Expression Regulation of Monocopper Oxidase-like Protein SKU5 Gene and its Role in Alfalfa Fall Dormancy

doi:10.11733/j.issn.1007-0435.2019.06.005

Acta Agrestia Sinica ›› 2019, Vol. 27 ›› Issue (6): 1494-1502.DOI: 10.11733/j.issn.1007-0435.2019.06.005

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Expression Regulation of Monocopper Oxidase-like Protein SKU5 Gene and its Role in Alfalfa Fall Dormancy

DU Hong-qi¹, XU Zhao-xue¹, FANG Wei-ping³, FENG Chang-song¹, LOU Zhi-guo¹, YUAN Hua-yi¹

1. Institute of Animal Husbandry and Veterinary Research, Henan Academy of Agricultural Sciences, Zhengzhou, Henan Province 450008, China;
2. Henan Key Laboratory of Farm Animal Breeding and Nutritional Regulation, Zhengzhou, Henan Province 450008, China;
3. Economic Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou, Henan Province 450008, China

Received:2019-06-04 Revised:2019-09-07 Online:2019-12-15 Published:2019-12-31

苜蓿SKU5基因的表达调控及其在秋眠中的作用

杜红旗¹, 徐照学¹, 房卫平³, 冯长松¹, 娄治国¹, 袁华祎¹

1. 河南省农业科学院畜牧兽医研究所, 河南郑州, 450008;
2. 河南省畜禽繁育与营养调控重点实验室, 河南郑州, 450008;
3. 河南省农业科学院经济作物研究所, 河南郑州, 450008

通讯作者: 冯长松,E-mail:fengchangsong72@163.com
作者简介:杜红旗(1981-),男,汉,河南睢县人,助理研究员,博士,主要从事牧草分子生理和转基因研究,E-mail:biodhq@126.com
基金资助:
河南省博士后科研项目启动经费（19030086）；国家肉牛牦牛产业技术体系（CARS-37）资助

Abstract

Abstract: To explore the expression of SKU5 gene in alfalfa (Medicago sativa L.) leaves and its role in alfalfa fall dormancy,we detected the mRNA relative abundance of SKU5 in leaves of fall dormant alfalfa and non-dormant alfalfa in spring,summer and autumn,and at different temperature or different daylength under artificial conditions. Differences of SKU5 mRNA relative abundance in leaves of two fall dormancy type alfalfa varieties at the same growth stage and the seasonal changes of SKU5 gene expression were analyzed. The correlations among SKU5 mRNA relative abundance,leaf area,plant height,daylength and temperature was analyzed. The results showed that SKU5 gene expression was negatively correlated with daylength and positively correlated with temperature. The response of SKU5 gene expression to daylength and temperature were different between the two alfalfa varieties. SKU5 mRNA relative abundance in leaves of fall dormant alfalfa variety was negatively correlated with plant height and leaf area. It was speculated that the high-expression of SKU5 promoted the fall dormancy of alfalfa with the shortening of daylength.

Key words: Monocopper oxidase-like protein SKU5, Alfalfa, Fall dormancy, Gene expression, Correlation

摘要： 为了探索SKU5基因在紫花苜蓿（Medicago sativa L.）中表达调控规律和在苜蓿秋眠中的作用，本研究拟检测春、夏、秋季和人工条件下不同温度、不同日照长度下秋眠型和非秋眠型苜蓿叶片中SKU5基因mRNA相对表达量，分析同一秋眠型苜蓿品种不同时期SKU5基因mRNA相对表达量差异和不同时期2种秋眠型苜蓿品种间SKU5基因mRNA相对表达量的差异，以及SKU5基因mRNA相对表达量与苜蓿株高、叶面积、日照长度、温度的相关性。结果表明：SKU5基因的表达受日照长度极显著负调控，受温度极显著正调控；2种苜蓿叶片中SKU5基因的表达受日照长度和温度的调控结果不一致；秋眠型苜蓿叶中SKU5基因mRNA相对表达量与株高、叶面积呈显著负相关。因此，随着日照长度的缩短，SKU5基因高表达很可能促进秋眠型苜蓿的秋眠。

关键词: 单铜氧化酶样蛋白SKU5, 苜蓿, 秋眠性, 基因表达, 相关性

CLC Number:

S963.22+3.3

DU Hong-qi, XU Zhao-xue, FANG Wei-ping, FENG Chang-song, LOU Zhi-guo, YUAN Hua-yi. Expression Regulation of Monocopper Oxidase-like Protein SKU5 Gene and its Role in Alfalfa Fall Dormancy[J]. Acta Agrestia Sinica, 2019, 27(6): 1494-1502.

杜红旗, 徐照学, 房卫平, 冯长松, 娄治国, 袁华祎. 苜蓿SKU5基因的表达调控及其在秋眠中的作用[J]. 草地学报, 2019, 27(6): 1494-1502.

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Expression Regulation of Monocopper Oxidase-like Protein SKU5 Gene and its Role in Alfalfa Fall Dormancy

苜蓿SKU5基因的表达调控及其在秋眠中的作用

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