Acta Agrestia Sinica

Acta Agrestia Sinica ›› 2018, Vol. 26 ›› Issue (3): 718-726.DOI: 10.11733/j.issn.1007-0435.2018.03.026

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Response of Fatty Acids in Leaves of Poa Pratensis L. to Low Temperature in Autumn

ZHANG Yong-mei1, WEI Jin-mei1, BAI Xiao-ming2, GONG Liang-jian3   

  1. 1. Gansu Provincial Key Laboratory of Aridland Crop Science, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China;
    2. Pratacultural College, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China;
    3. College of Horticulture, Gansu Agricultural University, Lanzhou, Gansu Province 730070, China
  • Received:2017-12-06 Revised:2018-06-04 Online:2018-06-15 Published:2018-08-24

草地早熟禾叶片脂肪酸对秋季低温的响应

张咏梅1, 魏晋梅1, 白小明2, 龚良建3   

  1. 1. 甘肃农业大学 甘肃省干旱生境作物学重点实验室, 甘肃 兰州 730070;
    2. 甘肃农业大学草业学院, 甘肃 兰州 730070;
    3. 甘肃农业大学园艺学院, 甘肃 兰州 730070
  • 通讯作者: 白小明
  • 作者简介:张咏梅(1974-),女,甘肃武威人,博士,副研究员,主要从事草业科学与植物生理学研究,E-mail:zhangyongm@gsau.edu.cn
  • 基金资助:

    国家自然科学基金（31560667）资助

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Abstract:

In order to explore the cold tolerance mechanism of cool-seasom turfgrass, the fatty acids in leaf of Poa pratensis L. in autumn were detected by using gas chromatograph-mass spectrum(GC-MS), and compared with those in midsummer. Twenty-seven kinds of saturated fatty acids were found in the NIST97 library. In which 13 kinds of saturated fatty acidsaccounted for about 17%, including palmitic acid (about 14%) and stearic acid (about 1.4%). The others 14 kinds of fatty acid were unsaturated, accounting for about 83%, which mainly were octadecatrienoic acid (about 61.7%), octadecadienoic acid (about 13.5%), octadecenoic acid (about 4.2%) and hexadecenoic acid (about 2.3%). In addition, 12 kinds of fatty acid, such as tetradecanoate (C14:0), hexadecanoic acid(C16:0), hexadecenoic acid (C16:1), Octadecanoic acid (C18:0), octadecenoic acid (C18:1), octadecadienoic acid(C18:2), octadecatrienoic acid(C18:3), were are always presnet, accounting for 98.2% to 99.2%, and were the main components of the plasma membrane. As the temperature decreases, the typy and content of fatty acids were changed too. 2-Butenedioic acid (E) and tetradecynoic acid appeared in summer. As autumn came, 12 kinds of fatty acid occurred, for example benzoic acid, dodecanoic acid and decanoic acid, their content was minimal. Moreover, diene fatty acid content decreased and the triene fatty acids increased. The results showed that the plasma membrane of Poa pratensis L. leaf contains higher unsaturated fatty acid, which has stronger tolerance to chill. In order to resist the cold temperature in autumn, the increase of unsaturation fatty acid enhances its resistance to chill.

Key words: Poa pratensis L., 2-Butenedioic acid (E), Palmitic acid, Linoleic acid, Linolenic acid, Heptadecanoic acid

摘要：

为了探索冷季型草坪草的耐冷机制，以气相色谱-质谱（GC-MS）对秋季草地早熟禾叶片脂肪酸进行测定，并与盛夏时作对比。利用NIST97.L质谱库共检索出27种脂肪酸，以棕榈酸和硬脂酸为主的饱和脂肪酸有13种，约占总数的17%；以亚麻酸、亚油酸和棕榈油酸为主的不饱和脂肪酸有14种，约占83%。此外，肉豆蔻酸、棕榈酸、油酸等12种脂肪酸始终存在，占98.2%～99.2%，是质膜的主要组分。随气温降低，脂肪酸种类和含量都发生了改变。盛夏有反丁烯二酸和十四炔酸，而入秋后出现了苯甲酸、月桂酸、癸酸等12种脂肪酸，但含量甚微；此外，二烯脂肪酸下降，三烯脂肪酸增加。研究结果表明，草地早熟禾叶片质膜含有较高的不饱和脂肪酸，具有较强的耐冷性；为了抵御秋季冷凉气温，其脂肪酸不饱和度进一步增加更是提高了对冷害的抗性。

关键词: 草地早熟禾, 反丁烯二酸, 棕榈酸, 亚油酸, 亚麻酸, 十七烷酸

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