Effects of Nitrogen and Phosphorus Addition on Chlorophyll Content and Photosynthetic Activity of Nitraria tangutorum

doi:10.11733/j.issn.1007-0435.2020.03.013

Acta Agrestia Sinica ›› 2020, Vol. 28 ›› Issue (3): 694-702.DOI: 10.11733/j.issn.1007-0435.2020.03.013

Previous Articles Next Articles

Effects of Nitrogen and Phosphorus Addition on Chlorophyll Content and Photosynthetic Activity of Nitraria tangutorum

LIU Cheng-gong^1,2, SUN Gao-jie^1,2, DUAN Na³, LI Qing-he^1,2, ZHU Tian-tian^1,2, LI Hui-qing^1,2

1. Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China;
2. Key Laboratory of Tree Breeding and Cultivation, National Forestry and Grassland Administration, Beijing 100091, China;
3. Experiment Center of Desert Forestry, Chinese Academy of Forestry, Dengkou, Inner Mongolia 015200, China

Received:2019-12-22 Revised:2020-02-19 Online:2020-06-15 Published:2020-05-30

氮磷添加对唐古特白刺叶绿素含量和光合活性的影响

刘成功^1,2, 孙高洁^1,2, 段娜³, 李清河^1,2, 朱甜甜^1,2, 李慧卿^1,2

1. 中国林业科学研究院林业研究所, 北京 100091;
2. 国家林业和草原局林木培育重点实验室, 北京 100091;
3. 中国林业科学研究院沙漠林业实验中心, 内蒙古磴口 015200

通讯作者: 李清河
作者简介:刘成功(1990-),男,安徽合肥人,博士研究生,主要从事植物逆境生理生态学研究,E-mail:liucgwlq@163.com;
基金资助:
国家重点研发计划专题（2016YFC05009083）；国家自然科学基金（31470622）；中央级公益性科研院所基本科研业务费专项资金（CAFYBB2017MA021）共同资助

Abstract

Abstract: In this study,we used the method of chlorophyll fluorescence imaging and chlorophyll fluorescence dynamics to explore the effects of exogenous nitrogen and phosphorus addition on chlorophyll content,chlorophyll fluorescence parameters and fluorescence ratio R_fd images of the typical desert plant,Nitraria tangutorum. The results showed that the chlorophyll content in the leaves of N. tangutorum decreased significantly (P<0.05) when 5,10 and 15 g N·m^-2 nitrogen combined with 3 gP·m^-2phosphorus were added. With the increase of phosphorus addition,the chlorophyll content in the leaves of N. tangutorum trended to increase first and then decrease,the maximum chlorophyll content reached when 6 g P·m^-2 phosphorus were provided. There was no difference among F_m,F_v,F_v/F_m and R_fd gradients (P>0.05),and exogenous nitrogen and phosphorus did not damage the reaction center of PSⅡ. In addition,The CFI technology showed when 3 g P·m^-2 was added,the fluorescence ratio R_fd of N. tangutorum was the largest and the fluorescence intensity was the strongest,and the fluorescence was stronger in the middle and upper part of the leaves. Under fixed nitrogen level,the P3 had better photochemical efficiency,and the fluorescence intensity from above petiole to middle part was stronger. Therefore,it was concluded that exogenous nitrogen and phosphorus addition have coupling effect on chlorophyll content and fluorescence activity of N. tangutorum,and 3 g N·m^-2 nitrogen with 6 g P·m^-2 phosphorus can improve the chlorophyll content and fluorescence activity. By means of CFI technology,we can directly see the distribution characteristics of chlorophyll fluorescence on the leaves of N. tangutica. It provides a method for finding the stress symptoms of desert plants in time.

Key words: Nitraria tangutorum, Nitrogen and phosphorus additions, Photosynthetic activity, Chlorophyll fluorescence imaging

摘要： 本文采用叶绿素荧光成像和叶绿素荧光动力学相结合的方法研究外源氮（nitrogen，N）、磷（phosphorus，P）对荒漠植物唐古特白刺（Nitraria tangutorum）叶绿素含量、叶绿素荧光参数、荧光比R_fd的影响。结果表明：在添加P为3 gP·m^-2下，添加N为5 gN·m^-2，10 gN·m^-2和15 gN·m^-2时白刺的叶绿素含量显著下降（P < 0.05）；随着P的添加，叶绿素含量呈先增加后减小趋势，P3时（3 gN·m^-2+6 gP·m^-2）最大；外源N、P对白刺的F_m，F_v，F_v/F_m和R_fd的影响不显著，说明外源N、P未损害PSⅡ反应中心；单施P时，白刺的R_fd最大，荧光强度最强，而P3时的光化学效率较好，叶柄以上至中部的荧光较强。因此，本研究得出外源N、P对白刺叶绿素含量和荧光活性的影响具有耦合效应，N添加为3 gN·m^-2下配施6 gP·m^-2能够改善白刺的叶绿素含量和荧光活性；叶绿素荧光成像技术可直观地看出白刺的叶绿素荧光分布特征，为及时发现荒漠植物的胁迫症状提供了方法。

关键词: 唐古特白刺, 氮磷添加, 光合活性, 叶绿素荧光成像

CLC Number:

S793.9

LIU Cheng-gong, SUN Gao-jie, DUAN Na, LI Qing-he, ZHU Tian-tian, LI Hui-qing. Effects of Nitrogen and Phosphorus Addition on Chlorophyll Content and Photosynthetic Activity of Nitraria tangutorum[J]. Acta Agrestia Sinica, 2020, 28(3): 694-702.

刘成功, 孙高洁, 段娜, 李清河, 朱甜甜, 李慧卿. 氮磷添加对唐古特白刺叶绿素含量和光合活性的影响[J]. 草地学报, 2020, 28(3): 694-702.

0
/ / Recommend

Add to citation manager EndNote|Ris|BibTeX

URL: https://manu40.magtech.com.cn/Jweb_cdxb/EN/10.11733/j.issn.1007-0435.2020.03.013

https://manu40.magtech.com.cn/Jweb_cdxb/EN/Y2020/V28/I3/694

References

[1] 黄彩霞,柴守玺,赵德明,等. 氮磷肥配施对冬小麦灌浆期光合参数及产量的影响[J]. 植物学报,2015,50(1):47-54
[2] Cakmak I,Engels C,Rengel Z. Role of mineral nutrients in photosynthesis and yield formation[J]. Mineral Nutrition of Crops Fundamental Mechanisms & Implications,1999:141-168
[3] Perchlik M,Tegeder M. Leaf amino acid supply affects photosynthetic and plant nitrogen use efficiency under nitrogen stress[J]. Plant Physiology,2018,178(1):174-188
[4] Yang C,Yang L,Ouyang Z,et al. Studies on coordination of soil phosphorus supply with photosynthesis of rice under different nutrient treatments[J]. Journal of Plant Nutrition,2005,27(10):1717-1734
[5] 邢丹,李淑文,夏博,等. 磷肥施用对冬小麦产量及土壤氮素利用的影响[J]. 应用生态学报,2016,26(2):437-442
[6] Zhang L,Hoshika Y,Carrari E,et al. Effects of nitrogen and phosphorus imbalance on photosynthetic traits of poplar oxford clone under ozone pollution[J]. Journal of Plant Research,2018,131(6):915-924
[7] Okebalama C B,Safo E Y,Yeboah E,et al. Vegetative and reproductive performance of maize to nitrogen and phosphorus fertilizers in Plinthic Acrisol and Gleyic Plinthic Acrisol[J]. Journal of Plant Nutrition,2019,42(6):559-579
[8] 李彬,洪滔,陈欣凡,等. 氮梯度加载下闽楠幼苗生长与叶绿素荧光变化[J]. 森林与环境学报,2019,39(3):241-247
[9] 李绍长,胡昌浩,龚江,等. 低磷胁迫对磷不同利用效率玉米叶绿素荧光参数的影响[J]. 作物学报,2004,30(4):365-370
[10] Hidaka A,Kitayama K. Divergent patterns of photosynthetic phosphorus-use efficiency versus nitrogen-use efficiency of tree leaves along nutrient-availability gradients[J]. Journal of Ecology,2009,97(5):984-991
[11] Elumeeva T G,Onipchenko V G,Wu Y. Leaf functional traits of plants of alpine pastures at the Eastern Qinghai-Tibetan Plateau[J]. Moscow University Biological Sciences Bulletin,2015,70(1):46-52
[12] Zhao M,Yu K. Application of chlorophyll fluorescence technique in the study of coral symbiotic zooxanthellae micro-ecology[J]. Acta Ecologica Sinica,2014,34(3):165-169
[13] Lichtenthaler H K,Babani F. Detection of photosynthetic activity and water stressby imaging the red chlorophyll fluorescence[J]. Plant Physiology and Biochemistry (Paris),2000,38(11):889-895
[14] 罗光宏,王进,颜霞,等. 干旱胁迫对唐古特白刺(Nitraria tangutorum)种子吸胀萌发和幼苗生长的影响[J]. 中国沙漠,2014(6):1537-1543
[15] 段娜,李清河,陈晓娜,等. 模拟干旱和氮沉降对唐古特白刺根系生长特征的影响[J]. 草地学报,2019,27(4):956-961
[16] 李海涛,曹芳,张东梅. 唐古特白刺叶化学成分的研究[J]. 中成药,2018,40(7):86-89
[17] Wang N,Gao J,Zhang S Q,et al. Variations in leaf and root stoichiometry of Nitraria tangutorum,along aridity gradients in the Hexi Corridor,northwest China[J]. Contemporary Problems of Ecology,2014,7(3):308-314
[18] 陈志强,李庆贱,时瑞亭,等. 苏打盐碱胁迫对西伯利亚白刺光合和生长的影响[J]. 北京林业大学学报,2011(3):31-37
[19] 王林龙,李清河,徐军,等. 沙埋对白刺表型可塑性的影响[J]. 林业科学研究,2016,29(3):442-447
[20] 鲍芳,何季,曹燕丽,等. 荒漠植物白刺光合作用对人工模拟增雨的响应与适应[J]. 中国科学院大学学报,2017,34(4):508-514
[21] Li Q,Xu J,Li H,et al. Effects of aspect on clonal reproduction and biomass allocation of layering modules of Nitraria tangutorum in Nebkha Dunes[J]. Plos One,2013,8(10):e79927
[22] 张俊菲. 白刺芽库动态对氮添加的响应研究[D]. 北京:中国林业科学研究院,2017:34-35
[23] 薛海霞. 白刺生物量分配和养分含量对施肥的响应[D]. 北京:中国林业科学研究院,2016:33-34
[24] 张进霞,刘晓静,郝凤,等. 氮磷调控对紫花苜蓿氮积累与土壤氮磷营养的影响[J]. 草地学报,2016,24(1):61-68
[25] Lichtenthaler H K,Babani F,Langsdorf G,et al. Measurement of differences in red chlorophyll fluorescence and photosynthetic activity between sun and shade leaves by fluorescence imaging[J]. Photosynthetica,2000,38(4):521-529
[26] Jakob T,Schreiber U,Kirchesch V,et al. Estimation of chlorophyll content and daily primary production of the major algal groups by means of multiwave length-excitation PAM chlorophyll fluorometry:Performance and methodological limits[J]. Photosynthesis Research,2005,83(3):343-361
[27] 徐培洲,李云,袁澍,等. 叶绿素缺乏水稻突变体中光系统蛋白和叶绿素合成特性的研究[J]. 中国农业科学,2006,39(7):1299-1305
[28] 陈继辉,李炎朋,熊雪,等. 氮磷添加对草地土壤酸度和化学计量学特征的影响[J]. 草业科学,2017,34(5):943-949
[29] Lyu X T,Reed S,Yu Q,et al. Convergent responses of nitrogen and phosphorus resorption to nitrogen inputs in a semiarid grassland[J]. Global Change Biology,2013,19(9):2775-2784
[30] 詹书侠,陈伏生,胡小飞,等. 中亚热带丘陵红壤区森林演替典型阶段土壤氮磷有效性[J]. 生态学报,2009,29(9):4673-4680
[31] Stevens C J,Dise N B,Mountford J O,et al. Impact of nitrogen deposition on the species richness of grasslands[J]. Science,2004,303(5665):1876-1879
[32] Zhan S X,Wang Y,Zhu Z C,et al. Nitrogen enrichment alters plant N:P stoichiometry and intensifies phosphorus limitation in a steppe ecosystem[J]. Environmental & Experimental Botany,2016(134):21-32
[33] 张芸,李惠通,张辉,等. 不同林龄杉木人工林土壤C:N:P化学计量特征及其与土壤理化性质的关系[J]. 生态学报,2019,39(7):2520-2531
[34] 齐敏兴,刘晓静,张晓磊,等. 不同磷水平对紫花苜蓿光合作用和根瘤固氮特性的影响[J]. 草地学报,2013,21(3):512-516
[35] Wu B J,Chow W S,Liu Y J,et al. Effects of stomatal development on stomatal conductance and on stomatal limitation of photosynthesis in Syringa oblata and Euonymus japonicus Thunb.[J]. Plant Science,2014(229):23-31
[36] 张志录,刘中华,彭舜磊,等. 氮磷添加对考来木光合特性和叶绿素荧光的影响[J]. 浙江农林大学学报2019,36(3):459-467
[37] Liao Y,Xu D Q. Novel evidence for a reversible dissociation of light-harvesting complex II from photosystem II reaction center complex induced by saturating light illumination in soybean leaves[J]. Journal of Integrative Plant Biology,2007,49(4):523-530
[38] 杨文权,顾沐宇,寇建村,等. 干旱及复水对小冠花光合及叶绿素荧光参数的影响[J]. 草地学报,2013,21(6):1130-1135
[39] 周春艳,华灯鑫,乐静,等. 结合图像的叶绿素荧光动力学植物水分胁迫探测方法[J]. 农业机械学报,2017,48(1):153-159
[40] Gameiro C,Utkin A B,Cartaxana P,et al. The use of laser induced chlorophyll fluorescence (LIF) as a fast and non-destructive method to investigate water deficit in Arabidopsis[J]. Agricultural Water Management,2016(164):127-136
[41] 刘军,齐广平,康燕霞,等. 不同灌溉处理下紫花苜蓿光合特性、叶绿素荧光参数及生物量的变化[J]. 草地学报,2019,27(6):1569-1576
[42] 宋航,闫庆伟,巴雅尔图,等. 水氮交互对草地早熟禾叶绿素荧光和RuBisCO酶活力的影响[J]. 中国草地学报,2017,39(5):32-38
[43] 黄瑾,高志娟,王世琪,等. 氮素对分蘖期干旱及复水柳枝稷光合生理特性的影响[J]. 中国草地学报,2018,40(6):10-17
[44] 朱隆静,喻景权. 不同供磷水平对番茄生长和光合作用的影响[J]. 浙江农业学报,2005,17(3):120-122
[45] 林达定,张国防,于静波,等. 芳樟不同无性系叶片光合色素含量及叶绿素荧光参数分析[J]. 植物资源与环境学报,2011,20(3):56-61
[46] Lautner S,Grams T E E,Matyssek R,et al. Characteristics of electrical signals in poplar and responses in photosynthesis[J]. Plant Physiology,2005,138(4):2200-2209
[47] Berger S,Papadopoulos M,Schreiber U,et al. Complex regulation of gene expression,photosynthesis and sugar levels by pathogen infection in tomato[J]. Physiologia Plantarum,2004,122(4):419-428
[48] Gorbe E,Calatayud A. Applications of chlorophyll fluorescence imaging technique in horticultural research:a review[J]. Scientia Horticulturae,2012,138(2):24-35
[49] 邱宝平,康雪莉,郭世英,等. 不同品种晒烟叶片发育过程中碳氮代谢对施肥的响应[J]. 山东农业科学,2015(3):63-67
[50] 武维华. 植物生理学[M]. 北京:科学出版社,2003:90-111
[51] Lu C M,Zhang J H. Photosystem II photochemistry and its sensitivity to heat stress in maize plants as affected by nitrogen deficiency[J]. Journal of Plant Physiology,2000,157(1):124-130
[52] Mollier,A. Maize root system growth and development as influenced by phosphorus deficiency[J]. Journal of Experimental Botany,1999,50(333):487-497

Effects of Nitrogen and Phosphorus Addition on Chlorophyll Content and Photosynthetic Activity of Nitraria tangutorum

氮磷添加对唐古特白刺叶绿素含量和光合活性的影响

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 3

Recommended Articles

Metrics

Comments

[1]	YI Dan, WANG Bo, DUAN Hui-rong, LI Yi, WANG Li-rong. Identification and Expression Analysis of 14-3-3 Gene Family in Nitraria tangutorum [J]. Acta Agrestia Sinica, 2021, 29(3): 443-456.
[2]	DUAN Na, LI Qing-he, CHEN Xiao-na, GAO Jun-liang, DUO Pu-zeng, ZHANG Ran-hao, WANG Ji. Combined Effects of Simulated Drought Stress and Nitrogen Deposition on Root Growth Characteristics of Nitraria tangutorum [J]. Acta Agrestia Sinica, 2019, 27(4): 956-961.
[3]	HAN Yong-wei, TUO Xue-sen, GAO Xin-ting, GAO Ji-xi . A Comparative Study on Photosynthetic Characteristics of Haloxylon ammodendron and Nitraria tangutorum on Temperate steppa subdesert in Alashan [J]. , 2010, 18(3): 314-319.