Effects of Sonication Treatment on Seed Germination of Dactylis glomerata and Agropyron cristatum under Cadmium Stress

doi:10.11733/j.issn.1007-0435.2018.02.022

Acta Agrestia Sinica ›› 2018, Vol. 26 ›› Issue (2): 427-434.DOI: 10.11733/j.issn.1007-0435.2018.02.022

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Effects of Sonication Treatment on Seed Germination of Dactylis glomerata and Agropyron cristatum under Cadmium Stress

JIAN Ni¹, LIU Lu¹, QU Jia-xin¹, LI Kai¹, WANG Quan-zhen¹

1. College of Animal Science and Technology, Northwest A & F University, Yangling, Shaanxi Province 712100, China

Received:2017-07-17 Revised:2018-03-20 Online:2018-04-15 Published:2018-07-05

超声波对镉胁迫鸭茅和冰草种子萌发的影响

见妮¹, 刘璐¹, 屈佳欣¹, 李凯¹, 王佺珍¹

1. 西北农林科技大学动物科技学院, 陕西杨凌 712001

通讯作者: 王佺珍
作者简介:见妮(1996-),女,汉族,在读本科生,E-mail:1974312430@qq.com
基金资助:
国家自然科学基金（31472138）和大学生科创项目资助

Abstract

Abstract:

In order to investigate the effects of sonication and cadmium on germination rate, plumule length and physiological activity of Dactylis glomerata L. and Agropyron cristatum L., seeds were both individual and combinatively treated with sonication and cadmium at 5 processing levels, respectively. The treated seeds were germinated on Petri dishes for 20 days. The results showed that under the sonication treatment,the germination rates of D. glomerata and A. cristatum seeds were improved and reached the highest at 5 min of treatment when the activity of POD and the content of MDA in D. glomerata seedlings were significantly increased. The five different concentration of cadmium can inhibit the germination rate and plumule length of two grasses except that the cadmium concentration of 0.04%. As for D.glomerata, the cadmium concentration of 0.02% can improve POD activity significantly, but had no significant effect on the content of MDA. Under the combined treatments of sonication and cadmium stress, the germination rate of D.glomerata seeds was improved significantly at 0.05% of cadmium and 10 min of sonication, so did in A. cristatum. The plumule length of D. glomerata was improved by sonication treatment and under 0.01% of cadmium. Overall, sonication treatment effectively mitigated injury in cadmium stress in the tested two plants, therefore, significantly enhanced the seed vigour and seedling growth.

Key words: Dactylis glomerata, Agropyron cristatum, Cadmium stress, Sonication, Germination rata, Plumule length, POD activity, MAD content

摘要：

为探讨超声波和重金属镉二因素对鸭茅（Dactylis glomerata L.）和冰草（Agropyron cristatum L.）种子发芽率、胚芽生长和生理活性的影响，设置五种不同时长的超声波处理两种种子，处理后的种子分别用不同浓度的氯化镉溶液进行处理，通过培养皿纸上发芽法培养20d。结果表明：单一超声波处理，均提高鸭茅种子的发芽率且在5 min时发芽率最高，冰草种子仅在超声波5 min的处理下发芽率提高；超声波时长为5 min时显著提高鸭茅幼苗中POD的活性；各超声波处理均使鸭茅幼苗中MDA含量增加。单一镉溶液处理均抑制鸭茅和冰草种子的发芽和胚芽的生长（除浓度为0.04%的镉溶液提高冰草种子的发芽率）；0.02%镉显著提高鸭茅幼苗POD的活性，镉溶液对鸭茅幼苗中MDA的含量无显著作用。超声波与镉溶液组合处理下，在0.05%的镉胁迫下，超声波处理10 min显著提高鸭茅和冰草种子的发芽率；当镉溶液浓度为0.01%时，各超声波显著促进鸭茅胚芽生长。综合来看，超声波处理可以有效减缓鸭茅和冰草种子的镉胁迫效应，提高种子和成苗活力。

关键词: 鸭茅, 冰草, 镉胁迫, 超声波, 发芽率, 胚芽, POD活性, MDA含量

CLC Number:

JIAN Ni, LIU Lu, QU Jia-xin, LI Kai, WANG Quan-zhen. Effects of Sonication Treatment on Seed Germination of Dactylis glomerata and Agropyron cristatum under Cadmium Stress[J]. Acta Agrestia Sinica, 2018, 26(2): 427-434.

见妮, 刘璐, 屈佳欣, 李凯, 王佺珍. 超声波对镉胁迫鸭茅和冰草种子萌发的影响[J]. 草地学报, 2018, 26(2): 427-434.

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References

[1] 马彦. 土壤重金属污染及其植物修复研究综述[J]. 甘肃农业科技,2016,02:69-75
[2] Muhammad Adrees,Shafaqat Ali,Muhammad Rizwan. Mechanisms of silicon-mediated alleviation of heavy metal toxicity in plants;A review[J]. Ecotoxicology and Environmental Safety,2015:186-197
[3] Ceyda Ozfidan-Konakci,Evren Yildiztugay,Mustafa Bahtiyar. The humic acid-induced changes in the water status,chlorophyⅡ fluorescene and antioxidant defense system of wheat leaves with cadmium stress[J]. Ecotoxicology and Environmental Safety,2018,7:66-75
[4] Xingxing Zhang,Haiyun Rui,Fenqin Zhang. Overexpression of a Functional Vicia sativa PCS1 Homolog increases cadmium tolerance and phytochelatins synthesis in Arabidopsis[J]. Frontiers in Plant Science,2018.9:107
[5] Dora Ⅱ,Gary G,Schwartz. Cadmium and renal cancer[J]. Toxicology and Applied Pharmacology,2005,207:179-186
[6] 张杏锋,夏汉平,李志安,等. 牧草对重金属污染土壤的植物修复综述[J]. 生态学杂志,2009,08:1640-1646
[7] Guaranty Feng,Linkai Huang,Ji Li,et al. Comprehensive transcriptome analysis reveals distinct regulatory programs during vernalization and floral bud development of orchardgrass(Dactylis glomerata L.)[J]. BMC Plant Biology,2017,11:216
[8] 彭燕,张新全. 鸭茅种质资源多样性研究进展[J]. 植物遗传资源学报,2003,02:179-183
[9] 朱选伟,黄振英,张淑敏,等. 浑善达克沙地冰草种子萌发、出苗和幼苗生长对土壤水分的反应[J]. 生态学报,2005,02:364-370
[10] Marin A,Lopez-Gonzalvez A,Barbas C. Development and validation of extraction methods for determination of Zinc and arsenic speciation in soils using focused ultrasound-Application to heavy metal study in mud and soils[J]. Analytica Chimica Acta,2001,442(2):305-318
[11] Shin Y K,Baque M A,Elghamedi S,et al. Effects of activated charcoal, plant growth regulators and ultrasonic pre-treatments on in vitro germination and protocorm formation of Calanthe hybrids[J]. Australian J Crop Sci 5:582-588
[12] Goussous S J,Samarah N H,Alqudah A M,et al. Enhancing seed germination of four crop species using an ultrasonic technique[J]. Exp Agri,46:231-242
[13] Scouten A J,Beuchat L R. Combined effects of chemical, heat and ultrasound treatments to kill Salmonella and Escherichia on alfalfa seeds[J]. J Appl Microb,92:668-674
[14] Yaldagard M,Mortazavi S A,Tabatabaie F. Influence of ultrasonic stimulation on the germination of barley seed and its alpha-amylase activity[J]. Afr J Biotechnol 7:2465-2471
[15] Jia Luo,Zhen Fang,Richard L S. Ultrasound-enhanced conversion of biomass to bilfuels[J]. Progress in Energy and Combustion Science.,2014,41:56-93
[16] Mahmoun Karimi,Bryan Jenkins,Pieter Stroeve. Ultrasound inradiation in the production of ethanol from biomass[J]. Renewable and Sustainable Energy Reviews,2014,40:400-421
[17] 高俊凤. 植物生理学实验指导[M]. 北京:高俊凤,2006:217-218
[18] 秀花. 放牧胁迫下冰草叶片内保护酶活性及丙二醛含量的变化[J]. Agricultural Science&Technology,2015,01:22-24
[19] 刘晓英,孙荣喜,许彬等. 利用超声波处理林木种子的试验[J]. 林业科技,1994,05:7-8
[20] 张文明,赵志杰,刘铂,等. 超声波不同剂量处理水稻种子对其发芽出苗的影响[J]. 种子,1993,05:48
[21] 庄南生,王英,唐燕琼,等. 超声波处理柱花草种子的生物学效应研究[J]. 草业科学,2006,03:80-82
[22] Wang Quanzhen,Chen Guo,Hayixia Yersaiyiti,et al. Modeling analysis on germination and seedling growth using ultrasound seed pretreatment in switchgrass[J]. Plos One,2012,7(10):e47204
[23] R B V Huystee. Some Molecular Aspects of Plant Peroxidase Biosynthetic Studies[J]. Annual Review of Plant Physiology,1987(38):205-219
[24] I Yu,Sakharov, M K Vesga B,Galaev,et al. Peroxidase from leaves of royal palm tree Roystonea regia:purification and some properties[J]. Plant Science,2001,161:853-860
[25] 孙立军,徐彤,任跃英,等. 超声处理对人参种子萌发以及SOD、POD的影响[J]. 中国现代中药,2012,01:24-28
[26] Yasuo Yamauchi a,Ai Furutera a,Kumiko Seki a,et al. Malondialdehyde generated from peroxidized linolenic acid causes protein modification in heat-stressed plants[J]. Plant Physiology and Biochemistry,2008,46:786-793
[27] 赵奇,李玉华,杨玉珍,等. 油用牡丹丹皮液对青椒丙二醛含量及抗氧化酶活性的影响[J]. 北方园艺,2015,05:144-147
[28] 何俊瑜,任艳芳,任明见等. 镉对小麦种子萌发、幼苗生长及抗氧化酶活性的影响[J]. 华北农学报,2009,05:135-139
[29] 龙芝友,高祚文,李剑. 镉污染对高羊茅和多年生黑麦草种子发芽率的影响研究[J]. 农技服务,2009,01:169-170
[30] 郑爱珍,刘传平,沈振国. 镉处理下青菜和白菜MDA含量、POD和SOD活性的变化[J]. 湖北农业科学,2005,01:67-69
[31] 樊明琴,李焰焰,苗杰,等. 镉胁迫对小麦种子萌发和幼苗生长的影响[J]. 安徽农业科学,2010,26:14239-14241
[32] 陈维,金晨钟,吴晓峰,等. 不同浓度镉胁迫对稗草生理特性的影响[J]. 亚热带植物科学,2016,03:207-211
[33] 董汇泽,胡德旺,杨君丽,等. 超声波+赤霉素对柴胡种子萌发及活力的影响[J]. 种子,2006,03:66-67
[34] 史锋厚,朱灿灿,沈永宝,等. 超声波与渗透调节处理对油松种子萌发的影响[J]. 浙江农林大学学报,2011,04:545-549
[35] 李妹娟,唐湘如,聂俊,等. 在盐胁迫下超声波处理对籼稻种子萌发的影响[J]. 西南农业学报,2014,06:2440-2443

Effects of Sonication Treatment on Seed Germination of Dactylis glomerata and Agropyron cristatum under Cadmium Stress

超声波对镉胁迫鸭茅和冰草种子萌发的影响

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