 27(3):254-259
[21] 张红. 硝普钠、24-表油菜素内酯-水杨酸浸种对盐胁迫下玉米种子萌发及幼苗生长的影响[J]. 核农学报, 2012, 26(1):164-169
[22] 王玉萍, 董雯, 张鑫, 等. 水杨酸对盐胁迫下花椰菜种子萌发及幼苗生理特性的影响[J]. 草业学报, 2012, 21(1):213-219
[23] 刘杰, 杨絮茹, 周蕴薇. 水杨酸浸种处理对黑麦草种子萌发及幼苗抗旱性的影响[J]. 草业科学, 2011, 28(4):582-585
[24] Agarwal S, Sairam R K, Srivastava G C, et al. Changes in antioxidant enzymes activity and oxidative stress by abscisic acid and salicylic acid in wheat genotypes[J]. Biologia Plantarum, 2005, 49(4):541-550
[25] 王学奎. 植物生理生化实验原理和技术[M]. 北京:高等教育出版社, 2006
[26] 李合生. 植物生理生化实验原理和技术[M]. 北京:高等教育出版社, 2000
[27] 张志良, 瞿伟菁. 植物生理学实验指导[M]. 3版. 北京:高等教育出版社, 2003
[28] 孙小芳, 郑青松, 刘友良. NaCl胁迫对棉花种子萌发和幼苗生长的伤害[J]. 植物资源与环境学报, 2000, 9(3):22-25
[29] 单长卷, 赵新亮, 汤菊香. 水杨酸对干旱胁迫下小麦幼苗抗氧化特性的影响[J]. 麦类作物学报, 2014, 34(1):91-95
[30] Munns R. Physiological processes limiting plant growth in saline soils:some dogmas and hypotheses[J]. Plant Cell and Environment, 1993, 16(1):15-24
[31] 周万海, 师尚礼, 寇江涛. 外源水杨酸对苜蓿幼苗盐胁迫的缓解效应[J]. 草业学报, 2012, 21(3):171-176
[32] 徐芬芬, 叶利民, 张勇, 等. 水杨酸对小白菜幼苗抗盐性的诱导效应[J]. 亚热带植物科学, 2010, 39(3):15-17
[33] 崔秀妹, 刘信宝, 李志华, 等. 不同水分胁迫下水杨酸对分枝期扁蓿豆生长及光合生理的影响[J]. 草业学报, 2012, 21(6):82-93
[34] André D A, José T, Joaquim E, et al. Effect of salt stress on antioxidative enzymes and lipid peroxidation in leaves and roots of salt-tolerant and salt-sensitive maize genotypes[J]. Environmental and Experimental Botany, 2006, 56(1):87-94
[35] 马占青. 水杨酸在黑果枸杞抗盐性中的作用[D]. 兰州:兰州大学, 2013
[36] 周旋. 盐胁迫下茶树对外源水杨酸的生理响应[D]. 杨凌:西北农林科技大学, 2014
[37] 黄玉梅, 张杨雪, 刘庆林, 等. 水杨酸对盐胁迫下百日草种子萌发及幼苗生理特性的影响[J]. 草业学报, 2015, 24(7):97-105
[38] 朱伟, 房卫平, 谢德意, 等. 水杨酸对NaCl胁迫下抗虫棉幼苗生长和生理特性的影响[J]. 河南农业大学学报, 2012, 46(5):515-519
[39] 王东明, 贾媛, 崔继哲. 盐胁迫对植物的影响及植物盐适应性研究进展[J]. 中国农学通报, 2009, 25(4):124-128
[40] 徐芬芬, 叶利民, 潘维华. 外源水杨酸对盐胁迫下水稻幼苗生长的影响[J]. 广东农业科学, 2009(9):22-24
[41] 王俊斌, 王海凤, 刘海学. 水杨酸促进盐胁迫条件下水稻种子萌发的机理研究[J]. 华北农学报, 2012, 27(4):223-227
[42] 朱伟, 袁超, 马宗斌, 等. 水杨酸对盐胁迫下棉花种子萌发和幼苗生长的影响[J]. 江西农业学报, 2009, 21(10):17-19
[43] 宿越, 李天来, 杨凤军, 等. 外源水杨酸对NaCl胁迫下番茄幼苗保护酶活性和渗透调节物质含量的影响[J]. 沈阳农业大学学报, 2009, 40(3):273-276
[44] 曾长立, 陈禅友. 外源水杨酸降低辣椒幼苗盐害的生理效应[J]. 东北农业大学学报, 2010, 41(11):32-37

Effects of Salicylic Acid on Physiological Characteristics and Growth of Alfalfa Seedling under NaCl Stress

外源水杨酸对NaCl胁迫下紫花苜蓿幼苗生长和生理特性的影响

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Jing, LIU Xiao-jing, Cheng Tian-tian, Tong Chang-chun, WANG Xue. Study on Leaf Characteristics and Yield Effect of Alfalfa with Different Nitrogen Efficiency [J]. Acta Agrestia Sinica, 2021, 29(9): 1941-1949.
[2]	MENG Xuan-chen, MA Peng-cheng, MA Jie, DUAN Zhen, HAN Yang-yang, ZHANG Ji-yu. Study on the Effect of Mulching Furrow and Fertilization of Alfalfa in Semi-arid Areas of Loess Plateau [J]. Acta Agrestia Sinica, 2021, 29(9): 2098-2106.
[3]	CHEN Lei, BAO Xue-yan, GUO Gang, HUO Wen-jie, LI Qing-hong, XU Qing-fang, WANG Cong, LIU Qiang. Effects of Tannic Acid and Lactic Acid Bacteria on Alfalfa Silage Quality and Ruminal Fermentation in Vitro [J]. Acta Agrestia Sinica, 2021, 29(8): 1853-1858.
[4]	LI Chun-pin, QIU Yi-hui, XIA Hou-yin, TAN Lu-na, XU Huan-yu, ZHANG Han-wen, GAO Jing-hui. Membership Function Analysis of Low Transpiration and Yield Traits in Alfalfa Multi-paternal Hybrid F₁Generation [J]. Acta Agrestia Sinica, 2021, 29(7): 1416-1422.
[5]	ZHANG Wei-yu, WANG Chun-yong, DU Hong-mei. Effects of Hydrogen-rich Water on Salt-Tolerance of Kentucky Bluegrass and Antioxidant Enzymes Activity [J]. Acta Agrestia Sinica, 2021, 29(7): 1436-1445.
[6]	LI Tian-qi, LIN Zhi-ling, LU Xuan, HUANG Jia-yuan, YANG Ning, WO Ye, GAO Kai, LIU Xiao-ni, WANG Xian-guo. Effects of Irrigation Amount on Soil Available Nutrient Distribution and Leaching Loss of Alfalfa in Horqin Sandy Land [J]. Acta Agrestia Sinica, 2021, 29(7): 1454-1461.
[7]	LI Hong, LI Bo, FANG Zhi-jian, YANG Zhao, ZHANG Chao, FENG Cheng-long, JIN Shan-shan, WANG Si-qi, SHI Wan-ying. Physiological Response of Alfalfa Seedlings to Freeze-thaw or Freeze-thaw Versus Soda Saline-alkali Stress [J]. Acta Agrestia Sinica, 2021, 29(7): 1469-1476.
[8]	LI Peng-zhen, ZHAO De-qin, DENG Bo, Yang Jun, Zhao Guo-hua, Zhao Liang. Effects of Biochar Addition and Drought Stress on Photosynthetic Efficiency and Growth of Alfalfa Inoculated with Rhizobia [J]. Acta Agrestia Sinica, 2021, 29(6): 1257-1264.
[9]	WANG Wen-jing, MA Dong-mei, ZHAO Li-juan, MA Qiao-li. Effects of 2,4-table Brassinolide on Enzyme Activity and Root ion Distribution and Absorption in Alfalfa Seedlings [J]. Acta Agrestia Sinica, 2021, 29(6): 1363-1368.
[10]	SUN Lei-lei, ZHAO Gui-qin, CHAI Ji-kuan, LIU Fu-yuan, Nie Xiumei, WANG Jun. Effects of Storage Methods and Time on Mould Quantity and Species in Alfalfa Hay [J]. Acta Agrestia Sinica, 2021, 29(5): 884-893.
[11]	HUANG Rong, YAO Bo, ZHANG Yu-juan, JIA Qian-ying, LI Xiang-yang, ZHANG Hong, ZHANG Zhen-fen. Effect of Pantoea agglomerans CQ10 Lipopolysaccharide on Seed Germination and Seedling Growth of Alfalfa [J]. Acta Agrestia Sinica, 2021, 29(5): 965-971.
[12]	CHEN Wei-dong, ZHANG Yu-xia, CONG Bai-ming, A Ma-er, TIAN Yong-lei, ZHANG Qing-xin, DU Xiao-yan. Effects of Potassium Fertilizer on MDA,SP Content and Antioxidant System of Alfalfa Root Neck [J]. Acta Agrestia Sinica, 2021, 29(4): 717-723.
[13]	XU Hong-yu, ZHEN Li-li, LI Yu-ying, DONG Kuan-hu, LI Xiang-lin. Effect of Freeze-drying Environment on Freezing Tolerance of Alfalfa Crowns [J]. Acta Agrestia Sinica, 2021, 29(4): 724-733.
[14]	LI Man-you, MA Zhong-ren, WANG Bing, YANG Yu-qi, SHEN Xiao-tian, CAO Li-juan, LI Xiao-yun, LAN Jian. Study on the Mixed Planting Mode of Two Alfalfa Varieties in Different Row Ratios in the Yellow River Irrigation Area in Ningxia [J]. Acta Agrestia Sinica, 2021, 29(4): 798-804.
[15]	HOU Hong-yan, SHEN Chen, HUO Wen-jie, LIU Qiang, ZHANG Shuan-lin, WANG Cong, CHEN Lei, XU Qing-fang, WANG Yong-xin, WANG Zhong-hao, GUO Gang. The Effects of Homofermentative Lactobacillus on Ensiling Characteristics and in Vitro Digestibility of Low-moisture Alfalfa Silage [J]. Acta Agrestia Sinica, 2021, 29(4): 835-841.