Acta Agrestia Sinica

Acta Agrestia Sinica ›› 2019, Vol. 27 ›› Issue (5): 1231-1236.DOI: 10.11733/j.issn.1007-0435.2019.05.015

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The Relieving Effects on Germination Blocked of Medicago sativa Seed under Soda Saline-Alkali Stress of Soak Seed in ABA

LI Bo1, WU Ting-ting1, LI Hong2, YANG Zhao2, LIN Hao1   

  1. 1. College of Agriculture, Forestry and Life Sciences, Qiqihar University, Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Qiqihar, Heilongjiang Province 161006, China;
    2. Institute of Animal Husbandry and Veterinary of Heilongjiang academy of Agricultural sciences, Qiqihar, Heilongjiang Province 161005, China
  • Received:2019-08-13 Revised:2019-09-01 Online:2019-10-15 Published:2019-11-09

ABA浸种对混合苏打盐碱胁迫下苜蓿种子萌发受阻的缓解作用

李波1, 邬婷婷1, 李红2, 杨曌2, 林浩1   

  1. 1. 齐齐哈尔大学生命科学与农林学院, 抗性基因工程与寒地生物多样性保护黑龙江省重点实验室, 黑龙江 齐齐哈尔 161006;
    2. 黑龙江省农业科学院畜牧兽医分院, 黑龙江 齐齐哈尔 161005
  • 作者简介:李波(1962-),女,辽宁鞍山人,教授,硕士生导师,主要从事细胞生物学研究,E-mail:libo1962@163.com
  • 基金资助:
    黑龙江省应用技术研究与开发计划重大项目（GA18B104，GA19B103）；黑龙江省省属高等学校基本科研业务费科研项目（135209267，YSTSXK201886）；齐齐哈尔市科技计划项目（NYGG-201916）；2019年黑龙江省大学生创新创业训练计划资助项目（201910232095）；现代农业产业技术体系建设专项资金（CARS-34）资助

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Abstract: In this study,the seeds of WL343HQ and Longmu 807 alfalfa (Medicago sativa L.) were treated with 20,25,30,35,40,45,50,55 and 60 mmol·L-1 soda saline-alkali and 25,50,75 and 100 μmol·L-1 Abscisic acid.The effects of different doses of soda saline-alkali on germination rate of alfalfa seed and ABA relieving of soda saline-alkali stress on germination index of alfalfa seed were determined.The results showed that the germination rate of two alfalfa decreased with the increase of soda saline-alkali concentration. ABA relieved the stress of WL343HQ and Longmu 807 induced by soda saline-alkali at 40 and 35 mmol·L-1. WL343HQ was more resistance to soda saline-alkali when compared with Longmu 807;With the increase of ABA concentration,the germination rate,germination potential,germination index,vigor index,embrgo length,radicle length,fresh weight and dry weight of two alfalfa seeds increased first and then decreased. ABA,especially at the concentration of 75 μmol·L-1,relieved the stress of germination of alfalfa seeds induced by soda saline-alkali. This study provides a reference for alfalfa planting in soda salinized soil.

Key words: Alfalfa, Soda saline-alkali stress, Seed germination, Abscisic acid, Relieving effects, Membership function

摘要： 本研究以WL343HQ和龙牧807苜蓿（Medicago sativa L.）品种种子为试验材料，对其种子进行20，25，30，35，40，45，50，55和60 mmol·L-1苏打盐碱胁迫和25，50，75和100 μmol·L-1脱落酸（Abscisic acid，ABA）缓解处理，测定了不同苏打盐碱浓度对苜蓿种子发芽率和ABA缓解苏打盐碱胁迫对苜蓿种子萌发指标的影响。结果表明：2种苜蓿种子发芽率均随苏打盐碱胁迫浓度增加呈下降趋势，ABA缓解WL343HQ和龙牧807苏打盐碱胁迫浓度分别为40 mmol·L-1和35 mmol·L-1，WL343HQ抗苏打盐碱能力高于龙牧807；随ABA浓度的增加，2种苜蓿种子的发芽率、发芽势、发芽指数、活力指数和芽苗的胚芽长、胚根长、鲜重、干重均呈先升后降趋势。不同ABA浓度对苏打盐碱胁迫下的紫花苜蓿种子萌发均产生一定的缓解作用，特别是ABA浓度为75 μmol·L-1时缓解作用最明显。本研究结果可为苏打盐渍化土壤苜蓿种植提供一定参考。

关键词: 苜蓿, 苏打盐碱胁迫, 种子萌发, 脱落酸(ABA), 缓解作用, 隶属函数

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