Effects of Exogenous ABA on Photosynthetic Characteristics of Lolium perenne under Drought Stress

doi:10.11733/j.issn.1007-0435.2019.05.017

Acta Agrestia Sinica ›› 2019, Vol. 27 ›› Issue (5): 1243-1249.DOI: 10.11733/j.issn.1007-0435.2019.05.017

Previous Articles Next Articles

Effects of Exogenous ABA on Photosynthetic Characteristics of Lolium perenne under Drought Stress

XU Zhe¹, REN Jian², TIAN Ying¹, MI Nan¹

1. College of Agriculture, Ningxia University, Yinchuan, Ningxia 750021, China;
2. Department of Grass Science, Yunnan Agricultural University, Kunming, Yunnan Province 650201, China

Received:2019-07-29 Revised:2019-08-25 Online:2019-10-15 Published:2019-11-09

外源ABA对干旱胁迫下多年生黑麦草光合特性的影响

许喆¹, 任健², 田英¹, 米楠¹

1. 宁夏大学农学院, 宁夏银川 750021;
2. 云南农业大学草业科学系, 云南昆明 650201

通讯作者: 米楠
作者简介:许喆(1992-),女,山东泰安人,博士研究生,主要从事草地生态学研究,E-mall:496409847@qq.com
基金资助:
宁夏自治区重点研发项目（2018BEB04007）资助

Abstract

Abstract: In order to explore the effects of exogenous Abscisic acid (ABA) on the drought resistance of Lolium perenne under drought stress. A pot experiment was conducted to simulate different degrees of drought stress on the Lolium perenne variety "Dong Mu" using Polyethylene glycol (PEG). The effects of exogenous ABA at 0,200 and 500 μmol·L^-1 on photosynthetic characteristics and chlorophyll fluorescence parameters of Lolium perenne under normal,moderate and severe drought stress were studied by LI-6400XT Photosynthetic-fluorescence analyzer. The results showed that:Drought significantly affected the chlorophyll content and photosynthetic characteristics of Lolium perenne (P<0.05). Exogenous ABA at 500 μmol·L^-1 significantly increased the chlorophyll content of plant under severe drought. ABA only affected stomatal conductance (Gs) (P<0.05). ABA relieved the damage caused by drought stress on leaves. Exogenous ABA reduced the effects of drought stress on Fo,Fm,Fv,Fv/Fm,Fv/Fo and NPQ of Lolium perenne (P<0.05) and the damaged PSII reaction center can be quickly repaired,thus reduced the damage of chloroplast photosynthetic apparatus under drought stress.

Key words: Lolium perenne, Drought stress, Abscisic acid, Photosynthesis, Chlorophyll fluorescence

摘要： 为探究干旱胁迫下外源脱落酸（Abscisic acid，ABA）对多年生黑麦草（Lolium perenne）抗旱性的影响。本研究以多年生黑麦草品种“冬牧”为材料，采用盆栽试验，利用聚乙二醇（Polyethylene glycol，PEG）模拟不同程度的干旱胁迫，通过LI-6400XT光合-荧光测定仪，研究了正常、中度和重度干旱胁迫下外源0，200和500 μmol·L^-1的ABA对多年生黑麦草光合特性及叶绿素荧光参数的影响。结果表明，干旱对多年生黑麦草的叶绿素含量和光合特性产生了显著影响（P<0.05），重度干旱下共喷施225 ml的ABA 500 μmol·L^-1能够显著提高叶绿素含量，而ABA仅对气孔导度（Gs）产生了影响（P<0.05）。外源ABA能缓解干旱胁迫对多年生黑麦草初始荧光（Fo）、最大荧光（Fm）、可变荧光（Fv）、PSII最大光化学量子产量（Fv/Fm）、PSII潜在活性（Fv/Fo）和光化学猝灭（NPQ）的影响（P<0.05），使受损的PSII反应中心快速得到修复，从而降低干旱胁迫对叶绿体光合机构的破坏。

关键词: 多年生黑麦草, 干旱胁迫, 脱落酸, 光合作用, 叶绿素荧光

CLC Number:

S543+.6

XU Zhe, REN Jian, TIAN Ying, MI Nan. Effects of Exogenous ABA on Photosynthetic Characteristics of Lolium perenne under Drought Stress[J]. Acta Agrestia Sinica, 2019, 27(5): 1243-1249.

许喆, 任健, 田英, 米楠. 外源ABA对干旱胁迫下多年生黑麦草光合特性的影响[J]. 草地学报, 2019, 27(5): 1243-1249.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2019.05.017

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2019/V27/I5/1243

References

[1] 张爱玲,付晨,陈志宏,等. 8份柳枝稷种质资源苗期抗旱性综合评价[J]. 草业科学,2017,34(04):706-713
[2] 刘丹,姜中珠,陈祥伟. 水分胁迫下脱落酸的产生、作用机制及应用研究进展[J]. 东北林业大学学报,2003,(01):34-38
[3] 刘德兵,魏军亚,崔百明,等. 脱落酸对香蕉幼苗抗寒性的影响[J]. 热带作物学报,2007,28(2):1-4
[4] 张华,刘子会,马春红,等. 干旱胁迫下玉米木质部汁液pH和ABA含量变化及其与气孔关系[J]. 河北农业科学,2004,(02):35-39
[5] 鲁旭东,吴顺. 脱落酸对植物生长发育的调控作用[J]. 孝感学院学报,2004,(03):10-14
[6] 于锡宏,蒋欣梅,刁艳,等. 脱落酸、水杨酸和氯化钙对番茄幼苗抗冷性的影响[J]. 东北农业大学学报,2010,41(5):42-46
[7] 郭金生,曹丽茹,张新,等. 外源ABA对干旱胁迫下玉米叶片光合能力及气孔开度的影响[J]. 中国农学通报,2019,35(13):31-35
[8] Zhou Li,Yu Jingjin,Penga Yan,et al. Metabolic pathways regulated by abscisic acid,salicylic acid and raminobutyric acid in association with improved drought tolerance in creeping bentgrass (Agrostis stolonifera). Phsiologia Plantarum,2017,159:42-58
[9] 项洪涛,齐德强,李琬,等. 低温胁迫下外源ABA对开花期水稻叶鞘激素含量及抗寒生理的影响[J]. 草业学报,2019,28(04):81-94
[10] 严寒,许本波,赵福永,等. 脱落酸和水杨酸对干旱胁迫下芝麻幼苗生理特性的影响[J]. 干旱地区农业研究,2008(06):163-166+172
[11] 汤日圣,唐现洪,钟雨,等. 生物源脱落酸(ABA)提高茄苗抗旱能力的效果及机理[J]. 江苏农业学报,2006(01):10-13
[12] 李长宁,Manoj Kumar SRIVASTAVA,农倩,等. 水分胁迫下外源ABA提高甘蔗抗旱性的作用机制[J]. 作物学报,2010,36(05):863-870
[13] 安玉艳,梁宗锁. 植物应对干旱胁迫的阶段性策略[J]. 应用生态学报,2012,(10):2907-2915
[14] 马博英. 多年生黑麦草的逆境生理研究进展[J]. 生物学杂志,2010,(02):58-61
[15] 赵忠祥. 多年生黑麦草的栽培技术[J]. 河北农业科技,2007,(09):7
[16] 邱永福,田志宏. 湖北江汉平原冷季型草坪草的抗性研究[J]. 草业科学,2005,(09):77-80
[17] 张磊,刘东燕,邵涛. 黑麦草的饲用价值及其应用前景[J]. 草业科学,2008,(04):64-69
[18] 成文竞,崔建宇,闵凡华,等. 三种草坪草的根系分布特征及其对土壤养分的影响[J].草业学报,2009,18(01):179-183.
[19] 尚明娟,曹允馨,王刚,等. 干旱胁迫下多年生黑麦草对污泥处理的生理生化响应[J]. 草原与草坪,2018,38(02):25-32
[20] 梁小红,艾非凡,钟天秀,等. 多年生黑麦草对干旱-低温交叉适应的生理响应[J]. 草业学报,2016,25(01):163-170
[21] 梁国玲,周青平,颜红波. 聚乙二醇对羊茅属4种植物种子萌发特性的影响研究[J]. 草业科学,2007(06):50-54
[22] Inskeep W P,Bloom P R. Extinction Coefficients of Chlorophyll a and b in N,N-Dimethylformamide and 80% Acetone[J]. Plant Physiology,1985,77(2):483-485
[23] 焦旭亮,张振文,惠竹梅. "Li-6400光合作用测定仪"在葡萄上应用的商榷[J]. 西北农业学报,2007(01):209-212
[24] 刘志梅,蒋文伟,杨广远,等. 干旱胁迫对3种金银花叶绿素荧光参数的影响[J]. 浙江农林大学学报,2012,29(04):533-539
[25] 金微微,王炎,张会慧,等. 干旱胁迫下不同施氮水平对烤烟幼苗光合系统PSII功能的影响[J]. 南京农业大学学报,2012,35(04):21-26
[26] Farooq M,Wahid A,Kobayashi N,et al. Plant drought stress:Effects,mechanisms and management[J]. Sustainable Agriculture,2009,29(1):185-212
[27] 闫天芳,苗彦军,王向涛,等. 干旱胁迫对西藏4份野生披碱草属牧草幼苗生理指标的影响[J]. 草地学报,2017,25(06):1333-1339
[28] 种培芳,曾继娟,单立山,等. 干旱胁迫下荒漠草地植物红砂幼苗对外源ABA的生理响应[J]. 草地学报,2016,24(05):1001-1008
[29] 刘三梅,杨清辉,陈朝在,等. 干旱胁迫对甘蔗不同生育时期光合特性的影响[J]. 云南农业大学学报(自然科学),2016,31(03):398-403
[30] 张艳侠. 外源甜菜碱改善花生抗旱性的研究[D]. 泰安:山东农业大学,2006;21-22
[31] 殷云刚. 油菜素内酯和甜菜碱诱导番茄幼苗抗旱机理的研究[D]. 重庆:西南大学,2008;16-21
[32] 孙哲,范维娟,刘桂玲,等. 干旱胁迫下外源ABA对甘薯苗期叶片光合特性及相关生理指标的影响[J]. 植物生理学报,2017,53(05):873-880
[33] Zhang J Z,Shi L,Shi A P,et al. Photosynthetic Responses of Four Hosta,Cultivars to Shade Treatments[J]. Photosynthetica,2004,42(2):213-218
[34] B. J. Legg,W. Day,D. W. Lawlor,et al. The effects of drought on barley growth:models and measurements showing the relative importance of leaf area and photosynthetic rate[J]. Journal of Agricultural Science,1979,92(3):703-716
[35] 刘美君,隋晓青,安沙舟. 干旱脱水对狗牙根叶片光合机构的影响[J]. 草地学报,2018,26(02):441-44.
[36] 孙梅霞,祖朝龙,徐经年. 干旱对植物影响的研究进展[J]. 安徽农业科学,2004,32(2):365-367
[37] 顾振瑜,胡景江,文建雷,等. 元宝枫对干旱适应性的研究[J]. 西北林学院学报,1999,14(2):1-6
[38] 王书宏,杜永吉. 外源激素对干旱胁迫下草莓光合特性的影响[J]. 中国农学通报,2008,24(12):367-371
[39] 陈露露. 干旱胁迫下外源钙与脱落酸对黄瓜幼苗生长及生理特性的影响[D]. 泰安:山东农业大学,2016;27-30
[40] 郭贵华,刘海艳,李刚华,等. ABA缓解水稻孕穗期干旱胁迫生理特性的分析[J]. 中国农业科学,2014,47(22):4380-4391
[41] 许长成,程炳嵩,邹琦. 植物水分胁迫与活性氧代谢[J]. 山东农业大学学报,1993(01):113-117
[42] 李雪梅,陈强,王兰兰,等. 脱落酸对小麦幼苗光合及抗氧化酶的影响[J]. 沈阳师范大学学报(自然科学版),2006(02):221-223
[43] 刘小金,徐大平,杨曾奖,等. 脱落酸对檀香幼苗生长、光合及叶片抗氧化酶活性的影响[J]. 南京林业大学学报(自然科学版),2016,40(03):57-62
[44] 姚春娟,郭圣茂,马英超,等. 干旱胁迫对4种决明属植物光合作用和叶绿素荧光特性的影响[J]. 草业科学,2017,34(9):1880-1888
[45] 裴斌,张光灿,张淑勇,等. 土壤干旱胁迫对沙棘叶片光合作用和抗氧化酶活性的影响[J]. 生态学报,2013,33(5):1386-1396
[46] 付士磊,周永斌,何兴元,等. 干旱胁迫对杨树光合生理指标的影响[J]. 应用生态学报,2006,17(11):2016-2019
[47] 綦伟,谭浩,翟衡. 干旱胁迫对不同葡萄砧木光合特性和荧光参数的影响[J]. 应用生态学报,2006,17(5):835-838
[48] 王飞,刘世增,康才周,等. 干旱胁迫对沙地云杉光合、叶绿素荧光特性的影响[J]. 干旱区资源与环境,2017,(01):142-147
[49] 代微然,任健,墨继光. 干旱胁迫对假俭草叶绿素荧光特性的影响[J]. 草原与草坪,2010,30(05):1-5
[50] 许大全,张玉忠,张荣铣. 植物光合作用的光抑制[J]. 植物生理学报,1992(4):237-243
[51] 张军,王建波,陈刚,等. Na₂CO₃胁迫下星星草幼苗叶片电解质外渗率与PSⅡ光能耗散的关系[J]. 草业学报,2009,18(03):200-206
[52] 黄引娣. 六个桑橙种源抗旱抗盐机理研究[D]. 南京林业大学,2007
[53] 孔兰静. 三种观赏草对土壤干旱胁迫的生理响应[D]. 山东农业大学,2009
[54] 胡化广,张振铭,季芳芳,等. 干旱胁迫对结缕草叶绿素荧光和光合关键酶活性的影响[J].草地学报,2016,24(06):1304-1308
[55] 吴甘霖,段仁燕,王志高,等. 干旱和复水对草莓叶片叶绿素荧光特性的影响[J]. 生态学报,2010,30(14):3941-3946
[56] 谌端玉,欧静,王丽娟,等. 干旱胁迫对接种ERM真菌桃叶杜鹃幼苗叶绿素含量及荧光参数的影响[J]. 南方农业学报,2016,47(07):1164-1170
[57] 史胜青,袁玉欣,张金香,等. 不同水分胁迫方式对核桃苗叶绿素荧光动力学特性的影响[J].河北农业大学学报,2003(02):20-24
[58] 王玉萍,高会会,刘悦善,等. 高山植物光合机构耐受胁迫的适应机制[J]. 应用生态学报,2013,(07):2049-2055

Effects of Exogenous ABA on Photosynthetic Characteristics of Lolium perenne under Drought Stress

外源ABA对干旱胁迫下多年生黑麦草光合特性的影响

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZUO Lang, WANG Shu-sen, MA Ying-mei, WEN Su-ya-le-tu, BAI Gang, YU Xin-ming. Effect of Torch Tree Extract on Seeds Germination in Two Types of Turfgrass [J]. Acta Agrestia Sinica, 2021, 29(9): 1927-1933.
[2]	TONG Li-rong, NI Shun-gang, REN Xing-yuan, LIU Jia-xin, WEN Jing, WANG Juan, SONG Yu. Effects of Melatonin on Seedling Growth and Leaf Water Physiology of Lespedeza davurica under Drought Stress [J]. Acta Agrestia Sinica, 2021, 29(8): 1682-1688.
[3]	WANG Bao-qiang, ZHAO Ying, ZHU Xiao-lin, WANG Wang-tian, WEI Xiao-hong. Effects of Saline-alkali Stress on Photosynthesis Characteristics and Antioxidant System in Quinoa Seedlings Leaf [J]. Acta Agrestia Sinica, 2021, 29(8): 1689-1696.
[4]	MA Yin-shan, CHANG Yan, WANG Han-rui, JIA Yun-feng, LI Wen-ying, QI Rui-qiang. The Allelopathy of Hyoscyamus niger Seed Extract on Seed Germination and Seedling Growth of Lolium perenne [J]. Acta Agrestia Sinica, 2021, 29(8): 1697-1703.
[5]	LI Peng-zhen, ZHAO De-qin, DENG Bo, Yang Jun, Zhao Guo-hua, Zhao Liang. Effects of Biochar Addition and Drought Stress on Photosynthetic Efficiency and Growth of Alfalfa Inoculated with Rhizobia [J]. Acta Agrestia Sinica, 2021, 29(6): 1257-1264.
[6]	SUN Hua-fang, LI Xi-lai, LI Cheng-yi, ZHANG Jing, LIN Chun-ying, JIN Li-qun, YANG Xin-guang. Changes of CO₂ Fluxes and Chlorophyll Fluorescence Parameters of Biological Soil Crusts in a 19-year Artificial Grassland in the Source Zone of the Yellow River [J]. Acta Agrestia Sinica, 2021, 29(5): 894-903.
[7]	QIN Jin-ping, LIU Ying, MA Yu-shou, WANG Yan-long, LI Shi-xiong. Effects of Rest Grazing on Vegetation Characteristics and Photosynthetic Physiology of Dominant Species in Degraded Alpine Grasslands During Greenup Period [J]. Acta Agrestia Sinica, 2021, 29(5): 1034-1042.
[8]	LIANG Jia, MAYINU·Wusiman, FANG Zhi-gang. Effects of Exogenous Growth Substances on Seed Germination of Sweet Sorghum under Drought Stress [J]. Acta Agrestia Sinica, 2021, 29(3): 610-617.
[9]	YE Xiao-qian, LIU Rui-xi, FENG Jin-hui, DENG Shi-ming, LI Ji-tao, DENG Zhi-jun. Effect of Mucilage of Plantago asiatica Seeds on Its Deterioration and Germination Resistance [J]. Acta Agrestia Sinica, 2021, 29(12): 2728-2732.
[10]	YU Shu-yan, ZHOU Yan-fei, HUANG Wei, WANG Xing, GAO Xue-qin, FU Bing-zhe. Effects of Drought,Salt,and Drought-salt Combined Stress on Photosynthetic and Physiological Characteristics of Agropyron mongolicum [J]. Acta Agrestia Sinica, 2021, 29(11): 2399-2406.
[11]	QI Chen-xi, YI Guan-tao, FU Xiao-xuan, WANG Hai-long, WANG Jia-lin, WU Jian-hui. Effects of Arbuscular Mycorrhizal Fungi on Physiological Indexes of Potentilla anserina under Drought-Stress [J]. Acta Agrestia Sinica, 2021, 29(11): 2407-2412.
[12]	WANG Jian-qiu, CAO Zi-lin, WANG Xiao-li, ZHANG Jin-long, PENG Chen-yin. Effects of Lead Stress on Growth,Photosynthetic Physiology and Chlorophyll Fluorescence of Silene viscidula [J]. Acta Agrestia Sinica, 2021, 29(11): 2422-2427.
[13]	QIN Li-gang, LI Xue, LI Wei-yao, HU Yao, LI Jun, CUI Guo-wen. Effects of PEG Stress on Osmotic Regulation Substances and Enzyme Activities during Seed Germination of Three Allium L. [J]. Acta Agrestia Sinica, 2021, 29(1): 72-79.
[14]	LI Ying, YU Xiao-jun, ZHAO Yi-shan, WANG Lin, PENG Zhen, FAN Li-hua, WANG Yu-xia, MA Xiao-dong. Effects of Seed Soaking with Salicylic Acid and Abscisic Acid on Seed Germination and Seedling Growth of Medicago ruthenica at Low Temperature [J]. Acta Agrestia Sinica, 2021, 29(1): 174-181.
[15]	ZHANG Ran, MA Xiang, ZHU Rui-ting, NIU Kui-ju, ZHAO Chun-xu, MA Hui-ling. Metabolic Pathway and Transcriptional Regulation of Qinghai Wild Poa pratensis in Response to Drought Stress [J]. Acta Agrestia Sinica, 2020, 28(6): 1508-1518.